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Detection of Mendelian and Parent-of-origin Quantitative Trait Loci in a Cross between Korean Native Pig and Landrace I. Growth and Body Composition Traits

  • Kim, E.H. (School of Biotechnology, Yeungnam University) ;
  • Choi, B.H. (Animal Genomics and Bioinformatics Division, National Livestock Research Institute) ;
  • Kim, K.S. (Department of Animal Science, Chungbuk National University) ;
  • Lee, C.K. (Division of Biotechnology and Genetic Engineering, Korea University) ;
  • Cho, B.W. (Department of Animal Science, Busan National University) ;
  • Kim, T.-H. (Animal Genomics and Bioinformatics Division, National Livestock Research Institute) ;
  • Kim, J.-J. (School of Biotechnology, Yeungnam University)
  • 투고 : 2006.09.04
  • 심사 : 2006.11.27
  • 발행 : 2007.05.01

초록

This study was conducted to detect quantitative trait loci (QTL) affecting growth and body composition in an $F_2$ reference population of Korean native pig and Landrace crossbreds. The three-generation mapping population was generated with 411 progeny from 38 $F_2$ full-sib families, and 133 genetic markers were used to produce a sex-average map of the 18 autosomes. The data set was analyzed using least squares Mendelian and parent-of-origin interval-mapping models. Lack-of-fit tests between the models were used to characterize QTL for mode of expressions. A total of 8 (39) QTL were detected at the 5% genome (chromosome)-wise level for the 17 analyzed traits. Of the 47 QTL detected, 21 QTL were classified as Mendelian expressed, 13 QTL as paternally expressed, 6 QTL as maternally expressed, and 7 QTL as partially expressed. Of the detected QTL at 5% genome-wise level, two QTL had Mendelian mode of inheritance on SSC6 and SSC9 for backfat thickness and bone weight, respectively, two QTL were maternally expressed for leather weight and front leg weight on SSC6 and SSC12, respectively, one QTL was paternally expressed for birth weight on SSC4, and three QTL were partially expressed for hot carcass weight and rear leg weight on SSC6, and bone weight on SSC13. Many of the Mendelian QTL had a dominant (complete or overdominant) mode of gene action, and only a few of the QTL were primarily additive, which reflects that heterosis for growth is appreciable in a cross between Korean native pig and Landrace. Our results indicate that alternate breed alleles of growth and body composition QTL are segregating between the two breeds, which could be utilized for genetic improvement of growth via marker-assisted selection.

