Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Antioxidant Enzyme Activities and Soil Properties of Healthy and Declining Abies koreana (Wils.) in Mt. Halla

한라산 구상나무 건전개체와 쇠약개체의 항산화효소활성 및 토양특성

  • Lim, Jong-Hwan (Department of Forest Environment, Korea Forest Research Institute) ;
  • Woo, Su-Young (Department of Environmental Horticulture, University of Seoul) ;
  • Kwon, Mi Jeong (National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Kim, Young Kul (Department of Forest Environment, Korea Forest Research Institute)
  • 임종환 (국립산림과학원 산림환경부 산림생태과) ;
  • 우수영 (서울시립대학교 환경원예학과) ;
  • 권미정 (서울대학교 농업과학공동기기센터) ;
  • 김영걸 (국립산림과학원 산림환경부 산림생태과)
  • Received : 2006.08.18
  • Accepted : 2006.09.29
  • Published : 2007.03.31
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Abstract

In order to examine the differences in antioxidant enzyme activities which represent defence mechanism to stressful environments, and soil properties between healthy and declining (or unhealthy) trees, we selected three sites, Witseorum, Youngsil and Sungpanak (Jindallebat). Antioxidant enzymes including Ascorbate peroxidase (APX) and Glutathione Reductase (GR), forest soil properties including soil texture, soil pH, organic matter, total nitrogen, available phosphate, cation exchange capacity, exchangeable cation content and nutrient contents in leaves of Abies koreana (Korean fir) trees were analyzed. There were no significant differences between healthy and declining trees in GR activity. However, seasonal difference in antioxidant enzyme activity was observed. GR activity was lower in June and August than that of September. Soil chemical and physical properties of each site showed a tendency that organic content, total nitrogen content, available phosphorus, cation exchange capacity and cation content were lower at the site of declining trees than the site of healthy trees.

구상나무의 스트레스 적응능력을 보여주는 항산화효소활성, 건전개체와 쇠약한 개체의 토양의 물리화학적 특성을 알아보기 위하여 윗세오름, 영실 그리고 성판악(진달래밭)지역의 건전개체와 쇠퇴개체를 조사 분석하였다. 항산화효소활성으로는 Ascorbate peroxidase(APX)와 Glutathione Reductase(GR)를, 토양특성으로는 토성, pH, 유기물함량, 전 질소함량, 유효인산, 양이온치환용량, 양이온의 함량을 분석하였고, 구상나무 잎을 채취하여 식물체의 양분함량을 분석하였다. APX, GR 효소 활성에 있어서 건전개체와 쇠약한 개체 사이에는 큰 차이가 없었으나, 계절적으로 6월과 8월에 비해 9월의 항산화효소 활성도가 낮게 나타났다. 토양분석 결과 유기물함량, 전질소, 유효인산, 양이온치환용량, 양이온 함량이 건전개체가 있는 곳에 비해 쇠약한 개체가 있는 곳의 토양이 낮게 나타났다.

