생명과학회지 (Journal of Life Science)

  • 제17권1호
  • /
  • Pages.162-165
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  • 2007
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

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Vibrio tapetis의 검출을 위한 PCR specific primer의 제작

PCR Specific Primer for the Detection of Vibrio tapetis

  • 김영진 (부산대학교 의과대학 미생물학 및 면역학 교실) ;
  • 이선이 (부산대학교 의과대학 미생물학 및 면역학 교실) ;
  • 조효진 (부산대학교 의과대학 미생물학 및 면역학 교실) ;
  • 유선녕 (부산대학교 의과대학 미생물학 및 면역학 교실) ;
  • 김철민 (부산대학교 의과대학 생화학 교실) ;
  • 최용락 (동아대학교 생명자원과학대학 응용생명 공학부) ;
  • 박병근 ((재)바이오21센터) ;
  • 안순철 (부산대학교 의과대학 미생물학 및 면역학 교실)
  • Kim, Yeong-Jin (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Lee, Sun-Yi (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Cho, Hyo-Jin (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • You, Sun-Nyung (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Kim, Cheol-Min (Department of Biochemistry, College of Medicine, Pusan National University) ;
  • Choi, Yong-Lark (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-a University) ;
  • Park, Byoung-Keun (Bio21 Center) ;
  • Ahn, Soon-Cheol (Department of Microbiology and Immunology, College of Medicine, Pusan National University)
  • 발행 : 2007.01.29
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초록

Brown Ring Disease (BRD) is a bacterial disease caused by Vibrio tapetis which affects cultured clam Ruditapes philippinarum and causes heavy economic losses on Atlantic coasts of france, Spain and England. In this study, to evaluate the effective detection of the pathogen, specific primer set based on 16S ribosomal RNA (rRNA) sequences designed for rapid detection of V. tapetis. Polymerase chain reaction (PCR) with this primer set produced the specific band for each V. tapetis. The length of PCR product using designed primer set of Vbts-F and Vbts-R was about 400 bp. Therefore, these primers will be provided with a basic tool for rapid detection of V. tapetis in the various cases such as examination of imported aquatic products, diagnosis of aquatic organisms, and etc.

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참고문헌

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