한국자기공명학회논문지 (Journal of the Korean Magnetic Resonance Society)
- 제11권2호
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- Pages.85-94
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- 2007
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- 1226-6531(pISSN)
Structural Study of the Cytosolic C-terminus of Vanilloid Receptor 1
- Seo, Min-Duk (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
- Won, Hyung-Sik (Department of Biotechnology, Division of Life Sciences, College of Biomedical & Health Science, Konkuk University) ;
- Oh, Uh-Taek (Sensory Research Center, CRI, Seoul National University) ;
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Lee, Bong-Jin
(Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
- 발행 : 2007.12.20
초록
Vanilloid receptor I [transient receptor potential vanilloid subfamily member 1 (TRPV1), also known as VR1] is a non-selective cationic channel activated by noxious heat, vanilloids, and acid, thereby causing pain. VR1 possesses six transmembrane domain and N-and C-terminus cytosolic domains, and appears to be a homotetramer. We studied the structural properties of Cterminus of VR1 (VR1C) using CD and NMR spectroscopy. DPC micelles, with a zwitterionic surface, and SDS micelles, with a negatively charged surface, were used as a membrane mimetic model system. Both SDS and DPC micelles could increase the stability of helical structures and/or reduce the aggregation form of the VR1C. However, the structural changing mode of the VR1C induced by the SDS and DPC micelles was different. The changes according to the various pHs were also different in two micelles conditions. Because the net charges of the SDS and DPC micelles are negative and neutral, respectively, we anticipate that this difference might affect the structure of the VR1C by electrostatic interaction between the surface of the VR1C and phospholipids of the detergent micelles. Based on these similarity and dissimilarity of changing aspects of the VR1C, it is supposed that the VR1C probably has the real pI value near the pH 7. Generally, mild extracellular acidic pH (
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