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Anti-CD3 Antibody Induces IL-10-producing $CD4^+CD25^+$ Regulatory T Cells, Which Suppress T Cell Response in Rheumatoid Arthritis Patients

  • Yoon, Bo-Young (Department of Internal Medicine, Inje Univeresity Ilsan Paik Hospital) ;
  • Cho, Mi-La (Division of Rheumatology, Department of Internal Medicine, The Center for Rheumatic Diseases, and The Rheumatism Research Center (RhRC), Catholic Research Institutes of Medical Sciences, Catholic University of Korea) ;
  • Hong, Yeon-Sik (Division of Rheumatology, Department of Internal Medicine, The Center for Rheumatic Diseases, and The Rheumatism Research Center (RhRC), Catholic Research Institutes of Medical Sciences, Catholic University of Korea) ;
  • Jhun, Joo-Yeon (Division of Rheumatology, Department of Internal Medicine, The Center for Rheumatic Diseases, and The Rheumatism Research Center (RhRC), Catholic Research Institutes of Medical Sciences, Catholic University of Korea) ;
  • Park, Mi-Kyung (Division of Rheumatology, Department of Internal Medicine, The Center for Rheumatic Diseases, and The Rheumatism Research Center (RhRC), Catholic Research Institutes of Medical Sciences, Catholic University of Korea) ;
  • Park, Kyung-Su (Division of Rheumatology, Department of Internal Medicine, The Center for Rheumatic Diseases, and The Rheumatism Research Center (RhRC), Catholic Research Institutes of Medical Sciences, Catholic University of Korea) ;
  • Park, Sung-Hwan (Division of Rheumatology, Department of Internal Medicine, The Center for Rheumatic Diseases, and The Rheumatism Research Center (RhRC), Catholic Research Institutes of Medical Sciences, Catholic University of Korea) ;
  • Kim, Ho-Youn (Division of Rheumatology, Department of Internal Medicine, The Center for Rheumatic Diseases, and The Rheumatism Research Center (RhRC), Catholic Research Institutes of Medical Sciences, Catholic University of Korea)
  • Published : 2007.09.30
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Abstract

Background: Regulatory T cells (Tregs) have been investigated intensively for some decades. These cells regulate the immune system, prevent overactivated immune responses and can be used therapeutically. For rheumatoid arthritis (RA), understanding the functions and status of Tregs is an important step for understanding immune regulation in this autoimmune disease. Methods: We investigated the percentages, phenotypes and suppressive functions of $CD4^+CD25^+$ Tregs in peripheral blood (PB) of patients with RA. Results: The percentages were higher in the patients (n=12) than in healthy controls (n=10), and the cells expressed the $CD45RB^{low}$, CTLA-4 and CCR7 phenotypes. We also investigated the expression of Foxp3 and secretion of interleukin (IL)-10 induced $CD4^+CD25^+$ Tcells by anti-CD3 antibody treatment. A suppressive function of the patients' cells was shown through coculture with $CD4^+CD25^-$ T cells in vitro. Conclusion: We suggest that, despite their increased numbers and suppressive function, they manage the ongoing inflammation ineffectively. It might be possible to apply IL-10 to induce the proliferation of IL-10-producing Tregs as therapy for RA.

Keywords

References

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