Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Inhibition of Proliferation by Anti-microbial Peptide Isolated from Pediococcus pentosaceus and Lactobacillus spp. in Colon Cancer Cell Line (HT-29, SW 480 and Caco-2)

Pediococcus pentosaceus 및 Lactobacillus spp. 종의 유산균으로부터 분리한 항균 peptide들(Safelac and Lactopad)이 인간 결장암 세포주(HT-29, SW 480 and Caco-2)의 증식 억제에 미치는 효과

  • Published : 2007.03.31
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Abstract

유산균(Lactic acid bacteria)은 Escherichia coli와 Salmonella typhimurium과 같은 병원균에 대한 항균활성을 나타낼 뿐만 아니라 면역 증강효과를 나타내는 등 인체내에서 건강에 이로운 다양한 역할을 수행하는 것으로 알려졌다. 특히, Pediococcus pentosaseus와 몇몇 Lactobacillus 종으로부터 분리한 항균활성을 나타내는 peptide들인 safelac과 lactopad는 몇몇 암세포주의 성장을 억제하는 것으로 나타났다. 이에, 본 연구에서는 HT-29, SW 480 및 Caco-2와 같은 3종류의 인간의 결장암 세포주에 safelac과 lactopad를 투여하여 이들이 항암효과를 나타낼 수 있는 지를 분석하고자 하였다. XTT assay는 safelaf과 lactopad가 HT-29, SW 480 및 Caco-2의 성장을 억제하는 것으로 나타났으며, 특히, 이들 peptide들을 72시간동안 처리했을 때 나타나는 항암효과는 $3.1{\sim}100mg/mL$의 농도범위에서 유의한 결과를 나타내었으며, 분석한 농도 범위에서 용량 의존적인 방식으로 더 강한 효과를 나타내었다. RAW 264.7 세포주는 cytokine인 tumor-necrosis factor(TNF-${\alpha}$)의 생성에 미치는 이들 peptide들의 효과를 조사하기 위한 대식세포의 모델로써 이용되었다. RAW 264.7 세포주에서 TNF-${\alpha}$의 생성은 이들 peptide들에 의해 48시간 배양시 용량에 의존적인 방식으로 영향을 받는 것으로 나타났다. 따라서, 이러한 발견은 safelac과 lactopad와 같은 유산균으로부터 분리한 항균 peptide들이 결장암 세포에 대한 화학적 예방제로서의 잠재성을 갖고 있음을 시사하는 결과로서 주목된다.

