Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
권호 표지

BONE HEALING CAPACITY OF THE NEW FLUORIDATED HYDROXYAPATITE IN THE RABBIT CRANIUM DEFECT

가토 두개골 결손부에 이식된 새로운 합성 골이식재의 골치유능력

  • Rho, Kyu-Seop (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University) ;
  • Han, Se-Jin (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University) ;
  • Kim, Chul-Hwan (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University) ;
  • Kim, Kyung-Wook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University)
  • 노규섭 (단국대학교 치과대학 구강악안면외과학교실) ;
  • 한세진 (단국대학교 치과대학 구강악안면외과학교실) ;
  • 김철환 (단국대학교 치과대학 구강악안면외과학교실) ;
  • 김경욱 (단국대학교 치과대학 구강악안면외과학교실)
  • Published : 2007.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

The bone graft materials are grossly divided into autogenous bone, allogenic bone, xenogenic bone, and alloplastic material. Among the various allogenic graft materials, hydroxyapatite($Ca_{10}(PO_4)_6(OH)_2$, HA), the main inorganic phase of human hard tissue, is widely used as a repair material for bones. When HA applied to bony defect, however, it may be encapsulated with fibrous tissue and floated in the implanted area by the lack of consolidation. Fluoridated hydroxyapatite($Ca_{10}(PO_4)_6(OH)_2$, FHA), where F- partially replaces the OH- in the hydroxyapatite, is considered as an alternative material for bone repair due to its solubility and biocompatibility. This study was designed to find out the bone healing capacity of FHA newly produced as a nanoscale fiber in the laboratory. We implanted HA and FHA in the rabbit cranium defect and histologically analysed the specimen. The results were as follows. 1. In the 4 weeks, fibrous connective tissue and little bone formation around materials of the experimental group I implanted HA were observed. In the experimental group II implanted FHA, newly formed bone around materials were observed. 2. In the 8 weeks, the amount of newly formed and matured bone of the experimental group II was more than the experimental group I and control group. From the results obtained, we suggest that FHA, newly synthesized, is relatively favorable bone substitute with bioconpatibility and has better bone healing capacity than pure HA.

Keywords

References

  1. Boyne PJ: Induction of bone repair by various bone grafting materials, hard tissue growth, repair and remineralization. Ciba Found Symp 1973;11:121
  2. Wagner JR: A 3 1/2-year clinical evaluation of resorbable hydroxylapatite osteogen (HA Resorb) used for sinus lift augmentations in conjunction with the insertion of endosseous implants. J Oral Implantol 1991;17:152
  3. Wagner JR: A clinical and histological case study using resorbable hydroxyapatite for the repair of osseous defects prior to endosseous implant surgery. J Oral Implantol 1989;15:186
  4. Costantino PD, Friedman CD: Synthetic bone graft substitutes. Otolaryngol Clin North Am 1994;27:1037
  5. Prokic B, Carranza FA, Kenny EB, et al.: Comparative clinical study of porous hydroxyapatite and decalcified freeze-dried bone in human periodontal defects. J Periodontol 1990;61:399
  6. Papay FA, Morales L, Ahmed OF, et al.: Comparison of ossification of demineralized bone, hydroxyapatite, Gelfoam, and bone wax in cranial defect repair. J Craniofac Surg 1996;7:347 https://doi.org/10.1097/00001665-199609000-00006
  7. Okazaki M. Miake Y, Tohda H, et al.: Functionally graded fluoridated apatites. Biomaterials 1999;20:1421-6 https://doi.org/10.1016/S0142-9612(99)00049-6
  8. Driessens FCM: Relation between apatite solubility and anticariogenic: effect of fluoride. Nature 1973;243:420-1 https://doi.org/10.1038/243420a0
  9. Moreno EC, Kresak M, Zahradnik RT: Fluoridated hydroxyapatite solubility and caries formation. Nature 1974;247:64-65 https://doi.org/10.1038/247064a0
  10. LeGeros RZ, Silverstone LM, Daculsi G, et al.: In vitro caries-like lesion formation in F-containing tooth enamel. J Dent Res 1985;62:138-144
  11. Marie PJ, De Vernejoul MC, LomIi A: Stimulation of bone formation in osteoporosis patients treated with fluoride associated with increased DNA synthesis by osteoblastic cells in vitro. J Bone Miner Res 1992;7:103-113 https://doi.org/10.1002/jbmr.5650070115
  12. Laurencin CT, Ambrosio AMA, Borden MD, et al.: Tissue engineeIing: orthopedic applications. Annu Rev Biomed Eng 01:19-46, 1999 https://doi.org/10.1146/annurev.bioeng.1.1.19
  13. Kim H: J Biomed Mater Res B Appl Biomater 2006;77(2):323-8
  14. Glowacki J, Mulliken JB: Induced osteogenesis for repair and construction in the craniofacial region. Plast Reconstr Surg 1980;65:553 https://doi.org/10.1097/00006534-198005000-00001
  15. Glowacki J, Kaban LB, Mulliken JB: Treatment of jaws defects with demineralized bone implants. J Oral Maxillfac Surg 1982;40:623 https://doi.org/10.1016/0278-2391(82)90109-4
  16. Glowacki J, Mulliken JB: Demineralized bone implants. Clin Plast Surg 1985;12:233
  17. Burstein AH, Einhorn TA, Kopman CR, et al.: The healing of segmental bone defects induced by demineralized bone matrix. J Bone Joint Surg 1984;66:274
  18. Masters DH: Implants. Bone and bonesubstitutes. Calif Dent Assoc J 1988;16:56
  19. Jarcho M: Calcium phosphate ceramics as hard tissue prosthetics. Clin Orthop 1981;157:259
  20. Dee KC, Puleo DA, Bizios R: An introduction to tissue-biomaterial interactions. New York, NY: John Wiley & Sons; 2002
  21. Kim HW, Lee EJ, Kim HE, Salih V, et al.: Effect of fluoridation of hydroxyapatite in hydroxyapatite-polycaprolactone composites on osteoblast activity. Biomaterials 2005;26:4395-4404 https://doi.org/10.1016/j.biomaterials.2004.11.008
  22. Kim HW, Kim HE, Salih V, et al.: Hydroxyapatite and fluor-hydroxyapatite layered film on titanium processed by a sol-gel route for hard-tissue implants. J Biomed Mater Res Part B: Appl Biomater 2004;71B:66-76 https://doi.org/10.1002/jbm.b.30064
  23. Kim HW, Li LH, Koh YH, et al.: Sol-gel preparation and properties of fluoride-substituted hydroxyapatite powders. J Am Ceram Soc 2004;87:1939-1944
  24. Li D, Xia Y: Electrospinning of nanofibers: reinventing the wheel? Adv Mater 2004;16:1151-1170 https://doi.org/10.1002/adma.200400719
  25. Dzenis Y: Spinning continuous fibers for nanotechnology.: Science 2004;304:1917-1919 https://doi.org/10.1126/science.1099074