Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of BoLA-DRB3.2 Alleles in Hanwoo (Korean cattle) by Sequence Based Typing (SBT)

  • Jeong, H.J. (Division of Animal Science and Resources, Chungnam National University) ;
  • Bhuiyan, M.S.A. (Division of Animal Science and Resources, Chungnam National University) ;
  • Lee, J.S. (Division of Animal Science and Resources, Chungnam National University) ;
  • Yu, S.L. (Division of Animal Science and Resources, Chungnam National University) ;
  • Sang, B.C. (Division of Animal Science and Resources, Chungnam National University) ;
  • Yoon, D. (Division of Animal Genomics & Bioinformatics, National Institute of Animal Science) ;
  • Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, J.H. (Division of Animal Science and Resources, Chungnam National University)
  • Received : 2007.04.03
  • Accepted : 2007.07.22
  • Published : 2007.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

A study was conducted with 70 Hanwoo (Korean cattle) for genotyping bovine leukocyte antigen (BoLA)-DRB3.2 gene by using the polymerase chain reaction (PCR) and sequence based typing (SBT). Two-step PCR was carried out for amplifying a 284 bp fragment of the target gene and the PCR products were digested with three restriction enzymes namely RsaI, BstYI and HaeIII. Seventeen alleles were detected with frequencies ranging from 1.43 to 18.57% and one (x'aa) of these alleles was identified as a new allele that has not been reported before. The frequency of the new x'aa allele identified in this breed was 12.86%. In addition, the seven most frequently observed alleles (DRB3.2 *10, *15, *16, *26, *27, *54 and x'aa) accounted for 74.28% of the alleles in this population. The phylogenetic tree showed that the BoLA-DRB3.2 allele sequences of Hanwoo were shared with other Bos taurus breeds and no specific clade for Hanwoo was identified. It indicates high heterogeneity of the BoLA-DRB3 gene in this population and may give some ideas for breeding animals having better disease resistance.

