Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Redifferentiation of Dedifferentiated Chondrocytes on Chitosan Membranes and Involvement of PKCα and P38 MAP Kinase

  • Lee, Yoon Ae (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Kang, Shin-Sung (Daegu Center, Korea Basic Science Institute) ;
  • Baek, Suk-Hwan (Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University) ;
  • Jung, Jae-Chang (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Jin, Eun Jung (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Tak, Eun Nam (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Sonn, Jong Kyung (Department of Biology, College of Natural Sciences, Kyungpook National University)
  • Received : 2006.06.01
  • Accepted : 2007.04.19
  • Published : 2007.08.31
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Abstract

To investigate the effects of chitosan on the redifferentiation of dedifferentiated chondrocytes, we used chondrocytes obtained from a micromass culture system. Micromass cultures of chick wing bud mesenchymal cells yielded differentiated chondrocytes, but these dedifferentiated during serial monolayer subculture. When the dedifferentiated chondrocytes were cultured on chitosan membranes they regained the phenotype of differentiated chondrocytes. Expression of protein kinase $C{\alpha}$ ($PKC{\alpha}$) increased during chondrogenesis, decreased during dedifferentiation, and increased again during redifferentiation. Treatment of the cultures with phorbol 12-myristate 13-acetate (PMA) inhibited redifferentiation and down-regulated $PKC{\alpha}$. In addition, the expression of p38 mitogen-activated protein (MAP) kinase increased during redifferentiation, and its inhibition suppressed redifferentiation. These findings establish a culture system for producing chondrocytes, point to a new role of chitosan in the redifferentiation of dedifferentiated chondrocytes, and show that $PKC{\alpha}$ and p38 MAP kinase activities are required for chondrocyte redifferentiation in this model system.

Keywords

Acknowledgement

Supported by : Korea Science and Engineering Foundation (KOSEF)

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