Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Osteogenic Differentiation of Human Adipose-derived Stem Cells within PLGA(Poly(D,L-lactic-co-glycolic acid)) Scaffold in the Nude Mouse

누드 마우스에서 Poly(D,L-lactic-co-glycolic acid) (PLGA) 지지체 내 인체 지방줄기세포의 골성분화

  • Yoo, Gyeol (Department of Plastic Surgery, College of Medicine, The Catholic University of Korea) ;
  • Cho, Sung Don (Yedam Plastic Clinic) ;
  • Byeon, Jun Hee (Department of Plastic Surgery, College of Medicine, The Catholic University of Korea) ;
  • Rhie, Jong Won (Department of Plastic Surgery, College of Medicine, The Catholic University of Korea)
  • 유결 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 조성돈 (예담 성형외과) ;
  • 변준희 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 이종원 (가톨릭대학교 의과대학 성형외과학교실)
  • Received : 2006.07.20
  • Published : 2007.03.10
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Abstract

Purpose: The object of this study was to evaluate the development of continuous osteogenic differentiation and bone formation after the subcutaneous implantation of the tissue-engineered bone, in vitro. Methods: Human adipose-derived stem cells were obtained by proteolytic digestion of liposuction aspirates. Adipose-derived stem cells were seeded in PLGA scaffolds after being labeled with PKH26 and cultured in osteogenic differentiation media for 1 month. The PLGA scaffolds with osteogenic stimulated adipose-derived stem cells were implanted in subcutaneous layer of four nude mice. Osteogenesis was assessed by RT-PCR for mRNA of osteopontin and bone sialoprotein(BSP), and immunohistochemistry for osteocalcin, and von Kossa staining for calcification of extracellular matrix at 1 and 2 months. Results: Implanted PLGA scaffold with adipose-derived stem cells were well vascularized, and PLGA scaffolds degraded and were substituted by host tissues. The mRNA of osteopontin and BSP was detected by RT-PCR in both osteogenic stimulation group and also osteocalcin was detected by immunohistochemistry at osteogenic stimulation 1 and 2 months, but no calcified extracellular deposit in von Kossa stain was found in all groups. Conclusion: In vivo, it could also maintain the characteristics of osteogenic differentiation that adipose-derived stem cells within PLGA scaffold after stimulation of osteogenic differentiation in vitro, but there were not normal bone formation in subcutaneous area. Another important factor to consider is in vivo, heterologous environment would have negative effect on bone formation as.[p1]

Keywords

Acknowledgement

Supported by : 가톨릭대학교 성모병원

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