Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Functional Assembly of Recombinant Human Ferritin Subunits in Pichia pastoris

  • Lee, Jung-Lim (Department of Food Science, Massachusetts Agricultural Experiment Station, University of Massachusetts) ;
  • Park, Cheon-Seok (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Hae-Yeong (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
  • Published : 2007.10.30
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Abstract

Ferritin is an iron storage protein found in most living organisms as a natural assembled macromolecule. For studying the functional ability of the ferritin assembly, human H- and L-ferritins were expressed and purified from Pichia pastoris strain GS115. The recombinant H- and L-ferritins showed a globular form with transmission electron microscopy. The rate of iron uptake for H-ferritin was significantly faster than that for the L-ferritin in vitro. By gel permeation chromatography analysis, recombinant ferritins were confirmed as multimeric subunits with high molecular weight and it was indicated that assembled subunits were able to store iron in vivo.

Keywords

References

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