Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Pulp Response of Mineral Trioxide Aggregate, Calcium Sulfate or Calcium Hydroxide

Mineral trioxide aggregate, calcium sulfate와 calcium hydroxide의 치수에 대한 반응

  • Yun, Young-ran (Department of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • Yang, In-Seok (Department of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • Hwang, Yun-Chan (Department of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • Hwang, In-Nam (Department of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • Choi, Hong-Ran (Department of Oral Pathology, School of Dentistry, Chonnam National University) ;
  • Yoon, Suk-Ja (Department of Oral and Maxillofacial Radiology, School of Dentistry, Chonnam National University) ;
  • Kim, Sun-Hun (Department of Oral Anatomy, DSRI, 2nd stage of BK21, School of Dentistry, Chonnam National University) ;
  • Oh, Won-Mann (Department of Conservative Dentistry, School of Dentistry, Chonnam National University)
  • 윤영란 (전남대학교치의학전문대학원 보존학교실) ;
  • 양인석 (전남대학교치의학전문대학원 보존학교실) ;
  • 황윤찬 (전남대학교치의학전문대학원 보존학교실) ;
  • 황인남 (전남대학교치의학전문대학원 보존학교실) ;
  • 최홍란 (전남대학교치의학전문대학원 구강악안면방사선학교실) ;
  • 윤숙자 (전남대학교치의학전문대학원 구강해부학교실) ;
  • 김선헌 (전남대학교치의학전문대학원 치의학연구소, 2단계 BK21 사업단) ;
  • 오원만 (전남대학교치의학전문대학원 보존학교실)
  • Published : 2007.03.31
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Abstract

This study was performed to verify the possibility of MTA and calcium sulfate as a pulp capping agent through comparing the dental pulp response in dogs after capping with MTA, calcium sulfate, and calcium hydroxide. 24 teeth of 2 dogs, 8 month old, were used in this study. Under general anesthesia, cervical cavities were prepared and pulp was exposed with sterilized #2 round bur in a high speed handpiece. MTA calcium hydroxide, and calcium sulfate were applied on the exposed pulp. Then the coronal openin,fs were sealed with IRM and light-cured composite. Two months after treatment, the animals were sacrificed. The extracted teeth were fixed in 10% neutral-buffered formalin solution and were decalcified in formic acid-sodium citrate. They were prepared for histological examination in the usual manner. The sections were stained with haematoxylin and eosin. In MTA group, a hard tissue bridges formation and newly formed odontoblasts layer was observed. There was no sign of pulp inflammatory reaction in pulp tissue. In calcium hydroxide group, there was no odontoblast layer below the dentin bridge. In pulpal tissue, chronic inflammatory reaction with variable intensity and extension occurred in all samples. In calcium sulfate group, newly formed odontoblast layer was observed below the bridge. Mild chronic inflammation with a few neutrophil infiltrations was observed on pulp tissue. These results suggest that MTA is more biocompatible on pulp tissue than calcium hydroxide or calcium sulfate.

개의 치수에 MTA, calcium hydroxide 및 calcium sulfate로 치수복조 후 치수 반응을 서로 비교하여 MTA와 calcium sulfate가 임상적으로 치수복조제로서 사용 가능할 것인가를 구명하고자 본 연구를 시행하였다. 8개월 된 2마리 개의 24개의 치아가 본 연구에 사용되었다 전신 마취하에 고속 핸드피스를 사용하여 멸균된 #2 round bur로 치경부에 와동을 형성한 후 치수를 노출시켰다. MTA, calcium hydroxide 및 calcium sulfate를 치수노출부에 도포하였다. 와동 부위는 IRM으로 가봉하고 광중합레진으로 수복하였다. 처리 2개월 후, 전신 마취하에 희생시킨 후 조직학적으로 관찰하였다. MTA처리군에서는 치수 노출부위에 경조직의 상아질교가 형성되었으며 새로 형성된 상아질교 하방에 조상아세포가 새로 형성되었다. 치수충혈과 함께 국소적 인 혈관 증식 이 나타났으며 치수에 염증반응은 나타나지 않았다. Calcium hydroxide로 처리한 군은 상아질교 하방에 조상아세포가 관찰되지 않았으며 만성 염증반응이 다양하게 나타났다. Calcium sulfate로 처리한 군은 경조직의 상아질교가 관찰되었으며, 상아질교 하방에 조상아세포 층이 새로 관찰되었다. 몇몇의 중성구 침윤과 함께 미약한 정도의 만성염증반응이 관찰되었다. 이상의 결과에서 MTA가 calcium hydroxide및 calcium sulfate에 비해 치수에 생체친화적임을 시사하며 기계적 치수노출시 치수복조제로 사용할 수 있음을 시사한다.

Keywords

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