Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Hypolipidemic effect of Salicornia herbacea in animal model of type 2 diabetes mellitus

  • Hwang, Ji-Yeon (Biohealth Product Research Center, School of Food and Life Science, Institute for Food Sciences, Institute of Biomedical Engineering, Inje University) ;
  • Lee, Soo-Kyung (Biohealth Product Research Center, School of Food and Life Science, Institute for Food Sciences, Institute of Biomedical Engineering, Inje University) ;
  • Jo, Ja-Rim (Biohealth Product Research Center, School of Food and Life Science, Institute for Food Sciences, Institute of Biomedical Engineering, Inje University) ;
  • Kim, Mi-Eun (Biohealth Product Research Center, School of Food and Life Science, Institute for Food Sciences, Institute of Biomedical Engineering, Inje University) ;
  • So, Hyun-Ah (Dept. of Genetic Engineering, Dong-A University) ;
  • Cho, Chang-Woo (Dept. of Genetic Engineering, Dong-A University) ;
  • Seo, Young-Wan (Dicision of Ocean Science, Korea Maritime University) ;
  • Kim, Jung-In (Biohealth Product Research Center, School of Food and Life Science, Institute for Food Sciences, Institute of Biomedical Engineering, Inje University)
  • Published : 2007.12.31
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Abstract

To control blood glucose level as close to normal is a major goal of treatment of diabetes mellitus. Hyperglycemia and hyperlipidemia are the major risk factors for cardiovascular complications, the major cause of immature death among the patients with type 2 diabetes. The purpose of this study is to determine the hypoglycemic and hypolipidemic effects of Salicornia herbacea in animal model of type 2 diabetes and to investigate the possible mechanisms for the beneficial effects of S. herbacea. S. herbacea was extracted with 70% ethanol and desalted with 100% ethanol. Three week-old db/db mice (C57BL/KsJ, n=16) were fed AIN-93G semipurified diet or diet containing 1% desalted ethanol extract of S. herbacea for 6 weeks after 1 week of adaptation. Fasting plasma glucose, triglyceride, and total cholesterol were measured by enzymatic methods and blood glycated hemoglobin ($HbA_{1C}$) by the chromatographic method. Body weight and food intake of S. herbacea group were not significantly different from those of the control group. Fasting plasma glucose and blood glycated hemoglobin levels tended to be lowered by S. herbacea treatment. Consumption of S. herbacea extract significantly decreased plasma triglyceride and cholesterol levels (p<0.05). The inhibition of S. herbacea extract against yeast ${\alpha}$-glucosidase was 31.9% of that of acarbose at the concentration of 0.5 mg/mL in vitro. The inhibitory activity of ethanol extract of S. herbacea against porcine pancreatic lipase was 59.0% of that of orlistat at the concentration of 0.25 mg/mL in vitro. Thus, these results suggest that S. herbacea could be effective in controlling hyperlipidemia by inhibition of pancreatic lipase in animal model of type 2 diabetes.

