DOI QR코드

DOI QR Code

Effect of Levels of Supplementation of Concentrate Containing High Levels of Cassava Chip on Rumen Ecology, Microbial N Supply and Digestibility of Nutrients in Beef Cattle

  • Wanapat, M. (Tropical Feed Resources Research and Development Center (TROFREC), Faculty of Agriculture, Khon Kaen University) ;
  • Khampa, S. (Tropical Feed Resources Research and Development Center (TROFREC), Faculty of Agriculture, Khon Kaen University)
  • Received : 2005.06.05
  • Accepted : 2006.01.09
  • Published : 2007.01.01

Abstract

The object of this study was to determine the influence of supplementation of concentrate containing high levels of cassava chip on rumen ecology, microbial protein and digestibility of nutrients. Four, rumen fistulated crossbred beef steers with initial body weight of 400${\pm}$10 kg were randomly assigned according to a 4${\times}$4 Latin square design. The dietary treatments were concentrate cassava chip based offering at 0, 1, 2 and 3% BW with urea-treated rice straw fed ad libitum. It was found that ruminal pH was significantly decreased with increase of concentrate. Volatile fatty acids (VFA) concentration in the rumen was significantly different among treatments. In addition, a molar proportion of propionate was higher in supplemented groups at 2 and 3% BW (p<0.05), leading to significantly decreased acetate:propionate ratio. Furthermore, microbial N supply was significantly improved and was highest at 2% BW supplementation. The efficiency of rumen microbial-N synthesis based on organic matter (OM) truly digested in the rumen was highest in level of concentrate supplementation at 2% BW (80% of cassava chip in diets). Moreover, bacterial populations such as amylolytic bacteria was linearly increased, while cellulolytic bacteria was linearly decreased (p<0.01) when cattle received concentrate supplementation in all levels. The total protozoal counts were significantly increased, while fungal zoospores were dramatically decreased in cattle receiving increased levels of concentrate. In conclusion, cassava chip can be use as energy source at 80% in concentrate and supplementation of concentrate at 2% BW with urea-treated rice straw as roughage could improve rumen fermentation efficiency in beef cattle.

