Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells

  • Kim, Hyun-Ji (Department of Veterinary Medicine, College of Applied Life Sciences, Cheju National University) ;
  • Kim, Mi-Hyoung (Department of Veterinary Medicine, College of Applied Life Sciences, Cheju National University) ;
  • Byon, Yun-Young (Applied Radiological Science Research Institute, Cheju National University) ;
  • Park, Jae-Woo (Department of Nuclear and Energy Engineering, College of Engineering, Cheju National University) ;
  • Jee, Young-Heun (Department of Veterinary Medicine, College of Applied Life Sciences, Cheju National University) ;
  • Joo, Hong-Gu (Department of Veterinary Medicine, College of Applied Life Sciences, Cheju National University)
  • Published : 20070300
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Abstract

An acidic polysaccharide of Panax ginseng (APG), so called ginsan is known to have important immunomodulatory activities. It was recently reported that APG has radioprotective effects in mice but the detailed mechanism was not fully elucidated. This study examined the effects of APG on bone marrow cells (BMs). The phenotypical and functional changes in APG-treated BMs after gamma radiation were studied. The benefit of APG on BMs damaged by gamma radiation was determined by measuring the cell viability. Using 2 different assays, a pretreatment with APG significantly increased the viability of BMs against gamma radiation. APG-treated BMs had a significantly higher amount of IL-12, which is a major cytokine for immune responses, compared with the medium-treated BMs. The expression of MHC class II molecules of APG-treated BMs was also increased, and APG-treated BMs showed significantly higher levels of allogeneic CD4+ T lymphocyte proliferation. Furthermore, APG-treated mice had a larger number of BMs after gamma radiation than the control mice, and the BMs of APG-treated mice were successfully cultured into dendritic cells, which are the representative antigenpresenting cells. Overall, this study shows that APG alters the phenotype of BMs, increases the viability and alloreactivity of BMs after gamma radiation both in vitro and in vivo. Therefore, APG may be a good candidate radioprotective agent for BMs.

Keywords

Acknowledgement

This research was performed under the program of Basic Atomic Energy Research Institute (BAERI) which is a part of the Nuclear R&D Programs funded by the Ministry of Science & Technology (MOST) of Korea. APG was kindly provided by Dr. Jie-Young Song (Korea Institute of Radiological and Medical Sciences, Korea).