Keywords

References

  1. 강상준. 1997. 한라산 구상나무 숲의 재생에 대하여. 제17회 국제학술대회 논문집 pp.21-50
  2. 구경아,박원규,공우석. 2001. 한라산 구상나무 (Abies koreana W.)의 연륜연대학적 연구 -기후변화에 따른 생장변동 분석-. 한국생태학회지 24: 281-288
  3. 김갑태,추갑철,엄태원. 1997. 지리산 천왕봉-덕평봉 지역의 삼림군집구조에 관한 연구-구상나무림-. 한국임학회지 86: 146-157
  4. 김은식,김상돈,차윤정. 1994. 환경변화와 고산지대 수목생장 쇠퇴현상과의 상관성 해석. 한국과학재단 연구보고서 KOSEF 921-1500-018-2,89pp
  5. 김문홍,남정현. 1985. 한라산 구상나무림의 식물사회학적 연구. 한라산 학술조사보고서, pp.299-309
  6. 송종석. 1991. 한라산 구상나무림의 식물사회학적 식생 단위의 재검토. 식물학회지 34: 341-347
  7. 오순자, 고정군, 김응식, 오문유, 고석찬. 2001. 한라산 구상나무 잎의 엽록소형광의 일변화와 계절적 변화. Korean Journal of Environmental Biology 19: 43-48
  8. 이윤원, 홍성천. 1995. 구상나무林의 군락생태학적 연구, 한국임학회지 84(2): 247-257
  9. 이창석, 조현제. 1993. 가야산 구상나무 군락의 구조 및 동태. 한국생태학회지 16: 75-91
  10. 이창복. 1970. 구상나무와 새로 발견된 품종. 한국임학회지 10: 5-6
  11. 임양재, 김정언, 이남주, 이용범, 백광수. 1990. 한라산 국립공원 식물군집의 식물사회학적 분류. 한국생태학회지 13: 101-130
  12. 차종환. 1969. 한라산 식물의 수직분포. 한국식물학회지 12: 161-171
  13. Alscher R.G. and J.L. Hess. 1993. Antioxidants in higher plants. CRC Press, Boca Raton. pp. 1-174
  14. Anderson M.D., T.K. Prasad and C.R. Stewart. 1995. Changes in isozyme profiles of catalase, peroxidase, and glutathione reductase during acclimation to chilling in mesocotyls of maize seedlings. Plant Physiology 109: 1247-1257 https://doi.org/10.1104/pp.109.4.1247
  15. Asada K. 1992. Ascorbate peroxidase - a hydrogen peroxide-scavenging enzyme in plants. Plant Physiology 85: 235-241 https://doi.org/10.1111/j.1399-3054.1992.tb04728.x
  16. Bradford, M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248 https://doi.org/10.1016/0003-2697(76)90527-3
  17. Drohan, P.J., S.L. Stout and G.W. Petersen. 2002. Sugar maple (Acer saccharum Marsh.) decline during 1979-1989 in northern Pennsylvania. Forest Ecology and Management 170: 1-17 https://doi.org/10.1016/S0378-1127(01)00688-0
  18. Gustafsson, M.E.R. 1997, Raised levels of marine aerosol deposition owing to increased storm frequency; a cause of forest decline in southern Sweden? Agricultural and Forest Meteorology 84: 169-177 https://doi.org/10.1016/S0168-1923(96)02384-2
  19. Hodges, D.M., C.J. Andrews, D.A. Johnson and R.I. Hamilton, 1997, Antioxidant enzymes responses to chilling stress in differentially sensitive inbred maize lines, Journal of Experimental Botany 48: 1105-1113 https://doi.org/10.1093/jxb/48.5.1105
  20. Inze D. and M. Van Montagu, 1995, Oxidative stress in plants Curr. Opin. Biotechnol. 6: 153-158 https://doi.org/10.1016/0958-1669(95)80024-7
  21. Lidon, F.C. and F.S. Henniques. 1993, Oxygen metabo- lism in higher plant chloroplasts, Photosynth, 29: 249-279
  22. Rao, M.V., G. Paliyath and D.P. Ormrod, 1996, Ultraviolet-B and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiology 110: 125-136 https://doi.org/10.1104/pp.110.1.125
  23. Song, J.S. and S. Nakanishi. 1985, Phytosociological study of the subalpine forests on Mt. Halla of Cheju island, Korea. Japanese Journal of Ecology 35: 317-328
  24. Sprtova, M., M.V. Marek, L. Nedbal, O. Prasil and J. Kalina, 1999, Seasonal changes of photosynthetic assimilation of Norway spruce under the impact of enhanced UV-B radiation, Plant Science 142: 37-45 https://doi.org/10.1016/S0168-9452(98)00253-2
  25. Walker, M.A. and B.D. McKersie, 1993, Role of the ascorbate-glutathione antioxidant system in chilling resistance of tomato, Journal of Plant Physiology 141: 234239 https://doi.org/10.1016/S0176-1617(11)80766-2
  26. Yim, Y.J. and J.U. Kim, 1990, Review on Abies koreana community in Mt. Halla national park, Korea. Korean Journal of Ecology 13: 247-249