Development of Biologically Active Compounds from Edible Plant Sources - XXV. Immunostimulating Effect of Edible Plant Extracts

식용 식물자원으로부터 활성물질의 탐색 - XXV. 식용 식물 추출물의 면역증강 효과

In this study, the 163 edible plants that are permitted as foods by the Korea Food and Drug Administration, were extracted in methanol. Following extration, their macrophage immunostimulating effects (MIE) were examined using a macrophage from BALB/C mice at four different concentrations of plant extract, such as $10\;{\mu}g/mL$, $100\;{\mu}g/mL$, $500\;{\mu}g/mL$, and $1,000\;{\mu}g/mL$, in vivo. Forty-two samples significantly showed MIEs. Among them, 20 samples had increased MIEs at higher than 10%, as compared to the negative control group. Nineteen samples had increased MIEs at two of the plant extract concentrations, and 3 samples [Allium schoenoprasmum (chive), Aralia elata (fatsia), Capsosiphon fulvescens (seaweed fulvescens)] had increased MIEs at three of the concentration conditions. In particular, Dioscorea batatas (yam) showed MIEs at all concentrations, as well as slightly higher MIEs as compared to the positive control group. Therefore, Dioscorea batatas was evaluated to be an excellent MIE.

식품 소재의 국내산 약용식물인 163가지 천연물에서 메탄올로 추출한 시료를 이용하여 in vivo에서 10, 100, 500, $1000\;{\mu}g/mL$의 4가지 농도에서 대식세포 면역 활성을 측정하였다. 그 결과, 42개의 시료에서 면역증진반응을 보였으며, 그 중 20개의 시료는 음성대조군에 대하여 20% 이상 면역 활성을 증진시키는 것으로 측정되었다. 이중 2가지 농도에서 면역증진반응을 보이는 시료는 총 19개, 3가지 농도에서 면역증진효과를 나타내는 시료는 총 3개[골파(Allium schoenoprasmum), 두릅(Aralia elata), 매생이(Capsosiphon fulvescens)]였으며, 특히 마(Dioscorea batatas)는 각 농도에서 활성을 나타내었을 뿐 아니라 양성대조군과 비슷하거나 높은 활성을 나타내어 면역증진활성이 매우 우수한 것으로 나타났다.