Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Regulation of BAD Protein by PKA, PKCδ and Phosphatases in Adult Rat Cardiac Myocytes Subjected to Oxidative Stress

  • Cieslak, Danuta (Laboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki) ;
  • Lazou, Antigone (Laboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki)
  • Received : 2007.02.20
  • Accepted : 2007.06.08
  • Published : 2007.10.31
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Abstract

$H_2O_2$, as an example of oxidative stress, induces cardiac myocyte apoptosis. Bcl-2 family proteins are key regulators of the apoptotic response while their functions can be regulated by post-translational modifications including phosphorylation, dimerization or proteolytic cleavage. In this study, we examined the role of various protein kinases in regulating total BAD protein levels in adult rat cardiac myocytes undergoing apoptosis. Stimulation with 0.1 mM $H_2O_2$, which induces apoptosis, resulted in a marked down-regulation of BAD protein, which is attributed to cleavage by caspases since it can be restored in the presence of a general caspase inhibitor. Inhibition of PKC, p38-MAPK, ERK1/2 and PI-3-K did not influence the reduced BAD protein levels observed after stimulation with $H_2O_2$. On the contrary, inhibition of PKA or specifically $PKC{\delta}$ resulted in up-regulation of BAD. Decreased caspase 3 activity was observed in $H_2O_2$ treated cells after inhibition of PKA or $PKC{\delta}$ whereas inhibition of PKA also resulted in improved cell survival. Furthermore, addition of okadaic acid to inhibit selected phosphatases resulted in enhanced BAD cleavage. These data suggest that, during oxidative stress-induced cardiac myocyte apoptosis, there is a caspase-dependent down-regulation of BAD protein, which seems to be regulated by coordinated action of PKA, $PKC{\delta}$ and phosphatases.

Keywords

References

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