Keywords

References

  1. American Diabetes Association (1999). Management of dyslipidemia in adults with diabetes (Position Statement). Diabetes Care 22: 56-59 https://doi.org/10.2337/diacare.22.1.56
  2. Bailey CJ & Day C (1989). Traditional plant medicines as treatments for diabetes. Diabetes Care 12:553-564 https://doi.org/10.2337/diacare.12.8.553
  3. Ballinger A & Peikin SR (2002). Orlistat: its current status as an anti-obesity drug. Eur J Pharmacol 440:109-117 https://doi.org/10.1016/S0014-2999(02)01422-X
  4. Bang MA, Kim HA & Cho YJ (2002). Hypoglycemic and antioxidant effect of dietary hamcho powder in streptozotocin-induced diabetic rats. Journal of the Korean Society of Food Science and Nutrition 31:840-846 https://doi.org/10.3746/jkfn.2002.31.5.840
  5. Bate KL & Jerums G (2003). 3: Preventing complications of diabetes. Med J Aust 179:498-503
  6. Bitou N, Ninomiya M, Tsujita T & Okuda H (1999). Screening of lipase inhibitors from marine algae. Lipids 34:441-445 https://doi.org/10.1007/s11745-999-0383-7
  7. Centers for Disease Control and Prevention (1999). Diabetes Surveillance Report, Atlanta, GA: US Department of Health and Human Services
  8. Jang HS, Kim KR, Choi SW, Woo MH & Choi JH (2007). Antioxidant and antithrombus activities of enzyme-treated Salicornia herbacea extracts. Ann Nutr Metab 51:119-125 https://doi.org/10.1159/000100826
  9. Kaneto H, Nakatani Y, Kawamori D Miyatsuka T, Matsuoka TA, Matsuhisa M & Yamasaki Y (2005). Role of oxidative stress, endoplasmic reticulum stress, and c-Jun N-terminal kinase in pancreatic ${\beta}$-cell dysfunction and insulin resistance. Int J Biochem Cell Biol 37:1595-1608 https://doi.org/10.1016/j.biocel.2005.04.003
  10. Kim HY (2007c). Effect of onion (Allium cepa) skin extract on pancreatic lipase and body weight-related parameters. Food Science Biotechnology 16:434-438
  11. Kim KR, Jang MJ, Choi SW, Woo MH & Choi JH (2006). Consumption of water extract of enzyme-treated S. herbacea powder showed hypocholestrolemic effect in rat fed high cholesterol diet. Journal of the Korean Society of Food Science and Nutrition 35:55-60 https://doi.org/10.3746/jkfn.2006.35.1.055
  12. Kim MH (2007a). Effects of Salicornia herbacea L. supplementation on blood glucose and lipid metabolites in sterptozotocin-induced diabetic rats. Korean Journal of Nutrition 40:5-13
  13. Kim MH (2007b). Effects of Salicornia herbacea L. supplementation on lipid peroxidation and mineral levels in streptozotocin-induced diabetic rats. Korean Journal of Nutrition 40:403-412
  14. Kwon CS, Sohn HY, Kim SH, Kim JH, Son KH, Lee JS, Lim JK & Kim JS (2003). Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents. Biosci Biotechnol Biochem 67:1451-1456 https://doi.org/10.1271/bbb.67.1451
  15. Lee HJ, Kim YA, Ahn JW, Lee BJ, Moon SG & Seo YW (2004). Screening of peroxynitrite and DPPH radical scavenging activities from salt marsh plants. Korean Journal of Biotechnology and Bioengineering 19:57-61
  16. Lucas CP, Boldrin MN & Reaven GM (2003). Effect of orlistat added to diet (30% of calories from fat) on plasma lipids, glucose, and insulin in obese patients with hypercholesterolemia. Am J Cardiol 91:961-964 https://doi.org/10.1016/S0002-9149(03)00112-7
  17. Oh JH, Kim EO, Lee SK, Woo MH & Choi SW (2007). Antioxidant activities of the ethanol extract of hamcho (Salicornia herbacea L.) cake prepared by enzymatic treatment. Food Science Biotechnology 16: 90-98
  18. Park SH, Ko SK, Choi JG & Chung SH (2006). Salicornia herbacea prevents high fat diet-induced hyperglycemia and hyperlipidemia in ICR mice. Arch Pharm Res 29:256-264 https://doi.org/10.1007/BF02969402
  19. UK Prospective Diabetes Study (UKPDS) Group (1998). Tight blood pressure control and risk of macrovascular and microvascular complications in type 2 diabetes. UKPDS 38. BMJ 317:703-713 https://doi.org/10.1136/bmj.317.7160.703
  20. Watanabe J, Kawabata J, Kurihara H & Niki R (1997). Isolation and identification of ${\alpha}$-glucosidase inhibitors from Tochu-cha (Encommia ulmoides). Biosci Biotechnol Biochem 61:177-178 https://doi.org/10.1271/bbb.61.177