Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Continuous Production of Lactosucrose by Immobilized Sterigmatomyces elviae Mutant

  • Lee, Jong-Ho (Department of Chemical and Biological Engineering, Korea University) ;
  • Lim, Jung-Soo (Digital Appliances R&D Team, Samsung Electronics Co. Ltd.) ;
  • Park, Chul-Hwan (Department of Chemical Engineering, Kwangwon University) ;
  • Kang, Seong-Woo (Department of Chemical and Biological Engineering, Korea University) ;
  • Shin, Hyun-Yong (Department of Chemical and Biological Engineering, Korea University) ;
  • Park, Seung-Won (Food Ingredient Division, CJ Food R&D, CJ Corp.) ;
  • Kim, Seung-Wook (Department of Chemical and Biological Engineering, Korea University)
  • Published : 2007.09.30
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Abstract

In this study, in order to develop a continuous production process of lactosucrose in a packed-bed reactor, Sterigmatomyces elviae ATCC 18894 was selected and mutated. The mutant strain of S. elviae showed 54.3% higher lactosucrose production than the wild type. Reaction conditions such as temperature, pH, substrate concentration and flow rate were also optimized. Under optimized reaction conditions ($50^{\circ}C$, pH 6.0, 25% sucrose and 25% lactose as substrate, flow rate 1.2 ml/min), the maximum concentration of lactosucrose (192 g/l) was obtained. In a packed-bed reactor, continuous production of lactosucrose was performed using S. elviae mutant immobilized in calcium alginate, and about 180 g/l of lactosucrose production was achieved for 48 days.

Keywords

References

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