Expression of ${\alpha}$-Galactosidase Gene from Leuconostoc mesenteroides SY1 in Leuconostoc citreum

  • Park, Jae-Yong (Institute of Argriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Seon-Ju (Institute of Argriculture and Life Science, Gyeongsang National University) ;
  • Lee, Ae-Ran (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Park, Ji-Yeong (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Jeong, Woo-Ju (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Kim, Jeong-Hwan (Institute of Argriculture and Life Science, Gyeongsang National University)
  • Published : 2007.12.31

Abstract

A 2.5 kb aga gene encoding ${\alpha}$-galactosidase (${\alpha}$-Gal) from Leuconostoc mesenteroides SY1 was cloned into pSJE, an E. coli-Leuconostoc shuttle vector. The recombinant plasmid, pSJEaga, was introduced into Leuconostoc citreum KCTC3526 (ATCC49370) by electroporation. Transcription level of aga was the highest in cells grown on raffinose (1%, w/v) followed by cells grown on galactose, melibiose, fructose, glucose, and sucrose. Western blot using antibodies against ${\alpha}$-Gal showed similar results to slot-blot results and enzyme activity measurements. All the results indicated that the aga was successfully expressed in L. citreum and its transcription was under the carbon catabolite repression (CCR).

Keywords

References

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