Keywords

References

  1. Abdelali H, Cassand P, Soussotte V, Daubeze M, Bouley C and Narbonne JF. Effect of dairy products on initiation of precursor lesions of colon cancer in rats, Nutr Cancer 1995; 24: 121-132 https://doi.org/10.1080/01635589509514400
  2. Arimochi H, Kinouchi T, Kataoka K, Kusahara T and Ohnishi Y. Effect of intestinal bacteria on formation of azoxymethane-induced aberrant crypt foci in the rat colon, Biochem Biophys Res Commun 1997; 238: 753-757 https://doi.org/10.1006/bbrc.1997.7384
  3. de Simone C, Vesely R, Bianchi SB and Jirillo E. The role of probiotics in modulation of the immune system in man and in animals, Int J Immunotherapy 1993; 9: 23-28
  4. Fuller R. Probiotics in man and animal, J Appl Bacteriol 1989; 66: 365-378 https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  5. Gallaher DD, Stallings WH, Blessing LL, Busta FF and Brady LJ. Probiotics, cecal microflora, and aberrant crypts in the rat colon, J Nutr 1996; 126: 1362-1371
  6. Gibson GR, Rastall RA and Fuller R. The health benefits of probiotics and prebiotics. In: Fuller R, Perdigon G(Eds.), Gut Flora, Nutrition, Immunity and Health. Blackwell Publishing, Oxpord, 2003; 52-70
  7. Goldin BR and Gorbach SL. Effect of Lactobacillus acidophilus dietary supplements in 1, 2-dimethyhydrazine dihydrochloride-induced intestinal cancer in rats, J Natl Cancer Inst 1980; 64: 263-265 https://doi.org/10.1093/jnci/64.2.263
  8. Goldin BR, Gualtieri LJ and Moore RP. The effect of Lactobacillus GG on the initiation and promotion of DMHinduced intestinal tumors in the rat, Nutr Cancer 1996; 25: 197-204 https://doi.org/10.1080/01635589609514442
  9. Hirayama K and Rafter J. The role of probiotic bacteria in cancer prevention, Mirobes Infect 2000; 2: 681-686 https://doi.org/10.1016/S1286-4579(00)00357-9
  10. Kato I, Kobayashi S, Yokokura T and Mutai M. Antitumor activity of Lactobacillus casei in mic, Gann 1981; 72: 517-523
  11. Kato I, Yokokura T and Mutai M. Macrophage activation by Lactobacillus casei in mice, Microbiol Immunol 1983; 27: 611-618 https://doi.org/10.1111/j.1348-0421.1983.tb00622.x
  12. Kohwi T, Imai K, Tamura A and Hashimoto Y. Antitumor activity of Bifidobacterium infantis in mice, Gann 1978; 69: 613-618
  13. Maclennan R and Jensen OM. Dietary fibre, transit time, fecal bacteria, steroids, and colon cancer in two Scandinavian population, Lancet 1997; 30: 207-211
  14. Malhotra SL. Dietary factors in a study of colon cancer from Cancer Registry, with special reference to the role of saliva, milk and fermented milk products and vegetable fibre, Med Hypotheses 1997; 3: 122-126 https://doi.org/10.1016/0306-9877(77)90024-X
  15. Matsuzaki T. Immunomodulation by treatment with Lactobacillus casei strain Shirota, Int J Food Microbiol 1998; 41: 133-140 https://doi.org/10.1016/S0168-1605(98)00046-4
  16. Oda M, Hasegawa H, Komatsu S, Kambe M and Tsuchigy F. Anti-tumor polysaccharide from Lactobacillus sp., Agric Biol Chem 1983; 47: 1623-1625 https://doi.org/10.1080/00021369.1983.10865826
  17. Onazaki K, Matsuhima K, Kleinerman E, Sato T and Oppenheim J. The role of interleukin-1 in promoting human monocyte mediated tumor cytotoxicity, J Immunol 1985; 135: 314-320
  18. Onoue M, Kado S, Sakaitani Y, Uchida K and Morotomi M. Specific species of intestinal bacteria influence the induction of aberrant crypt foci by 1, 2-dimethylhydrazine in rats, Cancer Lett 1997; 113: 179-186 https://doi.org/10.1016/S0304-3835(97)04698-3
  19. Philip R and Epstein L. Tumor necrosis factor as immunomodulator and mediator of monocyte cytotoxicity induced by itself, ${\gamma}-interferon$ and interleukin-1, Nature 1986; 323: 86-89 https://doi.org/10.1038/323086a0
  20. Pisani P, Parkin DM and Ferlay J. Estimates of the worldwide mortality from eighteen major cancer in 1985, Int J Cancer 1993; 54: 594-606 https://doi.org/10.1002/ijc.2910540413
  21. Pool-Zobel BL, Neudecker C, Domizlaff I, Ji S, Schillinger U, Rumney C, Moretti M, Vilarini I, Scassellati-Sforzolini R and Rowland I. Lactobacillus- and Bifidobacterium-mediated antigenotoxicity in the colon of rats, Nutr Cancer 1996; 26: 365-380 https://doi.org/10.1080/01635589609514492
  22. Raitano A and Korc M. Growth inhibition of a human colorectal carcinoma cell line by interleukin-1 is associated with enhanced expression of ${\gamma}-interferon$ receptors, Cancer Res 1993; 53: 636-640
  23. Reddy GV, Friend BA, Shahani KM and Farmer RE. Antitumor activity of yogurt components, J Food Protect 1983; 46: 8-11 https://doi.org/10.4315/0362-028X-46.1.8
  24. Reddy GV, Shahani KM and Benerjee MR. Inhibitory effect of yogurt on Ehrlich ascites tumor cell proliferation, J Natl Cancer Inst 1973; 50: 815-817 https://doi.org/10.1093/jnci/50.3.815
  25. Rowland IR, Rumney CJ, Coutts JT and Lievense LC. Effect of Bifidobacterium longum and inulin on gut bacterial metabolism and carcinogen-induced aberrant crypt foci in rats, Carcinogenesis 1998; 19: 281-285 https://doi.org/10.1093/carcin/19.2.281
  26. Salminen S, Bouley C, Boutron-Ruault MC, Cummings JH, Frank A, Gibson GR, Isolauri E, Moreau MC, Roberfroid M and Rowland I. Functional food science and gastrointestinal physiology and function, Br J Nutr 1998; 80(supplement): s147-s171 https://doi.org/10.1079/BJN19980108
  27. Schiffrin EJ, Rochat F, Link-Amster H, Aeschlimann JM and Donnet-Hughes A. Immunomodulation of human blood cells following the ingestion of lactic acid bacteria, J Dairy Sci 1995; 78: 491-496 https://doi.org/10.3168/jds.S0022-0302(95)76659-0
  28. Sekine K, Ohta J, Onishi M, Tatsuki T, Shimokawa Y, Toida T, Kawashima T and Hashimoto Y. Analysis of antitumor properties of effector cells stimulated with a cell wall preparation (WPG) of Bifidobacterium infantis, Biol Pharm Bull 1995; 18: 148-153 https://doi.org/10.1248/bpb.18.148
  29. Shahani KM and Ayebo AD. Role of dietary lactobacilli in gastrointestinal microecology, Am J Clin Nutr 1980; 33: 2448-2457 https://doi.org/10.1093/ajcn/33.11.2448
  30. Singh J, Rivenson A, Tomita M, Shimamura S, Ishibashi N and Reddy BS. Bifidobacterium longum, a lactic acid producing intestinal bacterium inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis, Carcinogenesis 1997; 18: 833-841 https://doi.org/10.1093/carcin/18.4.833
  31. Strober W. Trypan blue exclusion test for cell viability. In : Coligan JE, Kruisbeek AM, Marguiles DH, Shevach EM, Strober W(Eds.), Current protocols in Immunology, Green Pub. And Wiley-Interscience, New York, 1991; A3.3-4
  32. You H, Oh DK and Ji GE. Anticancerogenic effect of a novel chiroinositol-containg polysaccharide from Bifidobacterium bifidum BGN4, FEMS Microbiol Lett 2004; 240: 131-136 https://doi.org/10.1016/j.femsle.2004.09.020
  33. Yu HJ, Lee SS, Lee DS and Kim HB. Isolation of Lactobacillus plantarum HB1 from Tongchimi and Its Nitrite Scavenging Effect, Kor J Microbiol 2003; 39: 192-196