Keywords

References

  1. AOAC. 1985. Official Methods of Analysis. Association of Official Analysis Chemists,DC, USA.
  2. ARC. 1990. The Nutrient Requirements of Ruminant Livestock. Suppl.1. Commonwealth Agricultural Bureaux, Slough, Farnham Royal, UK
  3. Boniface, A. N., R. M. Murrah and J. P. Hogan. 1986. Optimum level of ammonia in the rumen liquid of cattle fed tropical pasture hay. Proc. Aust. Soc. Anim. Proc. 1:151-154.
  4. Chanjula, P., M. Wanapat, C. Wachirapakorn, S. Uriyapongson and P. Rowlinson. 2003. Ruminal degradability of tropical feeds and their potential use in ruminant diets. Asian-Aust. J. Anim. Sci. 16:211-216. https://doi.org/10.5713/ajas.2003.211
  5. Chanjula, P., M. Wanapat, C. Wachirapakorn and P. Rowlinson. 2004. Effect of synchronizing starch sources and protein (NPN) in the rumen on feed intake, rumen microbial fermentation, nutrient utilization and performance of lactating dairy cows. Asian-Aust. J. Anim. Sci. 17:1400-1410. https://doi.org/10.5713/ajas.2004.1400
  6. Chen, X. B. and M. J. Gomes. 1995. Estimation of Microbial Protein Supply to Sheep and Cattle Based on Urinary Excretion of Purine Derivatives-An Overview of the Technical Details. Occasional Publication 1992. International Feed Resources Unit, Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, UK.
  7. Chen, X. B., D. J. Kyle and E. R. Orskov. 1993. Measurement of allantoin in urine and plasma by high-performance liquid chromatography with pre-column derivatization. J. Chromatogr. 617: 241-247. https://doi.org/10.1016/0378-4347(93)80494-O
  8. Cheng, K. J., C. S. Stewart, D. Dinsdale and J. W. Costerton. 1984. Electron microscopy of bacteria involved in the digestion of plant cell walls. Anim. Feed Sci. Technol. 10:93-120. https://doi.org/10.1016/0377-8401(84)90002-6
  9. Crocker, C. L. 1967. Rapid determination of urea nitrogen in serum or plasma without deproteinzation. Am. J. Med. Techn. 33:361-365.
  10. Erdman, R. A. 1988. Dietary buffering requirements of the lactating dairy cows. A review. J. Dairy Sci. 71:3246-3266 https://doi.org/10.3168/jds.S0022-0302(88)79930-0
  11. France, J. and R. C. Siddons. 1993. Volatile fatty acid production. In: Quantilitive Aspects Ruminant Digestion and Metabolisim. (Ed. J. M. Forbes and J. France). pp. 107-122. CAB. International, Willingford, UK.
  12. Galyean, M. 1989. Laboratory Procedure in Animal Nutrition Research. Department of Animal and Life Science. New Mexico State University, USA.
  13. Goering, H. K. and Van Soest. 1970. Forage Fiber Analysis (apparatus, reagent, procedures and some application). Washington, DC: Agric. Handbook No. 379, ARS.
  14. Hong, N. T. T., M. Wanapat, C. Wachirapakorn, P. Pakdee and P. Rowlinson. 2003. Effects of timing of initial cutting and subsequent cutting on yields and chemical compositions of cassava hay and its supplementation on lactating dairy cows. Asian-Aust. J. Anim. Sci. 16:1763-1769. https://doi.org/10.5713/ajas.2003.1763
  15. Hoover, W. H. 1986. Chemical factors involved in ruminal fiber digestion. J. Dairy Sci. 69:2755-2766. https://doi.org/10.3168/jds.S0022-0302(86)80724-X
  16. Hoover, W. H. and S. R. Stokes. 1991. Balancing carbohydrate and proteins for optimum rumen microbial yield. J. Dairy Sci. 74:3640-3644.
  17. Hungate, R. E. 1969. A roll tube method for cultivation of stric anaerobes. In: Methods in Microbiology. (Ed. J. R. Norris and D. W. Ribbons). pp. 313-122. Academic. New York.
  18. IAEA. 1997. Estimation of the rumen microbial protein production from purine derivatives in rumen. Animal production and healt section. Vienna, Austria.
  19. Jouaney, J. P. and K. Ushida. 1999. The role of protozoa in feed digestion. Asian-Aust. J. Anim. Sci. 12:113-126. https://doi.org/10.5713/ajas.1999.113
  20. Khampa, S., M. Wanapat, C. Wachirapakorn, N. Nontaso and M. Wattiaux. 2006a. Effect of levels of sodium dl-malate supplementation on ruminal fermentation efficiency in concentrates containing high levels of cassava chip in dairy steers. Asian-Aust. J. Anim. Sci. 19:368-375. https://doi.org/10.5713/ajas.2006.368
  21. Khampa, S., M. Wanapat, C. Wachirapakorn, N. Nontaso and M. Wattiaux. 2006b. Effects of Urea Level and Sodium DLmalate in Concentrate Containing High Cassava Chip on Ruminal Fermentation Efficiency, Microbial Protein Synthesis in Lactating Dairy Cows Raised under Tropical Condition. Aisan-Aust. J. Anim. Sci. 19:837-844. https://doi.org/10.5713/ajas.2006.837
  22. Khang, D. N., H. Wiktorsson and T. R. Preston. 2005. and Chemical Composition of Cassava Foliage and Tuber Yield as Influenced by Harvesting Height and Cutting Interval. Asian-Aust. J. Anim. Sci. 18:1029-1035. https://doi.org/10.5713/ajas.2005.1029
