DOI QR코드

DOI QR Code

Study of sweat content analysis and latent fingerprint developing

땀의 성분 분석과 잠재지문 현출에 관한 연구

  • Choi, Mi-Jung (Dept. of Scientific Criminal Investigation, Chungnam National University) ;
  • Sun, Yale-Shik (Hazard Evaluation Center Korea Testing & Research Institute) ;
  • Kim, Chang-Seong (Hazard Evaluation Center Korea Testing & Research Institute) ;
  • Choi, Man-Sik (Dept. of Oceanography and Marine Environmental Science, Chungnam National University) ;
  • Sung, Nack-Do (Dept. of Applied Biology, Chemistry & Food Science, Chungnam National University) ;
  • Park, Sung-Woo (Dept. of Scientific Criminal Investigation, Chungnam National University)
  • Received : 2007.02.16
  • Accepted : 2007.03.22
  • Published : 2007.04.28

Abstract

Sweat contents were investigated for using those data to forensic purpose. The experiments of identifying sweat contents were as follow: 1) measurement of amino acids (aspartic acid, serine, glycine etc) by HPLC, 2) anions ($Cl^-$, $F^-$, and $SO{_4}^{2-}$) by IC and 3) trace elements (Cu, Zn, Li, B, etc.) by ICP-MS. Amino acid contents in sweat are varied with individual samples and glycine, threonine, alanine, valine and histidine are detected as the prime one. The detected anions are $Cl^-$(2167~4073 ppm) and $F^-$(454~582 ppm) mostly. Trace elements of Rb, Zn and Cu are detected and those concentrations are relatively very high. The compositions of sweat can be influenced by various factors (diet, anthropometric, characteristics, physical fitness, age, gender and the state of the health).

잠재지문의 효과적인 현출법의 기초자료로 활용하기 위하여 지문과 관계되는 땀의 성분분석을 실시하였다. 땀의 성분을 분석한 결과 총 단백질은 $46{\sim}122{\mu}L/mL$로 개인차가 있고, 검출된 아미노산의 분포도 개인에 따라 그 차이가 있으며, 주로 glycine($4.7{\sim}19.0{\mu}L/mL$), threonine($1.3{\sim}26.0{\mu}L/mL$), alanine($0{\sim}13.5{\mu}L/mL$), valine($0{\sim}8.0{\mu}L/mL$) 그리고 histidine($0{\sim}13.2{\mu}L/mL$) 등이 검출되었다. 양이온의 경우 Na이 3078.6~4815.6 ppm으로 가장 높은 함량을 보였고, K이 267.3~823.9 ppm이었으며, Ca(15.4~44.7 ppm)과 Mg(4.13~8.96 ppm)으로 조사되었다. 음이온 중에는 $Cl^-$(2167~4073 ppm)와 $F^-$(454~582 ppm)가 주로 검출되었다. 미량 원소는 Rb(132~824 ppb), Zn(90~1846 ppb)와 Cu(17~415 ppb)가 주로 검출 되었으며, 그 양 역시 개인 간의 차이가 크게 나타나므로 지문 현출에 다양한 방법이 요구되어 진다.

Keywords

References

  1. T. Hirokawa, H. Okamoto, Y. Gosyo, T. Tsuda and A. R. Timerbaev, Analytica chimica Acta, 581, 83-88 (2007) https://doi.org/10.1016/j.aca.2006.07.077
  2. S. Naruse, H. I Shiguro, T. Tsuda, Pancreas, 28, 80-85 (2004) https://doi.org/10.1097/00006676-200401000-00013
  3. D. Thieme, P. Anielski, J. Grosse, H. Sachs and R. K. Mueller, Analytica chimica Acta, 483, 299-306 (2003) https://doi.org/10.1016/S0003-2670(02)01604-5
  4. V. V. Lierde, C. C. Chery, L. Moens and F. Vanhaecke, Electrophoresis, 26, 1703-1711 (2005) https://doi.org/10.1002/elps.200410221
  5. C. palacios, K. Wigertz, B. Martin, C. M. Weaver, Nutrition Research, 23, 401-411 (2003) https://doi.org/10.1016/S0271-5317(02)00546-8
  6. P. Mehnert, P. Brode and B. Griefahn, International J. Industrial Ergonomics, 29, 343-351 (2002) https://doi.org/10.1016/S0169-8141(02)00073-2
  7. T. Verde, R. P. Corey and R. Moore, J. Appl. physiol, 53, 1540-1545 (1982) https://doi.org/10.1152/jappl.1982.53.6.1540
  8. Lee, Ju-Rip, The korean J of physical Education, 42, 809-815 (2003)
  9. J. Malchaire, B. Kampmann, G .Havenith, P. Mehnertend. H. Gebhardt, International Archives of Occuptional and Environmental Health, 73, 215-220 (2000). https://doi.org/10.1007/s004200050420
  10. C. G. Worley, S. S. Wilthshire, T. C. Miller, GJ. Havrilla and V. Majidi, J. of Forensic Sci., 51, 57-63 (2007)
  11. A. I. Ektessabi, Y. Ota, R. Ishihara, Y. Mizuno and T. Takeuchi, Nuc Instruments and Methods in physics Resesrch, 241, 681-684 (2005) https://doi.org/10.1016/j.nimb.2005.07.164
  12. A. I. Ektessabi, T. Kawakami, R. Ishihara, Y. Mizuno and T. Takeuchi, J of Electron Spectroscopy and Related Phenomena 137, 801-804 (2004) https://doi.org/10.1016/j.elspec.2004.02.152
  13. P. Rozan, Y. H.kuo and F .Lambein, J. Agvic. Food. Chem, 48, 716-723 (2000) https://doi.org/10.1021/jf990729v
  14. O. H. Lowry, N. J. Rose brough, A. L. Farr and R. J. Randall, J. Biochem, 193, 265-269 (1951)
  15. F. Poitrassou, J. M. Hanchar and V. Schaltegger, Chemical Geology, 191, 3-24 (2002) https://doi.org/10.1016/S0009-2541(02)00146-8
  16. M. Tiepolo, Chemical Geology, 199, 159-177 (2003) https://doi.org/10.1016/S0009-2541(03)00083-4