Keywords

References

  1. Aida, Y., M. Niimi, M. Asahina, K. Okada, Y. Nakai and K. Ogimoto. 1995. Identification of a new bovine MHC class II DRB allele by nucleotide sequencing and an analysis of phylogenetic relationships. Biochem. Biophys. Res. Commu. 209:981-988. https://doi.org/10.1006/bbrc.1995.1594
  2. Aldridge, B. M., S. M. McGuirk, R. J. Clark, L. A. Knapp, D. I. Watkins and D. P. Lunn. 1998. Denaturing gradient gel electrophoresis: a rapid method for differentiating BoLA DRB3 alleles. Anim. Genet. 29:389-394. https://doi.org/10.1046/j.1365-2052.1998.295361.x
  3. Beever, J. E., P. D. George, R. L. Fernando, C. J. Stormont and H. A. Lewin. 1990. Associations between genetic markers and growth and carcass traits in a paternal half-sib family of Angus cattle. J. Anim. Sci. 68:337-344. https://doi.org/10.2527/1990.682337x
  4. Bodmer, J. G., S. G. E. Marsh, E. D. Albert, W. F. Bodmer, R. E. Bontrop, D. Charron, B. Dupont, H. A. Erlich, R. Fauchet, B. Mach, W. R. Mayr, P. Parham, T Sasazuki, G. M. T. Schreuder, J. L. Strominger, A. Svejgaard and P. I.Terasaki. 1996. Nomenclature for factors of the HLA system. Europ. J. Immunogenet. 24:105-151.
  5. Davies, C. J. and D. F. Antczak. 1991. Production and characterization of alloantisera specific for bovine class II major histocompatibility complex antigens. Anim. Genet. 22:417-434. https://doi.org/10.1111/j.1365-2052.1991.tb00701.x
  6. Davies, C. J., L. Andersson, I. Joosten, P. Mariani, L. C. Gasbarre and E. J. Hensen. 1992. Characterization of bovine MHC class II polymorphism using three typing methods: Serology, RFLP and IEF. Euro. J. Immunogenet. 19:253-262. https://doi.org/10.1111/j.1744-313X.1992.tb00068.x
  7. Davies, C. J., L. Andersson, S. Mikko, S. A. Ellis, E. J. Hensen, H. A. Lewin, N. E. Muggli-Cockett, J. J. van der Poel and G. C. Russell. 1997. Nomenclature for factors of the BoLA system, 1996: report of the ISAG BoLA Nomenclature Committee. Anim. Genet. 28:159-246. https://doi.org/10.1111/j.1365-2052.1997.00106.x
  8. Dietz, A. B., J. C. Detilleux, A. E. Freeman, D. H. Kelly, J. R. Stabel and M. E. Kehrli.1997. Genetic association of bovine lymphocyte antigen DRB3 alleles with immunological traits of Holstein cattle. J. Dairy Sci. 80:400-405. https://doi.org/10.3168/jds.S0022-0302(97)75950-2
  9. Ellis, S. A. and K. T. Ballingall. 1999. Cattle MHC: evolution in action? Immunol. Rev. 167:159-168. https://doi.org/10.1111/j.1600-065X.1999.tb01389.x
  10. Glass, E. J., R. A. Oliver, J. L. W. Williams and P. Millar. 1992. Alloreactive T-cell recognition of bovine major histocompatibility complex class II products defined by onedimensional isoelectric focusing. Anim. Genet. 23:97-111.
  11. Gelhaus, A., L. Schnittger, D. Mehlitz, R. D. Horstmann and C. G. Meyer. 1995. Sequence and PCR-RFLP analysis of 14 novel BoLA-DRB3 alleles. Anim. Genet. 26:147-153. https://doi.org/10.1111/j.1365-2052.1995.tb03154.x
  12. Germain, R. N. 1994. MHC-dependent antigen processing and peptide presentation: providing ligands for T lymphocyte activation. Cell 76:287-299. https://doi.org/10.1016/0092-8674(94)90336-0
  13. Gilliespie, B. E., B. M. Jayarao, H. H. Dowlen and S. P. Oliver. 1999. Analysis and frequency of bovine lymphocyte antigen DRB3.2 alleles in Jersey cows. J. Dairy Sci. 82:2049-2053. https://doi.org/10.3168/jds.S0022-0302(99)75443-3
  14. Kumar, S., K. Tamura and M. Nei. 2004. MEGA 3.1: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Br. Bioinfor. 5:150-163. https://doi.org/10.1093/bib/5.2.150
  15. Ledwidge, S. A., B. A. Mallard, J. P. Gibson, G. B. Jansen and Z. I. Jiang. 2001. Multi-primer target PCR for rapid identification of bovine DRB3 alleles. Anim. Genet. 32:219-221. https://doi.org/10.1046/j.1365-2052.2001.00761.x
  16. Maillard, J. C., C. Renard, P. Chardon, I. Chantal and A. Bensaid. 1999. Characterization of 18 new BoLA-DRB3 alleles. Anim. Genet. 30:200-203. https://doi.org/10.1046/j.1365-2052.1999.00446.x
  17. Miller, M. M., L. D. Bacon, K. Hala, H. D. Hunt, S. J. Ewald, J. Kaufman, R. Zoorob and W. E. Briles. 2004 Nomenclature for the chicken major histocompatibility (B and Y) complex. Immunogenetics. 56:261-279.
  18. Miltiadou, D., A. S. Law and G. C. Russel. 2003. Establishment of a sequence-based typing system for BoLA-DRB3 exon 2. Tissue Antigens. 62:55-65. https://doi.org/10.1034/j.1399-0039.2003.00080.x
  19. Moon, J. O., Y. S. Park, K. O. Lee and B. H. Ahn. 1997. DNA polymorphism analysis of BoLA DRB3 gene between Hanwoo and Holstein in Korea. Kor. J. Genet. 19:343.
  20. Mosafer, J. and M. R. Nassiry. 2005. Identification of bovine lymphocyte antigen DRB3.2 alleles in Iranian Golpayegani cattle by DNA test. Asian-Aust. J. Anim. Sci. 18:1691-1695. https://doi.org/10.5713/ajas.2005.1691
