Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Zinc Metal Solubilization by Gluconacetobacter diazotrophicus and Induction of Pleomorphic Cells

  • Saravanan, Venkatakrishnan Sivaraj (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Osborne, Jabez (School of Bio-Technology, Chemical and Bio-Medical Engineering, Vellore Institute of Technology (VIT) University) ;
  • Madhaiyan, Munusamy (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Mathew, Lazar (School of Bio-Technology, Chemical and Bio-Medical Engineering, Vellore Institute of Technology (VIT) University) ;
  • Chung, Jong-Bae (Division of Life and Environmental Sciences, Daegu University) ;
  • Ahn, Ki-Sup (Department of Health and Environmental Science, Baekseok College of Cultural Studies) ;
  • Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
  • Published : 2007.09.30
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Abstract

Gluconacetobacter diazotrophicus strain PA15 exhibited a minimum inhibitory concentration value of 11 mM in an LGI medium amended with $ZnCl_2$. When an LGI medium was amended with Zn metal, solubilization halos were observed in a plate assay, and further solubilization was confirmed in a broth assay. The maximum solubilization was recorded after 120 h with a 0.1% Zn metal amendment. During solubilization, the culture growth and pH of the broth were indirectly correlated. Using a Fourier Transform Infrared Spectroscopy analysis, one of the agents solubilizing the Zn metal was identified as gluconic acid. When the Zn-amended broth was observed under a bright field microscope, long involution cells were observed, and further analysis with Atomic Force Microscopy revealed highly deformed, pleomorphic, aggregate-like cells.

Keywords

References

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