  23. Kiyothong, K. and M. Wanapat. 2004. Growth, hay yield and chemical composition of cassava and Stylo 184 grown under intercropping. Aisan-Aust. J. Anim. Sci. 17:799-807. https://doi.org/10.5713/ajas.2004.799
  24. Krebs, G. and R. A. Leng. 1984. The effect of supplementation with molasses/urea blocks on ruminal digestion. Proc. Aust. Soc. Anim. Prod. 15:704-711.
  25. Mehrez, A. Z. and E. R. Orskov. 1978. Protein degradation and optimum urea concentration in cereal based diets for sheep. Br. J. Nutr. 40:337-345. https://doi.org/10.1079/BJN19780130
  26. Melaku, S., K. J. Peters and A. Tegegne. 2004. Microbial nitrogen supply, nitrogen retention and rumen function in Menz sheep supplemented with dried leaves of multipurpose trees, their mixtures or wheat bran. Small Rum. Res. 52:25-36. https://doi.org/10.1016/S0921-4488(03)00200-1
  27. Mould, F. L. and E. R. Orskov. 1984. Manipulation of rumen fluid pH and its influence on cellulolysis in sacco, dry matter degradation and the rumen microflora of sheep offered either hay or concentrate. Anim. Feed Sci. Technol. 10:1-14. https://doi.org/10.1016/0377-8401(83)90002-0
  28. Owens, F. N. and R. Zinn. 1988. Protein metabolism of ruminant animals. In: The Ruminant Animal Digestive Physiology and Nutrition (Ed. D. C. Church). pp. 227-249. Waveland Press Inc., Prospect Hights, IL, USA.
  29. Perdok, H. B. and R. A. Leng. 1990. Effect of supplementation with protein meal on the growth of cattle given a basal diet of untreated or ammoniated rice straw. Asian-Aust. J. Anim. Sci. 3:269-279. https://doi.org/10.5713/ajas.1990.269
  30. Preston, R. L., D. D. Schnakanberg and W. H. Pander. 1965. Protein utilization in ruminants. I. Blood urea nitrogen as affected by protein intake. J. Nutr. 86:281-287. https://doi.org/10.1093/jn/86.3.281
  31. Promkot, C. and M. Wanapat. 2005. Effect of level of crude protein and use of cottonseed meal in diets containg cassava chips and rice straw for lactating dairy cows. Asian-Aust. J. Anim. Sci. 18:502-511. https://doi.org/10.5713/ajas.2005.502
  32. Robinson, P. H., S. Tamminga and A. M. Van Vuuren. 1986. Influence of declining level of feed intake and varying the proportion of starch in the concentrate on rumen fermentation in dairy cows. Livestock Prod. Sci. 15:173-189. https://doi.org/10.1016/0301-6226(86)90026-6
  33. Samuel, M., S. Sagathewan, J. Thomas and G. Mathen. 1997. An HPLC method for estimation of volatile fatty acids of ruminal fluid. Indian J. Anim. Sci. 69:805-807.
  34. SAS. 1998. User's Guide: Statistics, Version 7th Edition. SAS Inst. Inc., Cary, NC.
  35. Satter, L. D. and L. L. Slyter. 1974. Effect of ammonia concentration on rumen microbial protein production in vitro. Br. J. Nutri. 32:199-208. https://doi.org/10.1079/BJN19740073
  36. Sommart, K., D. S. Parker, M. Wanapat and P. Rowlinson. 2000. Fermentation characteristics and microbial protein synthesis in an in vitro system using cassava, rice straw and dried ruzi grass as substrates. Asian-Aust. J. Anim. Sci. 13:1084-1093. https://doi.org/10.5713/ajas.2000.1084
  37. Steel, R. G. D. and J. H. Torrie. 1980. Principles and Procedure of Statistics. McGraw Hill Book Co. New York.
  38. Sutton, J. D., S. V. Morant, J. A. Bines, D. J. Napper and D. I. Givens. 1993. Effect of altering the starch:fibre ratio in the concentrates on hay intake and milk production by Friesian cows. J. Agric. Sci. (Camb). 120:379-390. https://doi.org/10.1017/S0021859600076541
  39. Van Keulen, J. and B. A. Young. 1977. Evaluation of acid insoluble ash as a neutral marker in ruminant digestibility studies. J. Anim. Sci. 44:282-287. https://doi.org/10.2527/jas1977.442282x
  40. Wanapat, M. 1990. Nutritional Aspects of Ruminant Production in Southeast Asia with Special Reference to Thailand. Funny Press, Ltd., Bangkok, Thailand.
  41. Wanapat, M. 2000. Rumen manipulation to increase the efficient use of local feed resources and productivity of ruminants in the tropics. Asian-Aust. J. Anim. Sci. 13 (Suppl.):59-67.
  42. Wanapat, M. 2003. Manipulation of cassava cultivation and utilization to improve protein to energy biomass for livestock feeding in the tropics. Asian-Aust. J. Anim. Sci. 16:463-472. https://doi.org/10.5713/ajas.2003.463
  43. Wanapat, M. and O. Pimpa. 1999. Effect of ruminal NH3-N levels on ruminal fermentaion, purine derivatives,digestibility and rice straw intake in swamp buffaloes. Asian-Aust. J. Anim. Sci. 12:904-907. https://doi.org/10.5713/ajas.1999.904

