Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Characterization of DNA Damaging Agent Sensitivity of rqh1 mutant from Schizosaccharomyce pombe

분열형 효모인 Schizosaccharomyces pombe 로부터 rqh1 돌연변이의 DNA damaging agent sensitivity를 보상하는 유전자의 특성 연구

  • Lee, In-Hye (Department of Biological Science, College of Medical Life Science, Silla University) ;
  • Choi, In-Soon (Department of Biological Science, College of Medical Life Science, Silla University)
  • Published : 2007.01.29
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Abstract

The Rqh1 gene is essential for vegetative growth in fission Yeast. The rqh1 mutant showed that sensitivity of DNA damaging agent, a wild range of phenotype including abnormal gene expression and cell elongation. This result showed that the rqhl-overexpression cell was sensitivity to DNA damaging agent like rqhl mutant. When Rqh1 have an over-expression by $nmt1^+$ promoter of pREP vector, rqh1 mutant DNA damaging agent sensitivity could be compensated. We isolated two strong mutant containing complementation gene, rqh156 and rqh172, respectively. This result observed that the DNA damaging agent sensitivity of rqhl mutant was complemented by the expression of rqh156 and rqh172. They induced mRNA expression in a dose-dependent manner HU, MMS and UV. The HU sensitivity of the rqhl was complemented by the expression of rqh156 and rqh172. The mRNA expression of rqh156 decreased on HU dose dependent but the mRNA expression of rqh172 did not decrease on HU dose dependent. The MMS and W sensitivity of the rqhl was complemented by the expression of rqh156 and rqh172. These results indicate that the isolated rqhl gene may play an important role in DNA metabolism.

분열형 효모에서 Rqh1은 Top3과 함께 vegetative growth에 필수적이다. $rqh^-$ 돌연변이는 DNA damaging agent에 민감성을 보이는데 이때, 부적절한 유전자 발현, 세포 신장, 염색체의 불안전성, 비정상적인 다중격막, 발아의 결핍을 포함한 넓은 범위의 표현형을 보인다. rqh1-overexpression cell 역시 rqh1 deletion mutant에서 보이는 DNA damaging agent 민감성을 관찰할 수 있다. 논문은 nmtl promoter를 가지는 PREP vector에 Rqhl이 과발현 할 때 나타나는 DNA damaging agent 민감성를 보상하는 유전자를 찾아 $rqh1^+$의 기능을 알아보는 것이다. 여기서 보상능이 보이는 rqh156, rqh172 두 개의 돌연변이를 골라냈다. rqhl deletion mutant의 DNA damaging agent 민감성은 rqh156, rqh172의 발현에 의해 보상 되어지는 것을 확인하였다.

Keywords

References

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