Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Developmental Proteomic Profiling of Porcine Skeletal Muscle during Postnatal Development

  • Kim, Nam-Kuk (Department of Applied Biochemistry, College of Biomedical & Health Science Konkuk University) ;
  • Lim, Jong-Hyun (Department of Applied Biochemistry, College of Biomedical & Health Science Konkuk University) ;
  • Song, Min-Jin (Department of Applied Biochemistry, College of Biomedical & Health Science Konkuk University) ;
  • Kim, Oun-Hyun (Department of Animal Science, College of Natural Science, Konkuk University) ;
  • Park, Beom-Young (National Institute of Animal Science, Rural Development Admistration) ;
  • Kim, Myung-Jick (National Institute of Animal Science, Rural Development Admistration) ;
  • Hwang, In-Ho (Department of Animal Resources and Biotechnology, Chonbuk National University) ;
  • Lee, Chang-Soo (Department of Applied Biochemistry, College of Biomedical & Health Science Konkuk University)
  • Received : 2006.12.15
  • Accepted : 2007.03.19
  • Published : 2007.10.01
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Abstract

In this study, we have compared the skeletal muscle proteome at various stages of porcine postnatal development. Korean native pigs were divided into five postnatal stages of 30, 70, 130, 170 and 300 d and their loin muscles were analyzed for muscle proteome by using two-dimensional electrophoresis and mass spectrometry. We found 5 proteins showing a consistent pattern during skeletal muscle growth. Four proteins were identified as myosin light chain 1 slow-twitch (MLC1sa) isoform, troponin T, triosephosphate isomerase (TIP) and DJ-1 protein. The remaining protein was not identified. Two muscle fiber proteins of MLC1sa isoform and troponin T showed a high expression level at an early postnatal stage and then their levels were decreased markedly during growth stages. On the other hand, the expression of TIP and DJ-1 protein, which are well known as catalysis enzyme and antioxidant-related protein, respectively, were linearly increased during growth stages. Thus, the stage-related muscle proteins may be useful as parameters for understanding the developmental characteristics of biochemical and physiological properties in Korean native pig skeletal muscle.

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References

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