Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Improved Detection of Mycobacterium leprae by One-tube Nested Polymerase Chain Reaction

  • Wang, Hye-Young (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Whang, Joo-Hwan (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Kim, Jong-Pill (Affiliated Hospital, Korean Leprosy Control Association) ;
  • Cho, Jang-Eun (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Bang, Hye-Eun (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Lee, Hye-Young (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Cho, Sang-Nae (Department of Microbiology, Yonsei University College of Medicine)
  • Published : 2007.12.31
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Abstract

One-tube nested polymerase chain reaction (PCR) was evaluated for its efficacy in detecting Mycobacterium leprae in biopsy samples from leprosy patients. Primers were derived from the M leprae-specific element (RLEP) sequences which yield a 230 bp fragment. The specificity and the sensitivity of the one-tube nested PCR were compared with those of single PCR for detecting M leprae. The results showed that the one-tube nested PCR was about 100 times more sensitive than that of the single indicating the one-tube nested primer sets developed in this study can be an effective screening tool for the detection of M leprae in clinical diagnostic laboratories.

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