Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning and Expression of a Parathion Hydrolase Gene from a Soil Bacterium, Burkholderia sp. JBA3

  • Kim, Tae-Sung (Environmental Biosafety Division, Nature and Ecology Research Department, National Institute of Environmental Research) ;
  • Ahn, Jae-Hyung (Department of Agricultural Biotechnology, Seoul National University) ;
  • Choi, Min-Kyeong (Department of Agricultural Biotechnology, Seoul National University) ;
  • Weon, Hang-Yeon (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Mi-Sun (Department of Biology, Sunchon National University) ;
  • Seong, Chi-Nam (Department of Biology, Sunchon National University) ;
  • Song, Hong-Gyu (Division of Biological Sciences, Kangwon National University) ;
  • Ka, Jong-Ok (Department of Agricultural Biotechnology, Seoul National University)
  • Published : 2007.11.30
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Abstract

A bacterium, Burkholderia sp. JBA3, which can mineralize the pesticide parathion, was isolated from an agricultural soil. The strain JBA3 hydrolyzed parathion to p-nitrophenol, which was further utilized as the carbon and energy sources. The parathion hydrolase was encoded by a gene on a plasmid that strain JBA3 harbored, and it was cloned into pUC19 as a 3.7-kbp Sau3AI fragment. The ORF2 (ophB) in the cloned fragment encoded the parathion hydrolase composed of 526 amino acids, which was expressed in E. coli DH10B. The ophB gene showed no significant sequence similarity to most of other reported parathion hydrolase genes.

Keywords

References

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