Cloning and expression of cDNA for chemokine receptor 9 from Olive flounder, Paralichthys olivaceus

  • Kim, Mu-Chan (Institute of Marine Industry, The College of Marine Science, Gyeongsang National University) ;
  • An, Geun-Hee (Institute of Marine Industry, The College of Marine Science, Gyeongsang National University) ;
  • Park, Chan-Il (Institute of Marine Industry, The College of Marine Science, Gyeongsang National University)
  • Published : 20071200

Abstract

Cysteine-cysteine chemokine receptor 9 (CCR9) homologue cDNA was isolated from olive flounder leukocyte cDNA library. Olive flounder CCR9 homologue consisted of 1709 bp encoding 367amino acid residues. When compared with other known CCR peptide sequences, the most conserved region of the olive flounder CCR9 peptide is the seven transmembranes. A phylogenetic analysis based on the deduced amino acid sequence showed the homologous relationship between the olive flounder CCR9 sequence and that of Mouse CCR9. The olive flounder CCR9 gene was predominantly expressed in the Peripheral blood leukocytes (PBLs), kidney, spleen, and gills.

Keywords

References

  1. Ansel, K.M., Ngo, V.N., Hyman, P.L., Luther, S.A., Forster, R., Sedgwick, J.D., Browning, J.L, Lipp M., Cyster, J.G.: A chemokine-driven positive feedback loop organizes lymphoid follicles. Nature, 406: 309-314, 2000 https://doi.org/10.1038/35018581
  2. Baggiolini, M.: Chemokines and leukocyte traffic. Nature. 392: 565-568, 1998 https://doi.org/10.1038/33340
  3. Butcher, E.C., Williams, M., Youngman, K.. Rott, L., Briskin, M.: Lymphocyte trafficking and regional immunity. Adv, Immunol., 72: 209-253. 1999 https://doi.org/10.1016/S0065-2776(08)60022-X
  4. Campbell, J.J. and Butcher, E.C.: Chemokines in tissue-specific and microenvironment specific lymphocyte homing. Curr. Opin. Immunol., 12:336-341. 2000 https://doi.org/10.1016/S0952-7915(00)00096-0
  5. Campbell, J.J., Haraldsen, G., Pan, J., Rottman, J., Qin, S., Ponath, P., Andrew, D.P., Warnke, R., Ruffing, N., Kassam, N., Wu, L., Butcher, E.C.: The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells. Nature, 400: 776-780, 1999 https://doi.org/10.1038/23495
  6. Coillie, E.V., Damme, J.V. and Opdenakker, G.: The MCP/eotaxin subfamily of CC chemokines. Cytokine Growth Factor Rev., 10: 61-86, 1999 https://doi.org/10.1016/S1359-6101(99)00005-2
  7. Fernandez, E.J. and Lolis, E.: Structure. function. and inhibition of chemokines. Annu. Rev. Pharmacol. Toxicol., 42: 469-499, 2002 https://doi.org/10.1146/annurev.pharmtox.42.091901.115838
  8. Iwata, M., Hirakiyama, A., Eshima, Y., Kagechika, H., Kato, C. and Song, S.Y.: Retinoic acid imprints gut-homing specificity on T cells. Immunity. 21:527-538, 2004 https://doi.org/10.1016/j.immuni.2004.08.011
  9. Katagiri, T., Hirono, I. and Aoki, T.: Identification of a cDNA for medaka cyroskeletal beta actin and construction for the reverse transcriptase-polymerase chain reaction (RTPCR) primer, Fish. Sci., 63: 73-76, 1997 https://doi.org/10.2331/fishsci.63.73
  10. Kunkel, E.J., Campbell, J.J., Haraldsen, G., Pan, J., Boisvert, J., Roberts, A.I.. Ebert, E.C., Vierra, M.A., Goodman, S.B., Genovese, M.C., Wardlaw, A.J., Greenberg, H.B.. Parker, C.M., Butcher, E.C., Andrew, D.P. and Agace, W.W.: Lymphocyte CC chemokine receptor 9 and epithelial thymus-expressed chemokine (TECK) expression distinguish the small intestinal immune compartment: epithelial expression of tissue-specific chemokines as an organizing principle in regional immunity. J. Exp. Med., 192: 761-768, 2000 https://doi.org/10.1084/jem.192.5.761
  11. Lindbom, B. J., Svensson, M., Wurbel, M.A., Malissen, B., Marquez, G. and Agace, W.: Selective generation of gut tropic T cells in gut-associated lymphoid tissue (GALT): requirement for GALT dendritic cells and adjuvant. J. Exp. Med., 198: 963-969, 2003 https://doi.org/10.1084/jem.20031244
  12. Mora, J.R., Bono, M.R., Manjunath, N., Weninger, W., Cavanagh, L.L., Rosemblatt, M. and Von Andrian, U.H.: Selective imprinting of gut-homing T cells by Peyer' s patch dendritic cells. Nature, 424: 88-93, 2003 https://doi.org/10.1038/nature01726
  13. Morales, J., Homey, B., Vicari, A.P., Hudak, S., Oldham, E., Hedrick, J., Orozco, R., Copeland, N.G., Jenkins, N.A., McEvoy, L.M., Zlotnik, A.: CTACK a skin-associated chemokine that preferentially attracts skin-homing memory T cells. Proc. Natl. Acad. Sci. USA, 96: 14470-14475, 1999