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참고문헌

  1. Bidanel, J., D. Milan, N. Iannuccelli, Y. Amigues, M. Boscher, F. Bourgeois, J. Caritez, J. Gruand, P. Le Roy, H. Lagant, R. Quintanilla, C. Renard, J. Gellin, L. Ollivier and C. Chevalet. 2001. Detection of quantitative trait loci for growth and fatness in pigs. Genet. Sel. Evol. 33:289-309. https://doi.org/10.1186/1297-9686-33-3-289
  2. Bidanel, J. P. and M. Rothschild. 2002. Current status of quantitative trait locus mapping in pigs. Pig News and Information 23(2):39N-53N.
  3. Cameron, N. D. 1994. The value of pig selection experiments. Proceedings of the 5th World Congress on Genetics Applied to Livestock Production 19:41-48.
  4. Choi, B. H., J. S. Lee, G. W. Jang, H. Y. Lee, J. W. Lee, K. T. Lee, H. Y. Chung, H. S. Park, S. J. Oh, S. S. Sun, K. H. Myung, I. C. Cheong and T. H. Kim. 2006. Mapping of the porcine Calpastatin gene and association study of its variance with economic traits in pigs. Asian-Aust. J. Anim. Sci. 19:1085-1089. https://doi.org/10.5713/ajas.2006.1085
  5. Choy, Y. H., G. J. Jeon, T. K. Kim, B. H. Choi and H. W. Chung. 2002a. Ear type and coat color on growth performances of crossbred pigs. Asian-Aust. J. Anim. Sci. 15:1178-1181. https://doi.org/10.5713/ajas.2002.1178
  6. Choy, Y. H., G. J. Jeon, T. K. Kim, B. H. Choi, I. C. Cheong, H. K. Lee, K. S. Seo, S. D. Kim, Y. I. Park and H. W. Chung. 2002b. Genetic analyses of carcass characteristics in crossbred pigs: cross between Landrace sows and Korean wild boars. Asian-Aust. J. Anim. Sci. 15:1080-1084. https://doi.org/10.5713/ajas.2002.1080
  7. De Koning, D. J., L. L. G. Janss, A. P. Rattink, P. A. M. van Oers, B. J. de Vries, M. A. M. Groenen, J. J. van der Poel, P. N. de Groot, E. W. Brascamp and J. A. M. van Arendonk. 1999. Detection of quantitative trait loci for backfat thickness and intramuscular fat content in pigs (Sus scrofa). Genet. 152:1679-1690.
  8. De Koning, D. J., A. P. Rattink, B. Harlizius, J. A. M. van Arendonk, E. W. Brascamp and M. A. M. Groenen. 2000. Genome-wide scan for body composition in pigs reveals important role of imprinting. Proc. Natl. Acad. Sci. USA 97:7947-7950. https://doi.org/10.1073/pnas.140216397
  9. Edwards, D. B., R. O. Bates and W. N. Osburn. 2003. Evaluation of Duroc- vs. Pietrain-sired pigs for carcass and meat quality measures. J. Anim. Sci. 81:1895-1899. https://doi.org/10.2527/2003.8181895x
  10. Green, P., K. Fallis and S. Crooks. 1994. Documentation for CRIMAP version 2.4, Washington University School of Medicine, St. Louis, MO.
  11. Haley, C. S., S. A. Knott and J.-M. Elsen. 1994. Mapping quantitative trait loci in crosses between outbred lines using least squares. Genet. 136:1195-1207.
  12. Kim, J.-J., M. F. Rothschild, J. Beever, S. Rodriguez-Zas and J. C. M. Dekkers. 2005a. Joint analysis of two breed cross populations in pigs to improve detection and characterization of quantitative trait loci. J. Anim. Sci. 83:1229-1240. https://doi.org/10.2527/2005.8361229x
  13. Kim, J.-J. H. Zhao, H. Thomsen, M. F. Rothschild and J. C. M. Dekkers. 2005b. Combined line-cross and half-sib QTL analysis of crosses between outbred lines. Genet. Res. 85:235-248. https://doi.org/10.1017/S0016672305007597
  14. Kim, T. H., K. S. Kim, B. H. Choi, D. H. Yoon, G. W. Jang, K. T. Lee, H. Y. Chung, H. Y. Lee, H. S. Park and J. W. Lee. 2005c. Genetic structure of pig breeds from Korea and China using microsattellite loci analysis. J. Anim. Sci. 83:2255-2263. https://doi.org/10.2527/2005.83102255x
  15. Knott, S. A., L. Marklund, C. S. Haley, K. Andersson, W. Davis, H. Ellegren, M. Fredholm, I. Hansson, B. Hoyheim, K. Lundstrom, M. Moller and L. Andersson. 1998. Multiple marker mapping of quantitative trait loci in a cross between outbred wild boar and Large White pigs. Genet. 149:1069-1080.
  16. McElroy, J. P., J.-J. Kim, D. E. Harry, S. R. Brown, J. C. M. Dekkers and S. J. Lamont. 2006. Identification of trait loci affecting white meat parentage and other growth and carcass traits in commercial broiler chickens. Poult. Sci. 85:593-605. https://doi.org/10.1093/ps/85.4.593
  17. McLaren, D. G., D. S. Buchanan and R. K. Johnson. 1987. Growth performance for four breeds of swine: crossbred females and purebred and crossbred boars. J. Anim. Sci. 64:99-108. https://doi.org/10.2527/jas1987.64199x
  18. Moore, T. and D. Haig. 1991. Genomic imprinting in mammalian development: A parental tug-of-war. Trends Genet. 7:45-49. https://doi.org/10.1016/0168-9525(91)90230-N
  19. Ovilo, C., A. Oliver, J. L. Noguera, A. Clop, C. Barragan, L. Varona, C. Rodriguez, M. Toro, A. Sanchez, M. Perez-Enciso and L. Silio. 2002. Test for positional candidate genes for body composition on pig chromosome 6. Genet. Sel. Evol. 34:465-79. https://doi.org/10.1186/1297-9686-34-4-465
  20. Rohrer, G. and J. Keele. 1998. Identification of quantitative trait loci affecting carcass composition in swine. I. Fat deposition trait. J. Anim. Sci. 76:2247-2254. https://doi.org/10.2527/1998.7692247x
  21. Sato S, Y. Oyamada, K. Atsuji, T. Nade, S. Sato, E. Kobayashi, T. Mitsuhashi, K. Nirasawa, A. Komatsuda, Y. Saito, S. Terai, T. Hayashi and Y. Sugimoto. 2003. Quantitative trait loci analysis for growth and carcass traits in a Meishan$\times$Duroc F2 resource population. J. Anim. Sci. 81:2938-2949. https://doi.org/10.2527/2003.81122938x
  22. Su Y.-H., M. A. Baoyu and Y.-Z. Xiong. 2004. Genetic location of body composition traits in pigs. Hereditas (Beijing) 26(2):163-166.
  23. Thomsen, H., H. K. Lee, M. F. Rothschild, M. Malek and J. C. M. Dekkers. 2004. Characterization of quantitative trait loci for growth and meat quality in a cross between commercial breeds of swine. J. Anim. Sci. 82:2213-2228. https://doi.org/10.2527/2004.8282213x
  24. Van Laere, A. S., M. Nguyen, M. Braunschweig, C. Nezer, C. Collette, L. Moreau, A. L. Archibald, C. S. Haley, N. Buys, M. Tally, G. Andersson, M. Georges and L. Andersson. 2003. A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature 425:832-836. https://doi.org/10.1038/nature02064
  25. Walling, G. A., P. M. Visscher, L. Andersson, M. F. Rothschild, L. Wang, G. Moser, M. A. M. Groenen, J.-P. Bidanel, S. Cepica, A. L. Archibald, H. Geldermann, D. J. de Koning, D. Milan and C. S. Haley. 2000. Combined analyses of data from quantitative trait loci mapping studies: chromosome 4 effects on porcine growth and fatness. Genet. 155:1369-1378.
  26. Yue, G., A. Stratil, M. Kopecny, D. Schroffelova, J. Schroffel Jr., J. Hojny, S Cepica, R. Davoli, P. Zambonelli, C. Brunsch, I. Sternstein, G. Moser, H. Bartenschlager, G. Reiner and H. Geldermann. 2003. Linkage and QTL mapping for Sus scrofa chromosome 6. J. Anim. Breed. Genet. 120(Suppl. 1):45-55. https://doi.org/10.1046/j.0931-2668.2003.00423.x

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