Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Regulation of cell size and cell number by LANCEOLATA1 gene in Arabidopsis

애기장대의 세포 크기와 세포 수를 조절하는 LANCEOLATA1 유전자

  • Cho, Kiu-Hyung (Division of Moleular Biotechnology, Dong-A University) ;
  • Jun, Sang-Eun (Division of Moleular Biotechnology, Dong-A University) ;
  • Jeong, Soon-Jae (Division of Moleular Biotechnology, Dong-A University) ;
  • Yi, Young-Byung (Division of Moleular Biotechnology, Dong-A University) ;
  • Kim, Gyung-Tae (Division of Moleular Biotechnology, Dong-A University)
  • 조규형 (동아대학교 분자생명공학부) ;
  • 전상은 (동아대학교 분자생명공학부) ;
  • 정순재 (동아대학교 분자생명공학부) ;
  • 이영병 (동아대학교 분자생명공학부) ;
  • 김경태 (동아대학교 분자생명공학부)
  • Published : 2007.01.29
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Abstract

The Precesses for leaf development in dicotyledonous plants are surprisingly complex, while the mechanism of controlling and coordinating them is poorly understood. To characterize the fundamental features of the leaf development of Arabidopsis, we first attempted to isolate mutants that alter leaf morphology. Here, leaf morphological mutant of Arabidopsis, lanceolatal (lan1) which has small and narrow leaves have isolated and characterized. To clarify the function of LAN1 in organ development, we characterized lan1-7 mutant using an anatomical and genetic approach. The lan1-7 mutant had reduced size of foliage leaves and reduced dimensions of stems. A reduction both in cell size and in cell number was evident at the cellular level in the lan1 mutant, revealing that LAN1 gene appears to affect cell division at an earlier stage and cell elongation throughout the development of leaf primordia. from the analysis of heterogeneous plant with lan1 mutation and 35S-AG transgenic plant, AG gene is revealed to regulate leaf morphology under the control of 35S promoter. Thus, MADS-box gene was revealed to have some relationship to that of LAN1 gene at certain stage in leaf development processes.

쌍자엽 식물에 있어서 잎의 발달에 관한 과정은 매우 복잡하게 얽혀있지만, 이를 조절하는 메커니즘에 대해서는 아직 잘 알려져 있지 않다. 모델식물인 애기장대의 잎의 발달의 기초과정을 해명하기 위하여 잎의 형태이상 돌연변이체를 이용한 접근방법을 시도하였다. 본 연구에서 잎이 작고 가는 lanceolata1 돌연변이체를 선별하였고, 이원인유전자의 기능을 밝히기 위하여 유전학적 및 해부학적인 연구를 실시하였다. lan1-7 돌연변이체는 잎 뿐만아니라 줄기도 가늘어지는 표현형을 나타내었고, 이는 세포수준에서 세포크기와 세포수의 감소에 의한 조절되고 있음이 밝혀졌다. 이 결과는 LAN1 유전자가 잎의 발달과정에서 세포분열과 세포신장을 제어하고 있다는 사실을 시사하고 있다. lan1-7 돌연변이체와 35S-AG 형질전환체와의 F1 세대의 표현형 분석을 통하여, AG 유전자가 잎이 가늘고 위로 말리는 모양을 제어하고 있다는 사실이 판명되었다. 이 결과로 MADS-box유전자가 LAN1 유전자에 의해서 제어되고 잎의 발달과정에 관여하고 있다는 사실이 판명되었다.

Keywords

References

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