한국어병학회지 (Journal of fish pathology)

  • 제20권1호
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  • Pages.71-81
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  • 2007
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

In vitro에서 Photobacterium damselae subsp. damselae와 Vibrio 속 세균의 병원성 비교

Comparative study of Photobacterium damselae subsp. damselae and Vibrios on pathogenicity in vitro

  • 권문경 (국립수산과학원 병리연구팀) ;
  • 김명석 (국립수산과학원 병리연구팀) ;
  • 조병열 (울산지방해양수산청) ;
  • 김진우 (국립수산과학원 병리연구팀) ;
  • 박수일 (부경대학교 수산생명의학과)
  • Kwon, Mun-Gyeong (Pathology Team, National Fisheries Research and Development Institute) ;
  • Kim, Myoung-Sug (Pathology Team, National Fisheries Research and Development Institute) ;
  • Cho, Byoung-Youl (Ulsan Regional Maritime Affairs and Fisheries office) ;
  • Kim, Jin-Woo (Pathology Team, National Fisheries Research and Development Institute) ;
  • Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
  • 발행 : 2007.04.30
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초록

넙치, Paralichthys olivaceus 병어에서 분리된 Photobacterium damselae subsp. damselae와 Vibrio spp.(V. anguillarum, V. splendidus, V. harveyi and V. ordalii)의 병원성을 in vitro에서 조사하였다. Extracellular products (ECPs)의 성상은 효소 활성, 양과 넙치 적혈구에 대한 용혈능과 cell-line에 대한 세포 독성을 조사하였다. 그리고 세균의 internalization에서 signal transduction pathways를 signal transduction inhibitor를 이용하여 조사하였다.P. damselae는 phospholipase 활성, 넙치 적혈구에 대한 용혈능, 세포독성이 높게 나타났으며, 분리된 vibrios 균주에 비하여 internalization에서 다양한 경로를 나타내었다. 따라서 이러한 활성은 P. damselae의 병원성과 관련이 있을 것으로 생각된다.

Photobacterium damselae subsp. damselae and 4 Vibrio spp.(V. anguillarum, V. splendidus, V. harveyi and V. ordalii) were isolated from the diseased olive flounders, Paralichthys olivaceus. The isolates were tested on the pathogenicity in vitro. The properties of extracellular products(ECPs) were investigated with enzymatic activities, hemolytic activities toward the sheep and olive flounder erythrocytes, and cytotoxicity activities on the cell-line. And potential signal transduction pathways of the bacterial internalization were detected by using signal transduction inhibitors. P. damselae was high in phospholipase activity, hemolytic activity to olive flounder erythrocytes and cytotoxicity activity. And P. damselae had diversified internalizing pathways as compared to isolated vibrios. Therefore, these activities may be related with pathogenicity of P. damselae.

키워드

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