Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Structural Determination of Squalene Synthase Inhibitor from Prunus mume Fruit

  • Choi, Sung-Won (Department of Food and Culinary Art, Osan College) ;
  • Hur, Nam-Yoon (Department of Food and Culinary Art, Osan College) ;
  • Ahn, Soon-Cheol (Department of Microbiology and Immunology, and Medical Research Institute, Pusan National University College of Medicine) ;
  • Kim, Dong-Seob (College of Natural Resources and Life Sciences, Pusan National University) ;
  • Lee, Jae-Kwon (Department of Foods and Biotechnology, Kyonggi University) ;
  • Kim, Dae-Ok (Institute of Life Science and Resources, Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Park, Seung-Kook (Institute of Life Science and Resources, Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Byun-Yong (Institute of Life Science and Resources, Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Baik, Moo-Yeol (Institute of Life Science and Resources, Department of Food Science and Biotechnology, Kyung Hee University)
  • Published : 2007.12.31
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Abstract

Squalene synthase plays an important role in the cholesterol biosynthetic pathway. Inhibiting this enzyme in hypercholesterolemia can lower not only plasma cholesterol but also plasma triglyceride levels. A squalene synthase inhibitor was screened from Prunus mume fruit, and then purified via sequential processes of ethanol extraction, HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, and crystallization. The squalene synthase inhibitor was identified as chlorogenic acid with a molecular mass of 354 Da and a molecular formula of $C_{16}H_{18}O_9$ based on UV spectrophotometry, $^1H$ and $^{13}C$ NMRs, and mass spectrometry. Chlorogenic acid inhibited the squalene synthase of pig liver with an $IC_{50}$ level of 100 nM. Since chlorogenic acid was an effective inhibitor against the squalene synthase of an animal source, it may be a potential therapeutic agent for hypercholesterolemia.

Keywords

References

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