Molecular & Cellular Toxicology

  • 제3권4호
  • /
  • Pages.320-325
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  • 2007
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  • 1738-642X(pISSN)
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  • 2092-8467(eISSN)
대한독성유전 단백체학회 (The Korean Society of Toxicogenomics and Toxicoproteomics)
권호 표지

The Relationship DNA Methylation of $p16^{INK4a}$ and Colorectal Cancer

  • Hong, Young-Seoub (Department of Preventive Medicine, College of Medicine, Dong-A University) ;
  • You, Chang-Hun (Department of Preventive Medicine, College of Medicine, Dong-A University) ;
  • Roh, Mee-Sook (Department of Pathology, College of Medicine, Dong-A University) ;
  • Kim, Na-Young (Department of Preventive Medicine, College of Medicine, Dong-A University) ;
  • Lee, Kyung-Eun (Department of Pathology, College of Medicine, Dong-A University) ;
  • Kim, Hyo-Jun (Department of Preventive Medicine, College of Medicine, Dong-A University) ;
  • Lee, Hyun-Jae (Department of Occupational Medicine, Dong-A University) ;
  • Kwak, Jong-Young (Medical Research Center for Cancer Molecular Therapy, College of Medicine, Dong-A University) ;
  • Kim, Joon-Youn (Department of Preventive Medicine, College of Medicine, Dong-A University)
  • 발행 : 2007.12.31
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초록

Promoter hypermethylation of the $p16^{INK4a}$ gene was investigated in 52 sets of samples of tumor tissue and adjacent normal tissue from Korean patients with colorectal cancer, using the proposed modified the Real-time PCR/SYBR Green detection method presented in this study. In normal tissue, 29 of 52 patients (56%) were methylated and in tumor tissue, 23 of 52 patients (44%) were methylated. The 34 cases (65.4%) showed a concordant DNA methylation pattern in both normal tissue and tumor tissue. Analyzing the association between the clinicopathologic features and DNA methylation status of the $p16^{INK4a}$ gene, the DNA methylation status according to by Duke's stage was different while other clinicopathological characteristics, including the age, sex, tumor stage, and histologic type of the patient were not found to be correlated with $p16^{INK4a}$ methylation. With multivariate logistic regression, it was observed that the DNA methylation status of $p16^{INK4a}$ gene in normal tissue was correlated with the DNA methylation status of the $p16^{INK4a}$ gene in tumor tissue (P=0.026). According to a Kaplan-Meier survival analysis, a difference in the survival rate by DNA methylation status was found, but it was not significant.

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참고문헌

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