한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
권호 표지

Characterization and Expression Pattern of the Partial Myostatin cDNA in Shrimp, Fenneropenaeus chinensis

  • Lee, Sang Beum (Division of Marine Molecular Biotechnology, Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Kim, Yong Soo (Department of Human Nutrition, Food and Animal Sciences, University of Hawaii) ;
  • Yoon, Moongeun (Division of Marine Molecular Biotechnology, Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Kim, Su-Kyoung (East Sea Fisheries Research Institute, National Fisheries Research & Development Institute) ;
  • Jang, In Kwon (West Sea Mariculture Research Center, National Fisheries Research & Development Institute) ;
  • Lim, Hyun Jeong (Research Plan Team, National Fisheries Research & Development Institute) ;
  • Jin, Hyung-Joo (Division of Marine Molecular Biotechnology, Faculty of Marine Bioscience and Technology, Kangnung National University)
  • 발행 : 2007.12.31
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초록

Muscle tissue expresses many muscle-specific genes, including myostatin (also known as GDF8) that is a member of the transforming growth factor-beta superfamily. Myostatin (MSTN) negatively regulates mammalian skeletal muscle growth and development by inhibiting myoblast proliferation. Mice and cattle possessing mutant MSTN alleles display a 'double muscling' phenotype characterized by extreme skeletal muscle hypertrophy and/or hyperplasia. In this study, we first have characterized partial cDNA of a MSTN gene from the muscle tissue in the F. chinensis and examined its expression pattern in various tissues. The partial MSTN gene (GenBank accession number EU 131093) in the F. chinensis was 1134 bp, encoding for 377 amino acids that showed 63-93% amino acid similarity to other vertebrate MSTNs, containing a conserved proteolytic cleavage site (RXRR) and conserved cysteine residues in the C-terminus. Based on a RT-PCR, the MSTN gene was expressed in the all tissues of F. chinensis used in this study.

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