Journal of Soil and Groundwater Environment (한국지하수토양환경학회지:지하수토양환경)

Korean Society of Soil and Groundwater Environment (한국지하수토양환경학회)
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PAHs Degrading Bacterium Separation and Identification for Biological Treatment

PAHs의 생물학적 처리를 위한 분해 미생물 분리 동정

  • Kim, Man (Department of environment Engineering, Chonnam National University) ;
  • Choi, Kyoung-Kyoon (Department of environment Engineering, Chonnam National University) ;
  • Go, Myong-Jin (Department of environment Engineering, Chonnam National University) ;
  • Park, Jeong-Hun (Department of environment Engineering, Chonnam National University)
  • 김만 (전남대학교 공과대학 환경공학과) ;
  • 최경균 (전남대학교 공과대학 환경공학과) ;
  • 고명진 (전남대학교 공과대학 환경공학과) ;
  • 박정훈 (전남대학교 공과대학 환경공학과)
  • Published : 2007.12.31
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Abstract

Pseudomonas sp. KM1 was separated from soil contaminated by petroleum and identified. The isolated strain is Gram-positive, rod-shaped and immotile. In batch culture, the optimum cultivation temperature and pH was $35^{\circ}C$ and 7, respectively. Biodegradation of PAHs experiment with soil slurry system was performed using Pseudomonas sp. KM1. Pseudomonas sp. KM1 could degrade 7 PAHs including naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, pyrene, and fluoranthene. These mixed PAHs was easily degraded within one day except fluoranthene, which was degraded much slowly, taking several days by this isolated bacteria. Pseudomonas sp. KM1 is good candidate for bioremediation of PAHs contaminated soils. Biodegradation rates of naphthalene, phenanthrene and pyrene in soils were different at each soil, and the rates were decreased as sorption capacity increased.

토양에 존재하는 다핵방향족탄화수소(Polycyclic Aromatic Hydrocarbons, PAHs)의 처리를 위하여 자연계로부터 분리된 균주는 Pseudomonas sp.로 동정되었으며, 이 균주를 KM1으로 명명하였다. 균주의 최적 성장조건은 회분식 배양에서 $35^{\circ}C$, pH 7로 나타났다. 분리균주 Pseudomonas sp. KM1에 의한 7-PAHs(naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, fluoranthene and pyrene)의 분해실험결과 배양 1일 만에 fluoranthene을 제외한 naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene and pyrene 이 분해됨을 확인할 수 있었다. 그리고 토양유무에 따른 PAHs 분해실험 결과, 흡착분배계수와 유기물함량(%)이 큰 신동방이 경방이나 봉동보다 분리균주에 의한 생분해율(%)이 낮았다. 토양에 오염된 유기화합물의 분배특성과 토양 내 유기물함량(%)이 오염된 토양의 생물학적 처리효과에 영향을 미치는 중요한 인자인 것으로 나타났다.

Keywords

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