Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Birth of puppies after intrauterine and intratubal insemination with frozen-thawed canine semen

  • Kim, Hye-Jin (Haemaru Small Animal Referral Hospital) ;
  • Oh, Hyun-Ju (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Jang, Goo (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Min-Kyu (Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Seoul National University)
  • Published : 20070300
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Abstract

The present study was performed to assess the fertility of frozen-thawed dog semen prepared by freezing with 6% glycerol and thawing at 70ºC for 8 sec, and to evaluate the least number of post-thaw spermatozoa necessary to achieve pregnancy by intrauterine or intratubal artificial insemination. It was found that the pregnancy rate of intrauterine artificial insemination was 100% using 6% glycerol buffer and thawing at 70ºC for 8 sec with 5 × 107 spermatozoa. Even though the pregnancy rate (80%) and the whelping rate (24.5%) in the 5 × 106 spermatozoa inseminated group were lower than those of the 5 × 107 spermatozoa group, conception was confirmed with 5 × 106 spermatozoa. Although the pregnancy rate of intratubal insemination was low (20%) with 4 × 106 spermatozoa, this study is the first report to show the pregnancy rate of intratubal insemination with frozen-thawed ejaculated canine semen. In order to improve the pregnancy rate with intratubal insemination of canine spermatozoa, it is necessary to investigate the optimal insemination site of the uterine tube, the appropriate number of sperm, and the direct effect of buffer on oocytes.

Keywords

Acknowledgement

This study was financially supported by KOSEF (grant #M10625030005-06N250300510). The authors are grateful for a graduate fellowship provided by the Ministry of Education through the BK21 Program.

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