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Antioxidant Activity of Theaflavin and Thearubigin Separated from Korean Microbially Fermented Tea

  • Shon, Mi-Yae (Department of Food and Nutrition, Gyeongsang National University) ;
  • Park, Seok-Kyu (Department of Food and Nutrition, Sunchon National University) ;
  • Nam, Sang-Hae (Department of Food Science, Jinju National University)
  • Published : 2007.03.31

Abstract

Theaflavins (TF) and thearubigins (TR) were separated from Korean microbially fermented tea leaves. Contents of TF (74.4 $\mu$M/g) and TR (37.2%) were higher than reported for black tea fermented by oxidase. Antioxidant activities of TF, TR and EGCG were analyzed and protective effects of COS-7 cells against copper and cadmium-induced toxicity were investigated. TF and TR exhibited good inhibition rates of about 85$\sim$90% for antioxidant and scavenging activities of free radicals and protected COS-7 cells against apoptosis or damage caused by stress, such as cadmium and copper-oxidative injury, free radicals etc. These results indicate that TF, TR and EGCG have antioxidant and scavenging activities against free radicals and protect COS-7 cells from Cu, Cd induced injury.

Keywords

References

  1. Shiraki M, Hara Y, Osawa T, Kumon H, Nakauma T, Kawaskishi S. 1994. Antioxidative and antimutagenic effects of theaflavins from black tea. Mutat Res 323: 29-34 https://doi.org/10.1016/0165-7992(94)90041-8
  2. Riemersma RA, Rice-Evans CA, Tyrrell RM, Clifford MN, Lean MEJ. 2001. Tea flavonoids and cardiovascular health. QJM 94: 277-282 https://doi.org/10.1093/qjmed/94.5.277
  3. Hultberg B, Anderson AI, Isaakson A. 1997. A novel example for the development of adaptive response against a toxicant. Toxicology 117: 89-97 https://doi.org/10.1016/S0300-483X(96)03554-8
  4. Koizumi T, Li ZG. 1992. Role of oxidative stress in single dose, cadmium-induced testicular cancer. J Toxicol Environ Health 37: 25-36 https://doi.org/10.1080/15287399209531654
  5. Muller L. 1986. Consequences of cadmium toxicity in rat hepatocytes: Mitochondrial dysfunction and lipid perox-idation. Toxicology 40: 285-292 https://doi.org/10.1016/0300-483X(86)90061-2
  6. Burkitt MJ. 2001. A critical over view of the chemistry of copper dependent low density lipoprotein oxidation: roles of lipid hydroperoxides, \alpha-tocopherol, thiols, and ceruloplasmin. Arch Biochem Biophys 394: 117-135 https://doi.org/10.1006/abbi.2001.2509
  7. Patterson RA, Lamb DJ, Leake DS. 2003. Mechanisms by which cysteine can inhibit or promote the oxidation of low density lipoprotein by copper. Atherosclerosis 169: 87-94 https://doi.org/10.1016/S0021-9150(03)00154-0
  8. Faris MW. 1991. Cadmium toxicity: unique cytoprotective properties of alpha tocopheryl succinate in hepatocytes. Toxicology 69: 63-77 https://doi.org/10.1016/0300-483X(91)90154-S
  9. Chow CK. 1991. Vitamin E and oxidative stress. Free Radic Biol Med 11: 215-232 https://doi.org/10.1016/0891-5849(91)90174-2
  10. Hilton PJ, Ellis RT. 1972. Estimation of market value of Central African tea by theaflavin analysis. J Agric Food Chem 50: 1833-1839 https://doi.org/10.1021/jf0114435
  11. Roberts EAH, Smith RF. 1961. Spectrophotometer measurements of theaflavins and thearubigins in black tea liquors in assessments of quality in teas. The Analyst 86: 94-98 https://doi.org/10.1039/an9618600094
  12. Shokri F, Heidari MSG, Ghazi-Khansari M. 2000. In vitro inhibitory effects of antioxidants on cytotoxicity of T-2 toxin. Toxicology 146: 171-176 https://doi.org/10.1016/S0300-483X(00)00172-4
  13. Halliwell B, Gutteridge JMC, Aruoma OI. 1987. The deoxyribose method: a simple 'test-tube' assay for determination of rate constants for reaction of hydroxyl radicals. Anaytical Biochemistry 165: 215-219 https://doi.org/10.1016/0003-2697(87)90222-3
  14. Miller HE. 1971. A simplified method for the evaluation of antioxidant. J Am Oil Chem Soc 18: 439-452 https://doi.org/10.1007/BF02635693
  15. Martin OP, Okinda O, Richard M, Mutuku MK. 2004. Changes in thearubigin fractions and theaflavin levels due to variations in processing conditions and their influence on black tea liquor brightness and total color. Food Chem 85: 163-173 https://doi.org/10.1016/S0308-8146(02)00183-8
  16. Shon MY, Kim SH, Park SK, Sung NJ. 2004. Antioxidant activity of solvent extracts from Korean fermented tea. Korean J Food Preser 11: 544-549
  17. Shon MY, Kim SH, Sung NJ. 2004. Antioxidant activity of Korean fermented tea extracts. J Life Science 14: 920-924 https://doi.org/10.5352/JLS.2004.14.6.920
  18. Michael D, Mattie J, Freedman H. 2001. Protective effects of aspirin and vitamin E against copper- and cadmium-induced toxicity. Biochem Biophys Res Commun 285: 921- 925 https://doi.org/10.1006/bbrc.2001.5259
  19. Romeo M, Bennani N, Gnassia-Barelli M, Lafaurie M, Girard JP. 2000. Cadmium and copper display different responses toward oxidative stress in the kidney of the sea bass Dicentrarchus labrax. Aquat Toxicol 48: 185-194 https://doi.org/10.1016/S0166-445X(99)00039-9
  20. Jayaprakasha GK, Singh RP, Sakariah KK. 2001. Antioxi-dant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem 73: 285-290 https://doi.org/10.1016/S0308-8146(00)00298-3

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