Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Increased α2,3-Sialylation and Hyperglycosylation of N-Glycans in Embryonic Rat Cortical Neurons During Camptothecin-induced Apoptosis

  • Kim, Sung-Min (Department of Biotechnology and Biomaterial Engineering Center, The Catholic University of Korea) ;
  • Lee, Jung-Sun (Department of Biotechnology and Biomaterial Engineering Center, The Catholic University of Korea) ;
  • Lee, Yoon-Hee (Department of Biotechnology and Biomaterial Engineering Center, The Catholic University of Korea) ;
  • Kim, Woo-Jung (Department of Biotechnology and Biomaterial Engineering Center, The Catholic University of Korea) ;
  • Do, Su-Il (Department of Biological Science, Ajou University) ;
  • Choo, Young-Kug (Department of Biological Science, Wonkwang University) ;
  • Park, Yong-Il (Department of Biotechnology and Biomaterial Engineering Center, The Catholic University of Korea)
  • Received : 2007.08.01
  • Accepted : 2007.09.13
  • Published : 2007.12.31
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Abstract

Alterations in the glycan chains of cell surface glycoconjugates are frequently involved biological processes such as cell-cell interaction, cell migration, differentiation and development. Cultured embryonic (E18) rat cortical neurons underwent apoptosis in response to camptothecin, and lectin histochemistry showed that binding to apoptotic neurons of FITC-conjugated Maackia amurensis agglutinin (MAA), which is specific for terminal ${\alpha}2,3$-sialic acid residues, increased progressively with increasing concentrations of camptothecin. Analysis of the total proteins of apoptotic neurons by SDS-PAGE, and lectin blotting using HRP-labeled MAA, revealed that the expression of terminal ${\alpha}2,3$-sialic acid residues on an unknown protein with an apparent molecular mass of 25.6 kDa also increased in apoptotic neurons. NP-HPLC analysis of the total cellular N-glycans of normal and apoptotic neurons demonstrated that the expression of structurally simpler biantennary types of N-glycans fell by 49% during apoptosis whereas the more branched triantennary types of N-glycans with terminal sialic acid residues increased by up to 59%. These results suggest that increased surface expression of ${\alpha}2,3$-sialic acid residues and hyperglycosylation of N-glycans is a common feature of cellular responses to changes in cell physiology such as tumorigenesis and apoptosis.

Keywords

Acknowledgement

Supported by : Korea Science and Engineering Foundation (KOSEF)

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