International Journal of Oral Biology

  • 제32권3호
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  • Pages.113-118
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  • 2007
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
권호 표지

Anti-proliferative and Apoptosis Inducing Effect of Momordin I on Oral Carcinoma (KB) Cells

  • Seo, Kyeong-Seong (Dept. of Oral Maxillofacial Surgery, School of Dentistry, Kyung Hee University) ;
  • Kim, Jeong-Hee (Dept. of Oral Biochemistry, School of Dentistry and Institute of Oral Biology, KyungHee University) ;
  • Kim, Yeo-Gab (Dept. of Oral Maxillofacial Surgery, School of Dentistry, Kyung Hee University)
  • 발행 : 2007.09.30
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초록

Treatment of oral cancers with chemotherapeutic agents become evaluated as an effective method to reduce cancer cell proliferation. Anti-proliferative and anti-oral cancer activities of momordin I on oral cancer cells were evaluated in this study. Momordin I was originally purified from a natural product, Ampelopsis radix and showed the antiproliferative activity against oral carcinoma, KB cells. Obtained $IC_{50}$ value was approximately $10.4{\mu}g/ml$. Time-and dose-dependent chromosomal DNA fragmentations were observed in momordin I-treated KB cells. Flow cytometry analysis showed time-dependent apoptotic cell appearance after treatment of momordin I. Approximately 18.6% apoptotic cells were observed at 72 hours after $20{\mu}g/ml$ of momordin I treatment. These observation were consistent with the results obtained in DNA fragmentation analysis. These data suggest that momordin I has anti-proliferative effect and induces cell death in KB cells through apoptosis.

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