Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of a Lactobacillus acidophilus Strain Isolated from Korean Infant Feces and Cloning of Surface Layer Protein Gene slp and Its Expression in Escherichia coli

유아 분변에서 분리한 Lactobacillus acidophilus의 특성 및 표면 단백질 유전자 클로닝과 대장균 내에서의 발현

  • Park, Myeong-Soo (Department of Nutrition and Hotel Culinary Art, Anyang Technical College) ;
  • Ji, Geun-Eog (Department of Food and Nutrition, Seoul National University) ;
  • You, Kwan-Hee (Department of Biological Science, Sangji University) ;
  • Lee, Si-Kyung (Department of Applied Biology and Chemistry, Konkuk University) ;
  • Jeong, Won-Seok (Department of Biological Sciences, Konkuk University) ;
  • Kim, Jin-Hyung (Department of Biological Sciences, Konkuk University) ;
  • Jo, Myoung-Hwan (Department of Biological Sciences, Konkuk University) ;
  • Kim, Soo-Young (Department of Biological Sciences, Konkuk University)
  • 박명수 (안양과학대학 호텔조리영양학부) ;
  • 지근억 (서울대학교 생활과학대학 식품영양학과) ;
  • 유관희 (상지대학교 이공과대학 생명과학과) ;
  • 이시경 (건국대학교 생명환경과학대학) ;
  • 정원석 (건국대학교 이과대학 생명과학과) ;
  • 김진형 (건국대학교 이과대학 생명과학과) ;
  • 조명환 (건국대학교 이과대학 생명과학과) ;
  • 김수영 (건국대학교 이과대학 생명과학과)
  • Published : 2007.12.28
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Abstract

A Lactobacillus sp. has been isolated from infant feces and characterized according to its physiological properties and identified as Lactobacillus acidophilus KLA1012. A gene coding surface layer protein (SLP) has been cloned and the sequence has been determined. The nucleotide sequence of slpA was 1,338 bp in size and was identical to that of L. acidophilus ATCC 4356 (100%). Amino acid sequence of SLP-A was deduced from the nucleotide sequence and it had signal sequence at N-terminal, consisting of positively charged amino acid mainly lysine. slpA was cloned and heterologously expressed in E. coli M15 and the 45.2 kDa surface-layer protein band was examined by SDS-PAGE and confirmed by Western blotting using polyclonal antibody against L. acidophilus KLA 1012 SLP-A protein.

