BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
권호 표지
DOI QR코드

DOI QR Code

Helicoverpa armigera Nucleopolyhedrovirus ORF80 Encodes a Late, Nonstructural Protein

  • Wang, Dun (Institute of Insect Sciences, Zhejiang University) ;
  • Zhang, Chuan-Xi (Institute of Insect Sciences, Zhejiang University)
  • 발행 : 2007.01.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The Helicoverpa armigera nucleopolyhedrovirus (HearNPV) ORF80 (ha80) has 765 bp encoding a protein with approximately 254 amino acids and a predicted molecular weight of 30.8 kDa. Homologues of ha80 are found in most baculovirus sequences, including those from lepidopteran NPVs, lepidopteran granuloviruses (GVs), hymenopteran baculoviruses, and one dipteran baculovirus, yet their functions remain unclear. In this study we characterized ha80, and showed that it was transcribed late in infected host cells (HzAM1). The product of ha80 was a 31 kDa protein that was not a structural protein of budded virus (BV) or occlusion-derived virus (ODV) particles. Ha80 was first detected in the cytoplasm of infected HzAM1 cells at 12 h p.i., and was observed in the nucleus at later stages of infection, suggesting that it may be involved in transporting viral proteins into the host cell nucleus or play its roles in the nucleus.

키워드

참고문헌

  1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D. J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402. https://doi.org/10.1093/nar/25.17.3389
  2. An, S. H., Shang, J. Y., Liu, Y. H., Kumar, V. S., Guo, Z. J., Wang., D., Yang, Z. N. and Zhang, C. X. (2006) Characterization of a unique gene ORF135 from Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus. Virus Genes 32, 21-26. https://doi.org/10.1007/s11262-005-5841-4
  3. An, S. H, Wang, D., Yang, Z. N., Guo, Z. J., Xu, H. J., Sun J. X and Zhang C. X. (2005) Characterization of a late expression gene, Open reading frame 128 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus. Arch. Virol. 150, 2453-2466. https://doi.org/10.1007/s00705-005-0605-y
  4. An, S. H., Xing L. P., Kumar, V. S. and Zhang C. X. (2005) Analysis of a late gene, orf101 from Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus. Insect Science 12, 335-340. https://doi.org/10.1111/j.1005-295X.2005.00041.x
  5. Beniya, H., Braunagel, S. C. and Summers, M. D. (1998) Autographa californica nuclear polyhedrosis virus: subcellular localization and protein trafficking of BV/ODV-E26 to intranuclear membranes and viral envelopes. Virology 240, 64-75. https://doi.org/10.1006/viro.1997.8903
  6. Braunagel, S. C., Burks, J. K., Rosas-Acosta, G., Harrison, R. L., Ma, H. and Summers, M. D. (1999) Mutations within the Autographa californica nucleopolyhedrovirus FP25K gene decrease the accumulation of ODV-E66 and alter its intranuclear transport. J. Virol. 73, 8559-8570.
  7. Chen, X., Ijkel, W. F. J., Dominy, C., Zanotto, P. M. A., Hashimoto, Y., Fakto, O., Hayakawa, T., Wang, C. H., Prekumar, A., Mathavan, S., Krell, P. J., Hu, Z. and Vlak, J. M. (1999) Identification, sequence analysis and phylogeny of the lef-2 gene of single-nucleocapsid baculovirus. Virus Res. 65, 21-32. https://doi.org/10.1016/S0168-1702(99)00097-0
  8. Chen, X., IJkel, W. F., Tarchini, R., Sun, X., Sandbrink, H., Wang, H., Peters, S., Zuidema, D., Lankhorst, R. K., Vlak, J. M. and Hu, Z. (2001) The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome. J. Gen. Virol. 82, 241-257. https://doi.org/10.1099/0022-1317-82-1-241
  9. Dong, C., Li, D., Long, G., Deng, F., Wang, H. and Hu, Z. (2005) Identification of functional domains of HearNPV P10 for filament formation. Virology 338, 112-120. https://doi.org/10.1016/j.virol.2005.05.003
  10. Fang, M. G., Wang, H. Z., Wang, H. L., Yuan, L., Chen, X., Vlak, J. M. and Hu, Z. H. (2003) Open reading frame 94 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel conserved occlusion-derived virion protein, ODV-EC43. J. Gen. Virol. 84, 3021-3027. https://doi.org/10.1099/vir.0.19291-0
  11. Guarino, L. A., Smith, G. and Dong, W. (1995) Ubiquitin is attached to membranes of baculovirus particles by a novel type of phospholipid anchor. Cell 80, 301-309. https://doi.org/10.1016/0092-8674(95)90413-1
