Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Diversity of swine Bordetella bronchiseptica isolates evaluated by RAPD analysis and PFGE

  • Shin, Eun-Kyung (South Branch, Gangwon Veterinary Service) ;
  • Seo, Yeon-Soo (School of Veterinary Medicine, Kangwon National University) ;
  • Han, Jeong-Hee (School of Veterinary Medicine, Kangwon National University) ;
  • Hahn, Tae-Wook (School of Veterinary Medicine, Kangwon National University)
  • Published : 20070300
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Abstract

The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides for their discriminatory abilities. The OPA-07, OPA-08 and OPA-18 primers yielded 10, 10, and 6 distinct fingerprint patterns, respectively. The most common identical RAPD pattern was produced by OPA- 07 which was shared by 32 isolates (71.1%), the pattern produced by OPA-08 was shared by 26 isolates (57.8%), and the pattern produced by OPA-18 was shared by 40 isolates (88.9%). The RAPD patterns of the vaccine strain and the 3 reference strains did not match any of the patterns produced by the field isolates when primers OPA-07 and OPA-08 were used. PFGE using the restriction endonuclease XbaI produced a total of 15 patterns consisting of 4 PFGE types (A, B, B1 and C, differing by ≥ 4 bands) and 11 A subtypes (differing by ≤ 3 bands). Most of the field isolates exhibited identical type A and B patterns, suggesting that they were related. The vaccine strain and the three reference strains showed different PFGE patterns as compared to the identical type A strains.

Keywords

Acknowledgement

This work supported by a Korea Research Foundation Grant funded by the Korean Government (MOEHRD) [R05-2003-000-11001-0].

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