한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제36권3호
  • /
  • Pages.269-275
  • /
  • 2007
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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별불가사리 추출물의 면역세포 활성화 효과

Effect of Asterina pectinifera Extracts on the Activation of Immune Cells

  • 채수연 (순천대학교 생물학과) ;
  • 김미정 (순천대학교 생물학과) ;
  • 김도순 (순천대학교 생물학과) ;
  • 박정은 (순천대학교 생물학과) ;
  • 조성기 (한국원자력연구소 방사선연구원 방사선식품.생명공학팀) ;
  • 이성태 (순천대학교 생물학과)
  • Chae, Su-Yeon (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Kim, Mi-Jung (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Kim, Do-Soon (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Park, Jung-Eun (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Jo, Sung-Kee (Radiation Food and Biotechnology Team, Advanced Radiation Technology Institute, Jeongup Campus of Korea Atomic Energy Research Institute) ;
  • Yee, Sung-Tae (Dept. of Biology, College of Natural Science, Sunchon National University)
  • 발행 : 2007.03.31
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초록

불가사리 추출물을 이용하여 생쥐 비장에 있는 면역세포 활성화 효과에 대해 실험한 결과, 다음과 같은 결과를 얻었다. 첫째, 별불가사리 추출물 중에서 B세포와 대식세포를 활성화시키는 성분은 아세톤처리로 추출할 수 있었다. 둘째, 이 성분은 농도 의존적으로 생쥐 비장세포의 증식반응을 유도하였으며, IL-6와 $IFN-{\gamma}$의 생산을 유도하였다. 셋째, 이 성분은 B세포의 증식을 유도하였으며, 이 때 면역글로불린 IgM과 IgG의 생산도 유도하였다. 넷째, 이 성분은 대식세포주의 일산화질소 생산을 유도하였으며, 또 $TNF-{\alpha}$, GM- CSF와 IL-6의 분비를 유도하였다. 이상의 실험 결과, 본 실험에서 사용한 별불가사리 추출물에는 B세포와 대식세포 같은 면역세포의 증식과 각종 사이토카인을 생산을 유도하여, 면역반응을 조절하는 성분이 포함되어 있는 것으로 생각된다.

In this experiment, the effects of Asterina pectinifera extracts on the activation of immune cells were studied. An immune cell activating factor was partially purified from starfish, Asterina pectinifera, by means of physiological saline extraction, acetone precipitation and heating inactivation. Starfish extracts increased the proliferation of spleen cells and induced the production of IL-6 and $IFN-{\gamma}$ by spleen cells. Also, it increased the proliferation of purified B cells and production of IgM and IgG in the presence of Asterina pectinifera extracts. Starfish extract self-induced NO synthesis in mouse macrophage cell line (RAW264.7). When cell lines was treated with extracts, the mRNA expression of inducible NO synthetase (iNOS), $TNF-{\alpha}$, IL-6, and GM-CSF were markedly increased in RT-PCR analysis. Therefore starfish extract can self-activate spleen cells, B cells and macrophages. These results might be useful in further studies into a possible immune activating agent from the starfish, Asterina pectinifera, for the development of functional foods and drugs.

키워드

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