Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Ameliorating Effect of Gardenia jasminoides Extract on Amyloid Beta Peptide-induced Neuronal Cell Deficit

  • Choi, Soo Jung (Department of Food Technology, Korea University) ;
  • Kim, Mi-Jeong (Department of Food Technology, Korea University) ;
  • Heo, Ho Jin (Department of Food Science & Technology and Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Hong, Bumshik (Department of Food and Biotechnology, Korea University) ;
  • Cho, Hong Yon (Department of Food and Biotechnology, Korea University) ;
  • Kim, Young Jun (Department of Food and Biotechnology, Korea University) ;
  • Kim, Hye Kyung (Department of Food and Biotechnology, Hanseo University) ;
  • Lim, Seung-Taik (Department of Food Technology, Korea University) ;
  • Jun, Woo Jin (Department of Food and Nutrition, Chonnam University) ;
  • Kim, Eun-Ki (Department of Biological Engineering, Inha University) ;
  • Shin, Dong-Hoon (Department of Food and Biotechnology, Korea University)
  • Received : 2007.03.03
  • Accepted : 2007.04.27
  • Published : 2007.08.31
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Abstract

The brains of Alzheimer's disease (AD) patients are characterized by large deposits of amyloid beta peptide ($A{\beta}$). $A{\beta}$ is known to increase free radical production in nerve cells, leading to cell death that is characterized by lipid peroxidation, free radical formation, protein oxidation, and DNA/RNA oxidation. In this study, we selected an extract of Gardenia jasminoides by screening, and investigated its ameliorating effects on $A{\beta}$-induced oxidative stress using PC12 cells. The effects of the extract were evaluated using the 2',7'-dichlorofluorescein diacetate (DCF-DA) assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. To find the active component, the ethanol extract was partitioned with hexane, chloroform, and ethyl acetate, respectively, and the active component was purified by silica-gel column chromatography and HPLC. The results suggested that Gardenia jasminoides extract can reduce the cytotoxicity of $A{\beta}$ in PC 12 cells, possibly by reducing oxidative stress.

Keywords

Acknowledgement

Supported by : Rural Development Administration

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