  14. Nam, B.H., Yamamoto, E.. Hirono, I. and Aoki, T.: A survey of expressed genes in the leukocytes of Japanese flounder, Paralichthys olivaceus, infected with hirame rhabdovirus. Dev. Comp. Immunol., 24: 13-24, 2000 https://doi.org/10.1016/S0145-305X(99)00058-0
  15. Norment, A.M., Bogatzki, L.Y., Gantner, B.N. and Bevan, M.J.: Murine CCR9, a chemokine receptor for thymus-expressed chemokine that is up-regulated following pre-TCR signaling. J. Immunol., 164: 639-648, 2000 https://doi.org/10.4049/jimmunol.164.2.639
  16. Olaussen, R.W., Farstad, I.N., Brandtzaeg, P. and Rugtveit, J., Age-related changes in CCR9+ circulating lymphocytes: are CCR9+ naive T cells recent thymic emigrants? Scand. J. Immunol., 54: 435-439, 2001 https://doi.org/10.1046/j.1365-3083.2001.01008.x
  17. Papadakis, K.A., Prehn, J., Moreno, S.T., Cheng, L., Kouroumalis, E.A., Deem, R., Breaverman, T., Ponath, P.K., Andrew, D.P., Green, P.H., Hodge, M.R., Binder, S.W. and Targan, S.R.: CCR9-positive lymphocytes and thymus- expressed chemokine distinguish small bowel from colonic Crohn' s disease. Gastroenterology. 121: 246-254, 2001 https://doi.org/10.1053/gast.2001.27154
  18. Papadakis, K.A., Prehn, J., Nelson, V., Cheng, L., Binder, S.W., Ponath, P.D., Andrew, D.P. and Targan, S.R.: Therole of thymusexpressed chemokine and its receptor CCR9 on lymphocytes in the regional specialization of the mucosal immune system. J. Immunol., 165: 5069-5076, 2000 https://doi.org/10.4049/jimmunol.165.9.5069
  19. Rossi, D.L., Hardiman, G., Copeland, N.G., Gilbert, D.J., Jenkins, N. and Zlotnik, A.: Cloning and characterization of a new type of mouse chemokine, Genomics, 47: 163-170, 1998 https://doi.org/10.1006/geno.1997.5058
  20. Vaddi, K., Keller. M. and Newton, R.C.: The chemokine facts book. Academic Press, New York, 1997
  21. Vicari, A.P., Figueroa, D.J., Hedrick, J..A., Foster, J.S., Singh, K.P., Menon, S., Copeland, N.G., Gilbert, D.J., Jenkins, N.A., Bacon, K.B., Zlotnik, A.: TECK: a novel CC chemokine specifically expressed by thymic dendritic cells and potentially involved in T cell development. Immunity. 7: 291-301, 1997 https://doi.org/10.1016/S1074-7613(00)80531-2
  22. Willimann, K., Legler, D.F., Loetscher, M., Roos, R.S., Delgado, M.B., Clark-Lewis, I., Baggiolini, M., Moser, B.: The chemokine SLC is expressed in T cell areas of lymph nodes and mucosal lymphoid tissues and attracts activated T cells via CCR7. Eur. J. Immunol., 28:2025-2034. 1998 https://doi.org/10.1002/(SICI)1521-4141(199806)28:06<2025::AID-IMMU2025>3.0.CO;2-C
  23. Wurbel, M.A., Malissen, M., Grand, D. G., Meffre, E., Nussenzweig, M.C., Richelme, M., Carrier, A. and Malissen, B.: Mice lacking the CCR9 CC-chemokine receptor show a mild impairment of early T- and B-cell development and a reduction in T-cell receptor $\wp^{\delta+}$ gut intraepithelial lymphocytes. Blood, 98: 2626-2632, 2001 https://doi.org/10.1182/blood.V98.9.2626
  24. Wurbel, M.A., Philippe, J.M., Nguyen, C., Victorero, G., Freeman, T., Wooding, P., Miazek, A., Mattei, M.G., Malissen, M., Jordan, B.R., Malissen, B., Carrier, A. and Naquet, P.: The chemokine TECK is expressed by thymic and intestinal epithelial cells and attracts double- and single-positive thyrnocytes expressing the TECK receptor CCR9. Em.J. Immunol., 30: 262-271, 2000 https://doi.org/10.1002/1521-4141(200001)30:1<262::AID-IMMU262>3.0.CO;2-0
  25. Youn, B.S., Kim, C.H., Smith, F.O. and Broxmeyer, H.E.: TECK, an efficacious chemoattractant for human thymocytes, uses GPR-9-6/CCR9 as a specific receptor. Blood, 94: 2533-2536, 1999
  26. Zaballos, A., Gutierrez, J., Varona, R., Ardavin, C. and Marquez, G.: Cutting edge: identification of the orphan chemokine receptor GPR9-6 as CCR9, the receptor for the chemokine TECK, J. Immunol., 162: 5671-5675, 1999
  27. Zabel, B.A., Agace, W.W., Campbell, J.J., Heath, H.M., Parent, D., Roberts, A.I., Ebert, E.C., Kassam, N., Qin, S., Zovko M., LaRosa, G.J., Yang, L.L., Soler, D., Butcher, E.C., Ponath, P.D., Parker, C.M. and Andrew, D.P.: Human G protein-coupled receptor GPR-9-6/CC chemokine receptor 9 is selectively expressed on intestinal homingT lymphocytes, mucosal lymphocytes, and thymocytes and is required for thymus-expressed chemokine-mediated chemotaxis. J. Exp. Med. 190: 1241-1256, 1999 https://doi.org/10.1084/jem.190.1.9