Journal of Microbiology and Biotechnology

  • 제17권12호
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  • Pages.2081-2084
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  • 2007
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Expression of ${\alpha}$-Galactosidase Gene from Leuconostoc mesenteroides SY1 in Leuconostoc citreum

  • Park, Jae-Yong (Institute of Argriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Seon-Ju (Institute of Argriculture and Life Science, Gyeongsang National University) ;
  • Lee, Ae-Ran (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Park, Ji-Yeong (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Jeong, Woo-Ju (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Kim, Jeong-Hwan (Institute of Argriculture and Life Science, Gyeongsang National University)
  • 발행 : 2007.12.31
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초록

A 2.5 kb aga gene encoding ${\alpha}$-galactosidase (${\alpha}$-Gal) from Leuconostoc mesenteroides SY1 was cloned into pSJE, an E. coli-Leuconostoc shuttle vector. The recombinant plasmid, pSJEaga, was introduced into Leuconostoc citreum KCTC3526 (ATCC49370) by electroporation. Transcription level of aga was the highest in cells grown on raffinose (1%, w/v) followed by cells grown on galactose, melibiose, fructose, glucose, and sucrose. Western blot using antibodies against ${\alpha}$-Gal showed similar results to slot-blot results and enzyme activity measurements. All the results indicated that the aga was successfully expressed in L. citreum and its transcription was under the carbon catabolite repression (CCR).

키워드

참고문헌

  1. Choi, I. K., S. H. Jung, B. J. Kim, S. Y. Park, J. Kim, and H. U. Han. 2003. Novel Leuconostoc citreum starter culture system for the fermentation of kimchi, a fermented cabbage product. Antonie van Leeuwenhoek 84: 247-253 https://doi.org/10.1023/A:1026050410724
  2. Cho, J., D. Lee, C. Yang, J. Jeon, J. Kim, and H. U. Han. 2006. Microbial population dynamics of kimchi, a fermented cabbage product. FEMS Microbiol. Lett. 257: 262-267 https://doi.org/10.1111/j.1574-6968.2006.00186.x
  3. Church, F. C., S. P. Meyers, and V. R. Srinivasan. 1980. Isolation and characterization of genes of alpha-galactosidase from Pichia guilliermondii, pp. 339-348. In L. A. Underkofler and M. L. Wulf (eds.), Developments in Industrial Microbiology, Vol. 21. Lubrecht & Cramer
  4. Han, H. U., C. R. Lim, and H. K. Park. 1990. Determination of microbial community as an indicator of kimchi fermentation. Kor. J. Food Sci. Technol. 22: 26-32
  5. Holt, J. G., N. R. Krieg, P. H. A. Sneath, J. T. Staley, and S. T. Williams. 1994. Bergey's Manual of Determinative Bacteriology, p. 541. 9th Ed. Williams & Wilkins, Baltimore, Maryland
  6. Jeong, S. J., J. Y. Park, H. J. Lee, and J. H. Kim. 2007. Characterization of pFMBL1, a small cryptic plasmid isolated from Leuconostoc mesenteroides SY2. Plasmid 57: 314-323 https://doi.org/10.1016/j.plasmid.2006.09.003
  7. Jeong, S. J., J. Y. Park, J. H. Kim, G. M. Kim, J. Chun, J. H. Lee, D. K. Chung, and J. H. Kim. 2006. Transformation of Leuconostoc mesenteroides SY1, a strain isolated from kimchi. J. Microbiol. Biotechnol. 16: 149-152
  8. Kim, J. H., J. Y. Park, S. J. Jeong, J. Chun, J. H. Lee, D. K. Chung, and J. H. Kim. 2005. Characterization of the ${\alpha}$-galactosidase gene from Leuconostoc mesenteroides SY1. J. Microbiol. Biotechnol. 15: 800-808
  9. Kim, M. H., S. T. Shim, Y. S. Kim, and K. H. Kyung. 2002. Diversity of Leuconostoc on garlic surface, an extreme environment. J. Microbiol. Biotechnol. 12: 497-502
  10. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 https://doi.org/10.1038/227680a0
  11. Lee, H. J., S. Y. Park, and J. Kim. 2000. Multiplex PCRbased detection and identification of Leuconostoc species. FEMS Microbiol. Lett. 193: 243-247 https://doi.org/10.1111/j.1574-6968.2000.tb09431.x
  12. Park, J., M. Lee, J. Jung, and J. Kim. 2005. pIH01, a small cryptic plasmid from Leuconostoc citreum IH3. Plasmid 54: 184-189 https://doi.org/10.1016/j.plasmid.2005.03.003
  13. Park, J. Y., J. S. Park, J. H. Kim, S. J. Jeong, J. Chun, J. H. Lee, and J. H. Kim. 2005. Characterization of the catabolite control protein (CcpA) gene from Leuconostoc mesenteroides SY1. J. Microbiol. Biotechnol. 15: 749-755
  14. Park, J. Y., S. J. Jeong, J. Chun, J. H. Lee, D. K. Chung, and J. H. Kim. 2006. Characterization of ptsHI operon from Leuconostoc mesenteroides SY1, a strain isolated from kimchi. J. Microbiol. Biotechnol. 16: 988-992