Keywords

References

  1. Arai, S., T. Osawa, H. Ohigashi, M. Yoshikawa, S. Kaminogawa, M. Watanabe, T. Ogawa, K. Okubo, S. Watanabe, H. Nishino, K.. Shinohara, T. Esashi, and T. Hirahara. 2001. A mainstay of functional food science in Japan-History, present status, and future outlook. Biosci. Biotechnol. Biochem. 65: 1-13 https://doi.org/10.1271/bbb.65.1
  2. Beachey, E. H. 1981. Bacterial adherence: adhesin receptor interactions mediating the attachment of bacteria to mucosal surfaces. J. Infect. Dis. 143: 325-345 https://doi.org/10.1093/infdis/143.3.325
  3. Beveridge, T. J. and L. L. Graham. 1991. Surface layers of bacteria. Microbiol. Rev. 55: 684-705
  4. Bhowmik, T., M. C. Johnson, and B. Ray. 1985. Isolation and partial characterization of the surface protein of Lactobacillus acidophilus strains. Int. J. Food Microbiol. 2: 311-321 https://doi.org/10.1016/0168-1605(85)90044-3
  5. Boot, H. J., C. P. Kolen, J. M. van Noort, and P. H. Pouwels. 1993. S-Layer Protein of Lactobacillus acidophilus ATCC 4356 : Purification, expression in Escherichia coli, and nucleotide sequence of the corresponding gene, J. Bacteriol. 175: 6089-6096 https://doi.org/10.1128/jb.175.19.6089-6096.1993
  6. Boot, H. J., C. P. Kolen, and P. H. Pouwels. 1995. Identification, cloning, and nucleotide sequence of a silent S-Layer protein gene of Lactobacillus acidophilus ATCC 4356 which has extensive similarity with the S-Layer Protein gene of this species, J. Bacteriol. 177: 7222-7230 https://doi.org/10.1128/jb.177.24.7222-7230.1995
  7. Callegari, M. L., B. Riboli, J. W. Sanders, P. S. Cocconcelli, J. Kok, G. Venema, and L. Morelli. 1998. The S-layer gene of Lactobacillus helveticus CNRZ 892 : cloning, sequence and heterologous expression. Microbiol. 144: 719-726 https://doi.org/10.1099/00221287-144-3-719
  8. Cowan, S. T. 1974. Manual for the Identification of Medical Bacteria, pp. 147-161. 2nd ed. Cambridge University Press, Cambridge, UK
  9. Doig, P., L. Emdy, and I. J. Trust. 1992. Binding of laminin and fibronectin by the trypsin-resistant major structural domain of the crystalline virulence surface array protein of Aeromonas salmonicida. J. Biol. Chem. 267: 43-49
  10. Egbert, S., and H. P. Peter. 2002. One repeat of the cell wall binding domain is sufficient for anchoring the Lactobacillus acidophilus surface layer protein. J. Bacteriol. 184: 4617-4616 https://doi.org/10.1128/JB.184.16.4617-4619.2002
  11. Fernandes, C. F., K. M. Shahani, and M. A. Amer. 1987. Therapeutic role of dietary Lactobacilli and lactobacillic fermented dairy products. FEMS Microbiol. Rev. 46: 343-356 https://doi.org/10.1111/j.1574-6968.1987.tb02471.x
  12. Fischetti, V. A., D. Medaglini, M. Oggioni, and G. Pozzi. 1993. Expression of foreign proteins on Gram-positive commensal bacteria for mucosal vaccine delivery. Curr. Opin. Biotechnol. 4: 603-610 https://doi.org/10.1016/0958-1669(93)90084-A
  13. Georgious, G., H. L. Poetschke, C. Stathopoulos, and J. A. Francisco. 1993. Practical application for engineering Gramnegative bacteria cell surface. Tibitech. 11: 6-10 https://doi.org/10.1016/0167-7799(93)90068-K
  14. Ishiguro, E. E., W. W. Kay, T. Ainsworth, J. B. Chamberlain, R. A. Austen, J. T. Buckley, and T. J. Trust. 1981. Loss of virulence during culture of Aeromonas salmonicida at high temperature. J. Bacteriol. 148: 333-340
  15. Isolauri, E., J. Joensuu, H. Suomalainen, M. Luomala, and T. Vesikari. 1995. Improved immunogenicity of oral Dx RRV reassortant rotavirus vaccine by Lactabacillus casei GG. Vaccine 13: 310-312 https://doi.org/10.1016/0264-410X(95)93319-5
  16. Kitazawa, H., K. Matsumura, T. Itoh, and T. Yamaguch. 1992. Interferon induction in murine peritoneal macrophage by stimulation with Lactobacillus acidophilus. Microbiol. Immunol. 36: 311-315 https://doi.org/10.1111/j.1348-0421.1992.tb01668.x
  17. Klaenhammer, T. R. 1993. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12: 39-85
  18. Masuda, K. and T. Kawata. 1979. Ultrastructure and partial characterization of a regular array in the cell wall of Lactobacillus brevis. Microbiol. Immunol. 23: 941-953 https://doi.org/10.1111/j.1348-0421.1979.tb00525.x
  19. Masuda, K. and T. Kawata. 1980. Reassembly of the regularly arranged subunits in the cell wall of Lactobacillus brevis and their reattachment to cell wall. Microbiol. Immunol. 24: 299-308 https://doi.org/10.1111/j.1348-0421.1980.tb02833.x
  20. Munn, C. B., E. E. Ishiguro, W. W. Kay, and T. J. Trust. 1982. Role of surface components in serum resistance of virulent Aeromonas salmonicida. Infect. Immun. 36: 1069-1075
  21. Orrhage, K., E. Sillerstrm, J. A. Gustafsson, C. E. Nord, and J. Rafter. 1994. Binding of mutagenic heterocyclic amines by intestinal and lactic acid bacteria. Mutat. Res. 311: 239-248 https://doi.org/10.1016/0027-5107(94)90182-1
  22. O'Sullivan, M. G., G. Thornton, G. C. O'Sullivan, and J. K. Collins. 1992. Probiotic bacteria: Myth or reality. Trends Food Sci. Technol. 3: 309-314 https://doi.org/10.1016/S0924-2244(10)80018-4
  23. Pum, D., P. Messner, and U. B. Sleytr. 1991. Role of the S layer in morphogenesis and cell division of the archaebacteriumMethanocopusculum sinense. J. Bacteriol. 173: 6865-6873 https://doi.org/10.1128/jb.173.21.6865-6873.1991
  24. Sra, M. and U. B. Sleytr. 1987. Molecular sieving through S layers of Bacillus stearothermophilus strains. J. Bacteriol. 169: 4092-4098 https://doi.org/10.1128/jb.169.9.4092-4098.1987
  25. Sra, M., and U. B. Sleytr. 2000. S-layer protein. J. Bacteriol. 182: 859-868 https://doi.org/10.1128/JB.182.4.859-868.2000
  26. Simon, G. L. and S. L. Gorbach. 1986. The human intestinal rnicroflora. Dig. Dis. Sci. 31: 1475-1625
  27. Steer, T., H. Carpenter, K. Tuohy, and G. R. Gibson. 2000. Perspectives on the role of the human gut rnicrobiota and its modulation by pro- and prebiotics. Nutr. Res. Rev. 13: 229-254 https://doi.org/10.1079/095442200108729089
  28. Vidgren, G., I. Palva, R. Pakkanen, K. Lounatmaa, and A. Palva. 1992. S-layer protein gene of Lactobacillus brevis: Cloning by polymerase chain reaction and determination of the nucleotide sequence. J. Bacteriol. 174: 7419-7427 https://doi.org/10.1128/jb.174.22.7419-7427.1992