Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Statistical Optimization of Medium Components for the Production of Biosurfactant by Bacillus licheniformis K51

  • Joshi Joshi (Department of Microbiology and Biotechnology Centre, Maharaja Sayajirao University of Baroda) ;
  • Sanket Sanket (Department of Microbiology and Biotechnology Centre, Maharaja Sayajirao University of Baroda) ;
  • Yadav Sanjay (Department of Microbiology and Biotechnology Centre, Maharaja Sayajirao University of Baroda) ;
  • Nerurkar Anuradha (Department of Microbiology and Biotechnology Centre, Maharaja Sayajirao University of Baroda) ;
  • Desai Anjana J. (Department of Microbiology and Biotechnology Centre, Maharaja Sayajirao University of Baroda)
  • Published : 2007.02.28
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Abstract

The nutritional medium requirement for biosurfactant production by Bacillus licheniformis K51 was optimized. The important medium components, identified by the initial screening method of Plackett-Burman, were $H_3PO_4,\;CaCl_2,H_3BO_3$, and Na-EDTA. Box-Behnken response surface methodology was applied to further optimize biosurfactant production. The optimal concentrations for higher production of biosurfactants were (g/l): glucose, $1.1;NaNO_3,\;4.4;MgSO_4{\cdot}7H_2O,\;0.8;KCl,\;0.4;CaCl_2,\;0.27;H_3PO_4,\;1.0ml/l;\;and\;trace elements\;(mg/l):H_3BO_3,\;0.25;CuSO_4,\;0.6;MnSO_4,\;2.2;Na_{2}MoO_4,\;0.5;ZnSO_4,\;6.0;FeSO_4,\;8.0;CoCL_2,\;1.0;$ and Na-EDTA, 30.0. Using this statistical optimization method, the relative biosurfactant yield as critical micelle dilution (CMD) was increased from $10{\times}\;to\;105{\times}$, which is ten times higher than the non-optimized rich medium.

Keywords

References

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