Cited by

  1. Enriching nutritive value of cassava root by yeast fermentation vol.66, pp.5, 2009, https://doi.org/10.1590/S0103-90162009000500007
  2. Effect of carbohydrate sources and cotton seed meal in the concentrate: II. Feed intake, nutrient digestibility, rumen fermentation and microbial protein synthesis in beef cattle vol.44, pp.1, 2012, https://doi.org/10.1007/s11250-011-0014-z
  3. Effect of carbohydrate source and cottonseed meal level in the concentrate: IV. Feed intake, rumen fermentation and milk production in milking cows vol.45, pp.2, 2013, https://doi.org/10.1007/s11250-012-0238-6
  4. Practice on improving fattening local cattle production in Vietnam by increasing crude protein level in concentrate and concentrate level vol.45, pp.7, 2013, https://doi.org/10.1007/s11250-013-0407-2
  5. Influence of varying levels of supplemental cassava root meal without or with groundnut cake on performance of growing Laisind cattle vol.46, pp.6, 2014, https://doi.org/10.1007/s11250-014-0586-5
  6. Effect of concentrate supplementation on nutrient digestibility and growth of Brahman crossbred cattle fed a basal diet of grass and rice straw vol.57, pp.1, 2015, https://doi.org/10.1186/s40781-015-0068-y
  7. Influence of Aspergillus niger or Saccharomyces cerevisiae-Fermented Napier Grass (Pennisetum purpureum) Mixed with Fresh Cassava Root on Blood Parameters and Nutrient Digestibility in Growing Beef Cattle vol.16, pp.10, 2017, https://doi.org/10.3923/pjn.2017.776.781
  8. The Effect of Coleus amboinicus L. Supplementation on In vitro Digestibility vol.18, pp.3, 2019, https://doi.org/10.3923/pjn.2019.241.246
  9. Influence of Sulfur on Fresh Cassava Foliage and Cassava Hay Incubated in Rumen Fluid of Beef Cattle vol.20, pp.9, 2007, https://doi.org/10.5713/ajas.2007.1424
  10. Effects of Replacing Ground Corn with Cassava Chip in Concentrate on Feed Intake, Nutrient Utilization, Rumen Fermentation Characteristics and Microbial Populations in Goats vol.20, pp.10, 2007, https://doi.org/10.5713/ajas.2007.1557
  11. Effect of Roughage Sources on Cellulolytic Bacteria and Rumen Ecology of Beef Cattle vol.20, pp.11, 2007, https://doi.org/10.5713/ajas.2007.1705
  12. Effects of Level and Degradability of Dietary Protein on Ruminal Fermentation and Concentrations of Soluble Non-ammonia Nitrogen in Ruminal and Omasal Digesta of Hanwoo Steers vol.21, pp.3, 2007, https://doi.org/10.5713/ajas.2008.70342
  13. Amount of Cassava Powder Fed as a Supplement Affects Feed Intake and Live Weight Gain in Laisind Cattle in Vietnam vol.21, pp.8, 2008, https://doi.org/10.5713/ajas.2008.70479
  14. Effects of Amount of Concentrate Supplement on Forage Intake, Diet Digestibility and Live Weight Gain in Yellow Cattle in Vietnam vol.21, pp.12, 2007, https://doi.org/10.5713/ajas.2008.80082
  15. Effects of Sago Palm Pith as Replacement for Corn Grain on Intake, Rumen Fermentation Characteristics and Microbial N Supply of Cattle Fed Paspalum plicatulum Hay vol.22, pp.3, 2009, https://doi.org/10.5713/ajas.2009.80356
  16. Influence of urea calcium mixture supplementation on ruminal fermentation characteristics of beef cattle fed on concentrates containing high levels of cassava chips and rice straw vol.163, pp.1, 2011, https://doi.org/10.1016/j.anifeedsci.2010.10.003
  17. Effects of protein levels and energy sources in total mixed ration on feedlot performance and carcass quality of Kamphaeng Saen steers vol.51, pp.1, 2007, https://doi.org/10.1016/j.anres.2017.02.003
  18. Effects of Sulfur Levels in Fermented Total Mixed Ration Containing Fresh Cassava Root on Feed Utilization, Rumen Characteristics, Microbial Protein Synthesis, and Blood Metabolites in Thai Native Bee vol.9, pp.5, 2007, https://doi.org/10.3390/ani9050261
  19. Effect of Using Different Levels of Cassava Meal on Nutrient Intake, Fiber Digestibility and Body Condition Score in Crossbreed Limousin Bulls vol.478, pp.None, 2007, https://doi.org/10.1088/1755-1315/478/1/012065
  20. Screening of Cyanide-Utilizing Bacteria from Rumen and In Vitro Evaluation of Fresh Cassava Root Utilization with Pellet Containing High Sulfur Diet vol.8, pp.1, 2007, https://doi.org/10.3390/vetsci8010010
  21. Effect of feeding a pellet diet containing high sulphur with fresh cassava root supplementation on feed use efficiency, ruminal characteristics and blood metabolites in Thai native beef cattle vol.105, pp.4, 2007, https://doi.org/10.1111/jpn.13514
  22. Sulfur, fresh cassava root and urea independently enhanced gas production, ruminal characteristics and in vitro degradability vol.17, pp.1, 2007, https://doi.org/10.1186/s12917-021-02999-3