References

  1. Ahn JY, Choi IS, Shim JY, Yun EK, Yun YS, Jeong G, Song JY. The immunomodulator ginsan induces resistance to experimental sepsis by inhibiting Toll-like receptor-mediated inflammatory signals. Eur J Immunol 2006, 36, 37-45 https://doi.org/10.1002/eji.200535138
  2. Arora R, Gupta D, Chawla R, Sagar R, Sharma A, Kumar R, Prasad J, Singh S, Samanta N, Sharma RK. Radioprotection by plant products: present status and future prospects. Phytother Res 2005, 19, 1-22 https://doi.org/10.1002/ptr.1605
  3. Dalmau SR, Freitas CS, Savino W. Radio- and chemoprotection of bone marrow cells by opposite cell cycle-acting cytokines. Leuk Res 1997, 21, 93-99 https://doi.org/10.1016/S0145-2126(96)00052-5
  4. Han SK, Song JY, Yun YS, Yi SY. Ginsan improved Th1 immune response inhibited by gamma radiation. Arch Pharm Res 2005, 28, 343-350 https://doi.org/10.1007/BF02977803
  5. Han Y, Son SJ, Akhalaia M, Platonov A, Son HJ, Lee KH, Yun YS, Song JY. Modulation of radiation-induced disturbances of antioxidant defense systems by ginsan. Evid Based Complement Alternat Med 2005, 2, 529-536 https://doi.org/10.1093/ecam/neh123
  6. Ivanova T, Han Y, Son HJ, Yun YS, Song JY. Antimutagenic effect of polysaccharide ginsan extracted from Panax ginseng. Food Chem Toxicol 2006, 44, 517-521 https://doi.org/10.1016/j.fct.2005.08.032
  7. Joo HG, Goedegebuure PS, Sadanaga N, Nagoshi M, von Bernstorff W, Eberlein TJ. Expression and function of galectin-3, a beta-galactoside-binding protein in activated T lymphocytes. J Leukoc Biol 2001, 69, 555-564
  8. Joo SS, Won TJ, Kim MS, Lee DI. Hematopoietic effect of ginsenoside Rg3 in ICR mouse primary cultures and its application to a biological response modifier. Fitoterapia 2004, 75, 337-341 https://doi.org/10.1016/j.fitote.2004.02.008
  9. Kang KA, Zhang R, Lee KH, Chae S, Kim BJ, Kwak YS, Park JW, Lee NH, Hyun JW. Protective effect of triphlorethol-A from Ecklonia cava against ionizing radiation in vitro. J Radiat Res (Tokyo) 2006, 47, 61-68 https://doi.org/10.1269/jrr.47.61
  10. Kumar M, Sharma MK, Saxena PS, Kumar A. Radioprotective effect of Panax ginseng on the phosphatases and lipid peroxidation level in testes of Swiss albino mice. Biol Pharm Bull 2003, 26, 308-312 https://doi.org/10.1248/bpb.26.308
  11. Lee HJ, Kim SR, Kim JC, Kang CM, Lee YS, Jo SK, Kim TH, Jang JS, Nah SY, Kim SH. In Vivo radioprotective effect of Panax ginseng C.A. Meyer and identification of active ginsenosides. Phytother Res 2006, 20, 392-395 https://doi.org/10.1002/ptr.1867
  12. Lee TK, Johnke RM, Allison RR, O'Brien KF, Dobbs LJ Jr. Radioprotective potential of ginseng. Mutagenesis 2005, 20, 237-243 https://doi.org/10.1093/mutage/gei041
  13. Lee YS, Chung IS, Lee IR, Kim KH, Hong WS, Yun YS. Activation of multiple effector pathways of immune system by the antineoplastic immunostimulator acidic polysaccharide ginsan isolated from Panax ginseng. Anticancer Res 1997, 17, 323-331
  14. Mori M, Desaintes C. Gene expression in response to ionizing radiation: an overview of molecular features in hematopoietic cells. J Biol Regul Homeost Agents 2004, 18, 363-371
  15. Neta R. Modulation with cytokines of radiation injury: suggested mechanisms of action. Environ Health Perspect 1997, 105 (Suppl 6), 1463-1465 https://doi.org/10.2307/3433652
  16. Rades D, Fehlauer F, Bajrovic A, Mahlmann B, Richter E, Alberti W. Serious adverse effects of amifostine during radiotherapy in head and neck cancer patients. Radiother Oncol 2004, 70, 261-264 https://doi.org/10.1016/j.radonc.2003.10.005
  17. Santini V, Giles FJ. The potential of amifostine: from cytoprotectant to therapeutic agent. Haematologica 1999, 84, 1035-1042
  18. Shin JY, Song JY, Yun YS, Yang HO, Rhee DK, Pyo S. Immunostimulating effects of acidic polysaccharides extract of Panax ginseng on macrophage function. Immunopharmacol Immunotoxicol 2002, 24, 469-482 https://doi.org/10.1081/IPH-120014730
  19. Song JY, Akhalaia M, Platonov A, Kim HD, Jung IS, Han YS, Yun YS. Effects of polysaccharide ginsan from Panax ginseng on liver function. Arch Pharm Res 2004, 27, 531- 538 https://doi.org/10.1007/BF02980127
  20. Song JY, Han SK, Bae KG, Lim DS, Son SJ, Jung IS, Yi SY, Yun YS. Radioprotective effects of ginsan, an immunomodulator. Radiat Res 2003, 159, 768-774 https://doi.org/10.1667/0033-7587(2003)159[0768:REOGAI]2.0.CO;2
  21. Wrembel-Wargocka J, Jablonska H, Chomiczewski K. Clinical use of amifostine (WR-2721) as a preparation protecting healthy tissues from the cytotoxic effects of chemotherapy and radiation therapy. Przegl Lek 1996, 53, 820-825
  22. Zhang JS, Sigdestad CP, Gemmell MA, Grdina DJ. Modification of radiation response in mice by fractionated extracts of Panax ginseng. Radiat Res 1987, 112, 156-163 https://doi.org/10.2307/3577086