  12. Guo, Z. J., An, S. H., Wang, D., Liu, Y. H., Kumar, S. and Zhang, C. X. (2005) Characterization of Ha29, a specific gene for Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus. J. Biochem. Mol. Biol. 38, 354-359. https://doi.org/10.5483/BMBRep.2005.38.3.354
  13. Hong, T., Summers, M. D. and Braunagel, S. C. (1997) Nterminal sequences from Autographa californica nuclear polyhedrosis virus envelope protein ODV-E66 and ODV-E25 are sufficient to direct reporter proteins to the nuclear envelop, intranuclear microvesicles and the envelope of occlusion - derived virus. Proc. Natl. Acad. Sci. USA 94, 4050-4055. https://doi.org/10.1073/pnas.94.8.4050
  14. IJkel, W. F. J., Lebbink, R. J., Op den Brouw, M. L., Goldbach, R. W., Vlak, J. M. and Zuidema, D. (2001) Identification of a novel occlusion derived virus-specific protein in Spodoptera exigua multicapsid nucleopolyhedrosis virus. Virology 284, 170-181. https://doi.org/10.1006/viro.2001.0906
  15. Long, G., Westenberg, M., Wang, H., Vlak, J. M. and Hu, Z. (2006) Function, oligomerization and N-linked glycosylation of the Helicoverpa armigera single nucleopolyhedrovirus envelope fusion protein. J. Gen. Virol. 87, 839-846. https://doi.org/10.1099/vir.0.81592-0
  16. McIntosh, A. H. and Ignoffo, C. M. (1983) Characterization of five cell lines established from species of Heliothis. Appl. Entomol. Zool. 18, 262-269. https://doi.org/10.1303/aez.18.262
  17. Nie, Y. C., Wang, Q., Liang, C. Y., Fang, M. G., Yu, Z. H. and Chen, X. W. (2006) Characterization of ORF2 and its encoded protein of the Helicoverpa armigera nucleopolyhedrovirus. Virus Res. 116, 129-135. https://doi.org/10.1016/j.virusres.2005.09.007
  18. O'Reilly, D. R., Miller, L. K. and Luckow, V. A. (1992) Baculovirus Expression Vectors: A Laboratory Manual, W. H. Freeman & Co., New York.
  19. Rohrmann, G. F. (1999) Nuclear polyhedrosis viruses; in Encyclopedia of virology, Webster, R. G. and Granoff, A. (eds.), pp. 146-152, Academic Press, London, UK.
  20. Spector, D. L., Goldman, R. D. and Leinwand, L. A. (1998) Cells: A Laboratory Manual, vol. 3: Subcellular localization of genes and their products, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA.
  21. Sun, X. L., Zhang, G. Y., Zhang, Z. X., Hu, Z. H., Vlak, J. M. and Arif, B. M. (1998) In vivo cloning of Helicoverpa armigera single nucleocapsid nuclear polyhedrosis virus genotypes. Virol Sin. 13, 83-88.
  22. Wang, D., An, S. H., Guo, Z. J., Xu, H. J. and Zhang, C. X. (2005) Characterization of Helicoverpa armigera nucleopolyhedrovirus orf33 that encodes a novel budded virion derived protein, BV-e31. Arch. Virol. 150, 1505-1515. https://doi.org/10.1007/s00705-005-0534-9
  23. Wang, D. and Zhang, C. X. (2006) HearSNPV orf83 encodes a late, nonstructural protein with an active chitin binding domain. Virus Res. 117, 237-243. https://doi.org/10.1016/j.virusres.2005.10.019
  24. Winstanley, D. and O'Reilly, D. R. (1999) Granuloviruses; in Encyclopedia of virology, Webster, R. G. and Granoff, A. (eds.), pp. 140-146, Academic Press, London, UK.
  25. Wu, D., Deng, F., Sun, X., Wang, H., Yuan, L., Vlak, J. M. and Hu, Z. (2005) Functional analysis of FP25K of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus. J. Gen. Virol. 86, 2439-2444. https://doi.org/10.1099/vir.0.81110-0
  26. Xu, H. J., Liu, Y. H., Yang, Z. N. and Zhang, C. X. (2006) Characterization of ORF39 from Helicoverpa armigera Single nucleocapsid Nucleopolyhedrovirus, the Gene Containing RNA Recognition Motif. J. Biochem. Mol. Biol. 39, 263-269. https://doi.org/10.5483/BMBRep.2006.39.3.263
  27. Zhang, C. X., Ma, X. C. and Guo, Z. J. (2005) Comparison of the complete genome sequences between C1 and G4 isolates of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus. Virology 333, 190-199. https://doi.org/10.1016/j.virol.2004.12.028
  28. Zhang, G., Zhang, Y., Ge, L. and Shan, Z. (1981) The production and application of the nuclear polyhedrosis virus of Heliothis armigera (Hubner) in biological control. Acta Phytophyl. Sini. 8, 235-240.