  21. Park, C. A., H. C. Hines, D. R. Monke and W. T. Threlfall. 1993. Association between the bovine major histocompatibility complex and chronic posterior spinal paresis - a form of ankylosing spondylitis in Holstein bulls. Anim. Genet. 24:53-58. https://doi.org/10.1111/j.1365-2052.1993.tb00919.x
  22. Pashmi, M., S. A. Ghorashi, A. R. Salehi, M. Moini, A. Javanmard, S. Qanbari and S. Yadranji-Aghdam. 2006. Polymorphism of bovine lymphocyte antigen DRB3.2 alleles in Iranian native Sarabi cows. Asian-Aust. J. Anim. Sci. 19:775-778. https://doi.org/10.5713/ajas.2006.775
  23. Paswan, C., B. Bhushan, B. N. Patra, P. Kumar, A. Sharma, S. Dandapat, A. K. S. Tomar and T. Dutt. 2005. Characterization of MHC DRB3.2 alleles of crossbred cattle by polymerase chain reaction-restriction fragment length polymorphism. Asian-Aust. J. Anim. Sci. 18:1226-1230. https://doi.org/10.5713/ajas.2005.1226
  24. Russell, G. C., C. J. Davies, L. Andersson, S. A. Ellis, E. J. Hensen, H. A. Lewin, S. Mikko, N. E. Muggli-Cockett and J. J. van der Poel. 1997. BoLA class II nucleotide sequences. Anim. Genet. 28:169-180. https://doi.org/10.1111/j.1365-2052.1997.00107.x
  25. Sharif, S., B. A. Mallard, B. N. Wilkie, J. M. Sargeant, H. M. Scott, J. C. M. Dekkers and K. E. Leslie. 1998a. Associations of the bovine major histocompatibility complex DRB3 (BoLADRB3) alleles with occurrence of disease and milk somatic cell score in Canadian dairy cattle. Anim. Genet. 29:185-193. https://doi.org/10.1111/j.1365-2052.1998.00318.x
  26. Sharif, S., B. A. Mallard, B. N. Wilkie, J. M. Sargeant, H. M. Scott, J. C. M. Dekkers and K. E. Leslie. 1998b. Associations of the bovine major histocompatibility complex DRB3 with production traits in Canadian dairy cattle. Anim. Genet. 30:157-160. https://doi.org/10.1046/j.1365-2052.1999.00459.x
  27. Sitte, K., I. J. East, M. F. Lavin and E. C. Jazwinska. 1995. Identification and characterization of new BoLA DRB3 alleles by heteroduplex analysis and direct sequencing. Anim. Genet. 26:413-417.
  28. Sitte, K., I. J. East and E. C. Jazwinska. 1996. Detection of a common BoLA DRB3 deletion by sequence-specific oligonucleotide typing. Anim. Genet. 27:271-273. https://doi.org/10.1111/j.1365-2052.1996.tb00488.x
  29. Smith, D. M., J. J. Lunney, C. S. Ho, G. W. Martens, A. Ando, J. H. Lee, L. Schook, C. Renard and P. Chardon 2005. Nomenclature for factors of the swine leukocyte antigen class II system. Tissue Antigens. 66:623-639. https://doi.org/10.1111/j.1399-0039.2005.00492.x
  30. Starkenburg, R. J., L. B. Hansen, M. E. Kehrli, Jr. and H. Chester- Jones. 1997. Frequencies and effects of alternative DRB3.2 alleles of bovine lymphocyte antigen for Holsteins in milk secretion and control lines. J. Dairy Sci. 80:3411-3419. https://doi.org/10.3168/jds.S0022-0302(97)76316-1
  31. Takeshima, S., M. Ikegami, M. Morita, Y. Nakai and Y. Aida. 2001. Identification of new cattle BoLA-DRB3 alleles by sequencebased typing. Immunogenetics 53:74-81. https://doi.org/10.1007/s002510000293
  32. Takeshima, S., Y. Nakai, M. Ohta and Y. Aida. 2002. Characterization of DRB3 alleles in the MHC of Japanese shorthorn cattle by polymerase chain reaction-sequence based typing. J. Dairy Sci. 85:1630-1632. https://doi.org/10.3168/jds.S0022-0302(02)74234-3
  33. Takeshima, S., N. Saitou, M. Morita, H. Inoko and Y. Aida. 2003. The diversity of bovine MHC class II DRB3 genes in Japanese Black, Japanese Shorthorn, Jersey and Holstein cattle in Japan. Gene 316:111-118. https://doi.org/10.1016/S0378-1119(03)00744-3
  34. Tamura, K. and M. Nei. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 10:512-526.
  35. Teale, C., J. F. Morrison, R. L. Page and S. B. Pearson. 1991. Dose response to inhaled salbutamol in chronic obstructive airways disease. Postgrad. Med. J. 67:754-756. https://doi.org/10.1136/pgmj.67.790.754
  36. Thompson, J. D., D. G. Higgins and T. J. Gibson. 1994. CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680. https://doi.org/10.1093/nar/22.22.4673
  37. Townsend, A. and H. Bodmer. 1989. Antigen recognition by class I-restricted T lymphocytes. Annu. Rev. Immunol. 7:601-624. https://doi.org/10.1146/annurev.iy.07.040189.003125
  38. Van Eijk, M. J., J. A. Stewart-Haynes and H. A. Lewin. 1992. Extensive polymorphism of the BoLA DRB3 gene distinguished by PCR-RFLP. Anim. Genet. 23:483-496. https://doi.org/10.1111/j.1365-2052.1992.tb00168.x
  39. Watkins, D. I., J. A. Shadduck, C. E. Rudd, M. E. Stone, H. A. Lewin and N. L. Letvin. 1989. Isoelectric focusing of bovine major histocompatibility complex class II molecules. Eur. J. Immunol. 19:567-570. https://doi.org/10.1002/eji.1830190326

Cited by

  1. The Diversity of BoLA-DRB3 Gene in Iranian Native Cattle vol.21, pp.4, 2007, https://doi.org/10.5713/ajas.2008.70054
  2. Genetic assessment of BoLA-DRB3 polymorphisms by comparing Bangladesh, Ethiopian, and Korean cattle vol.63, pp.2, 2007, https://doi.org/10.5187/jast.2021.e37