Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Glucose Tolerance and Insulin Response to Intravenous Glucose Load in Sheep Fed on Germinated Sorghum Grain

  • Achmadi, Joelal (Laboratory of Nutritional Biochemistry Faculty of Animal Agriculture, Diponegoro University) ;
  • Pangestu, Eko (Laboratory of Nutritional Biochemistry Faculty of Animal Agriculture, Diponegoro University) ;
  • Wahyono, Fajar (Laboratory of Nutritional Biochemistry Faculty of Animal Agriculture, Diponegoro University)
  • Received : 2006.07.06
  • Accepted : 2006.11.14
  • Published : 2007.10.01
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Abstract

The glucose tolerance and pancreatic insulin secretion response to glucose in sheep fed on germinated sorghum grain were determined using an intravenous glucose load. Twelve male Thin Tail sheep (an Indonesian native sheep, 12 months old and 14.8 kg average body weight) were divided randomly into sorghum grain-based (S), germinated sorghum grain-based (G) and maize grain-based (C) diets. Sheep were maintained at the same daily intake levels of metabolizable energy and crude protein in the diets throughout the experimental period. After two months of the experimental conditions, each diet group was subjected to an intravenous glucose load experiment in which five doses of glucose (0, 10, 20, 40 and 80 mg/kg BW) were injected to estimate the rate of glucose removal from blood and the pancreatic insulin secretion response. For each sheep and each glucose load dose, the incremental blood serum glucose and insulin concentrations above pre-injection concentration were calculated as serum glucose and insulin response areas. At all glucose doses, sheep fed on S diet had a greater (p<0.05) glucose response area compared to those of sheep fed on G and C diets. Likewise at all glucose doses, the insulin response area was smaller (p<0.05) in sheep fed on S diet than in sheep fed on G and C diets. The glucose and insulin response areas in sheep fed on G and C diets differed slightly. It was concluded that the portion of maize grain in the ruminant ration could be substituted by germinated sorghum grain.

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References

  1. Achmadi, J., T. Yanagisawa, H. Sano and Y. Terashima. 1993. Pancreatic insulin secretory response and insulin action in heat-exposed sheep given a concentrate or roughage diet. Domest. Anim. Endocrinol. 10(4):279-297. https://doi.org/10.1016/0739-7240(93)90032-7
  2. Bassett, J. M. 1975. Dietary and gastrointestinal control of hormones regulating carbohydrate metabolism in ruminants. In : (Ed. I. W. McDonald and Proc. of The IV International Symposium on Ruminants PhysiologyA. C. I. Warner). The University of New England Publishing Unit, Armidale, NSW. Australia. pp. 383-398.
  3. Bassett, J. M. 1978. Endocrine factors in the control of nutrient utilization: ruminants. Proc. Soc. 37:273-280.
  4. Cole, N. A., D. M. Hallford and R. Gallavan. 1993. Influence of a glucose load in fed or unfed lambs on blood metabolites and hormone patterns. J. Anim. Sci. 71:765-773.
  5. Fujita, T., M. Kajita, H. Sano and A. Shiga. 2006. Effects of nonprotein energy on the concentrations of plasma metabolites and insulin, and tissue responsiveness and sensitivity to insulin in goats. Asian-Aust. J. Anim. Sci. 19:1010-1018. https://doi.org/10.5713/ajas.2006.1010
  6. Fujita, T., M. Kajita and H. Sano. 2007. Effects of dietary starch and sucrose on tissue responsiveness and sensitivity to insulin in goats fed a high-concentrate diet. Asian-Aust. J. Anim. Sci. 20:385-392. https://doi.org/10.5713/ajas.2007.385
  7. Hartadi, H., S. Reksohadiprodjo, S. Lebdosukojo and A. D. Tillman. 1994. Table of Feed Composition for Indonesia. Gadjah Mada University Press, Yogyakarta.
  8. Janes, A. N., T. E. C. Weekes and D. G. Armstrong. 1985. Absorption and metabolism of glucose by the mesentricdrained viscera of sheep fed on dried-grass, maize-based diets. Br. J. Nutr. 54:449-446. https://doi.org/10.1079/BJN19850130
  9. McCann, J. P., M. B. Ullmann, M. R. Temple, T. J. Reimers and E. N. Bergman. 1986. Insulin and glucose response to glucose injection in fed and fasted obese and lean sheep. J. Nutr. 116:1287-1297. https://doi.org/10.1093/jn/116.7.1287
  10. Mitaru, B. N., R. D. Reichert and R. Blair. 1985. Protein and amino acid digestibilities for chickens of reconstituted and boiled sorghum grains varying in tannin contents. Poult. Sci. 64:101-106. https://doi.org/10.3382/ps.0640101
  11. NRC. 1985. Nutrient Requirement of Sheep. 6th Rev. Ed. National Academy of Science, Washington DC.
  12. Reichert, R. D., S. E. Fleming and D. J. Schwab. 1980. Tannin and nutritional improvement of sorghum by aerob storage on $H_2O$ or NaOH treated grain. J. Agric. Food Chem. 28:824-829. https://doi.org/10.1021/jf60230a045
  13. Sasaki, Y., H. Takahashi, H. Aso, H. Hikosaka, A. Agino and S. Oda. 1984. Insulin response and glucose tolerance following feeding in sheep. Br. J. Nutr. 52:351-358. https://doi.org/10.1079/BJN19840101
  14. Sasaki, Y. and H. Takahashi. 1980. Insulin secretion response in sheep exposed to cold. J. Physiol. (London) 306:323-335. https://doi.org/10.1113/jphysiol.1980.sp013399
  15. Sasaki, S. and Y. Watanabe. 1990. Insulin sensitivity and responsiveness to isolated ovine adipocytes. Jpn. J. Zootech. Sci. 61:549-556.
  16. Sumiati and D. M. Suci. 1996. An effort to improve the nutritive value of sorghum by high moisture storage treatment on broiler performance. Indon. J. Nutr. Feed Sci. 1(1):9-12.
  17. Trenkle, A. 1978. Relation of hormonal variations to nutritional studies and metabolism of ruminants. J. Dairy Sci. 61:281-293. https://doi.org/10.3168/jds.S0022-0302(78)83595-4
  18. Waghorn, G. C., D. S. Flux and M. J. Ulyatt. 1987. Effects of dietary protein and energy intakes on growth hormone, insulin, glucose tolerance and fatty acid synthesis in young whether sheep. Anim. Prod. 44:143-152. https://doi.org/10.1017/S0003356100028154
  19. Weekes, T. E. C., Y. Sasaki and T. Tsuda. 1983. Enhanced responsiveness to insulin in sheep exposed to cold. Am. J. Physiol. 224(7):E335-E345.
  20. Weekes, T. E. C. 1991. Hormonal control of glucose Metabolism. In: Physiological Aspects of Digestion and Metabolism in Ruminants (Ed. T. Tsuda, Y. Sasaki and R. Kawashima). Proceeding of the 7th International Symposium on Ruminant Physiology. Academic Press, San Diego. pp. 183-200.

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