민어, Miichtys miiuy (Basilewsky)의 성장과 연관된 생식소 발달

Gonad Ontogeny in Relation to Somatic Growth in the Brown Croaker Miichthys miiuy (Basilewsky)

  • 박인석 (한국해양대학교 해양환경.생명과학부) ;
  • 설동원 (한국해양대학교 해양환경.생명과학부) ;
  • 임수연 (한국해양대학교 해양환경.생명과학부) ;
  • 박민욱 (한국해양대학교 해양환경.생명과학부) ;
  • 허우준 (한국해양대학교 해양환경.생명과학부) ;
  • 조성환 (한국해양대학교 해양환경.생명과학부) ;
  • 송영채 (한국해양대학교 건설.환경공학부) ;
  • 김재수 (한국해양대학교 조선해양시스템공학부) ;
  • 조효제 (한국해양대학교 조선해양시스템공학부) ;
  • 노충환 (한국해양연구원 자원연구본부) ;
  • 최희정 (한국해양연구원 자원연구본부)
  • Park, In-Seok (Division of Marine Environment and Bioscience, College of Ocean Science and Technology, Korea Maritime University) ;
  • Seol, Dong-Won (Division of Marine Environment and Bioscience, College of Ocean Science and Technology, Korea Maritime University) ;
  • Im, Soo-Yeon (Division of Marine Environment and Bioscience, College of Ocean Science and Technology, Korea Maritime University) ;
  • Park, Min Ouk (Division of Marine Environment and Bioscience, College of Ocean Science and Technology, Korea Maritime University) ;
  • Hur, Woo June (Division of Marine Environment and Bioscience, College of Ocean Science and Technology, Korea Maritime University) ;
  • Cho, Sung Woan (Division of Marine Environment and Bioscience, College of Ocean Science and Technology, Korea Maritime University) ;
  • Song, Young-Chae (Division of Civil and Environmental System Engineering, College of Engineering, Korea Maritime University) ;
  • Kim, Jea-Soo (Division of Ocean System Engineering, College of Ocean Science and Technology, Korea Maritime University) ;
  • Jo, Hyo-Jae (Division of Ocean System Engineering, College of Ocean Science and Technology, Korea Maritime University) ;
  • Noh, Choong Hwan (Marine Resources Research Division, KORDI) ;
  • Choi, Hee Jung (Marine Resources Research Division, KORDI)
  • 투고 : 2007.04.13
  • 심사 : 2007.05.11
  • 발행 : 2007.06.30

초록

부화부터 부화 후 120일까지 민어, Miichthys miiuy (Basilewsky)의 성분화를 조사하였다. 시원세포는 부화 후 20일(전장 10.4 mm, 체중 0.14 g)에 출현하여 부화 후 40일(전장 19.4 mm, 체중 0.39 g)에 복강으로 이동하기 시작하였다. 부화 후 65일(전장 31.3 mm, 체중 0.93 g, $1,560D^{\circ}$ (적산온도))에 응축된 염색질 상태인 시원세포는 감수분열을 보여 난소로의 분화가 확인되었다. 부화후 120일(전장 4.60 mm, 체중 1.38 g, $2,880D^{\circ}$)에 난모세포는 주변인기 단계로 직경이 $20{\sim}40{\mu}m$로 증가하였다. 성분화후 난모세포는 크기 증가를 보인 반면, 정소는 부화 후 65일부터 증식하기 시작하였다. 부화 후 80일(전장 37.9 mm, 체중 1.39 g, $1,920D^{\circ}$)에 정소 체세포에 싸여진 정원세포 낭포의 출현과 정소 소엽 형성이 시작되었다. 부화 후 120일에 정소 소엽에는 시원세포와 정모세포가 존재하였다. 본 연구 결과 민어의 성분화 양상은 분화형자웅이체이다.

Sex differentiation of the brown croaker Miichthys miiuy (Basilewsky) is described from hatching to the 120th day post-hatching (dph) (water temperature $24^{\circ}C$). Primordial germ cells (PGCs) were observed on the 20th dph (10.4 mm total length (TL), 0.14 g body weight (BW), and began to protrude into the peritoneal cavity from the 40th dph (19.4 mm TL, 0.39 g BW). On the 65th dph (31.3 mm TL, 0.93 g BW, $1,560D^{\circ}$ (degree-days)), initial ovarian differentiation was identified by the PGCs with condensed chromatin, and their transformation into meiotic oocytes. By the 120th dph (4.60 mm TL, 1.38 g BW, $2,880D^{\circ}$), the oocytes were in the perinucleolus stage and had increased from 20 to $40{\mu}m$ in diameter. While ovaries gradually grew after sex was differentiated, testes continued to multiply from the 65th dph. On the 80th dph (37.9 mm TL, 1.39 g BW, $1,920D^{\circ}$), the beginning of testis lobule formation was indicated by the occurrence of spermatogonial cysts enveloped by somatic cells in some of the testes. On the 120th dph, the testis lobules of some of the fish contained all germ cell stages through to the spermatocytes. Therefore, the sex differentiation type of the brown croaker is identified as gonochoristic.

키워드

과제정보

연구 과제 주관 기관 : Korea Research Foundation, Ministry of Commerce, Industry and Energy

참고문헌

  1. Atz, J.W. 1964. Intersexuality in fishes. In: Armstrong CN, Marshall AJ (eds). Intersexuality in Vertebrates Including Man. Academic Press, London, pp. 145-222
  2. Choi, Y., J.H. Kim and J.Y. Park. 2002. Marine Fishes of Korea. Kyo-Hak Publ. Co., Ltd., Seoul, Korea
  3. Gompertz, B. 1925. On the nature of the function expressive of the law human mortality, and on a new mode of determining the value of life contingencies. Phil. Trans. Roy. Soc. Lond., 115 : 515-585
  4. Nagai, T., E. Yamaha and K. Arai. 2001. Histolgical differentiation of primordial germ cells in zebrafish. Zool. Sci., 18 : 215-223 https://doi.org/10.2108/zsj.18.215
  5. Nakamura, M., T. Kobayashi, X.T. Chang and Y. Nagahama. 1998. Gonadal sex differentiation in teleost fish. J. Exp. Zool., 281 : 362-372 https://doi.org/10.1002/(SICI)1097-010X(19980801)281:5<362::AID-JEZ3>3.0.CO;2-M
  6. Park, I.S., J.H. Kim, S.H. Cho and D.S. Kim. 2004. Sex differentiation and hormonal sex reversal in the brgrid catfish Psedobagrus fulvidraco (Richardson). Aquaculture, 232 : 183-193 https://doi.org/10.1016/S0044-8486(03)00481-2
  7. Romanov, A.A. and Y. Altuf'ev. 1993. Ectopic histogenesis of sexual cells of Caspian Sea sturgeons. J. Ichthyol, 33 : 140-150
  8. Timmermans, L.P.M. 1987. Early development and differentiation in fish. Sarsia., 72 : 231-339 https://doi.org/10.1080/00364827.1987.10419720
  9. von Bertalanffy, L. 1938. A quantitative theory of organic growth (Inquiries on growth laws. II). Hum. Biol., 10 : 181-213
  10. Yamamoto, T.O. 1969. Sex differentiation. In: Hoar WS, Randall DJ (eds). Fish Physiology. Academic Press, New York, pp. 117-175