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채소종자 발아와 유묘생장에 미치는 유기 또는 무기게르마늄의 효과 및 흡수특성

Uptake Properties of Germanium to Vegetable Plants and Its Effect on Seed Germination and on Early Stage Growth

  • 한명자 (순천대학교 생명환경과학부) ;
  • 김성은 (순천대학교 생명환경과학부) ;
  • 서동철 (미국 루이지애나 주립대학 Wetland Biogeochemistry 연구소) ;
  • 정용화 (순천대학교 생명환경과학부) ;
  • 이도진 (순천대학교 농업교육과) ;
  • 박문수 (순천대학교 산림자원조경학부) ;
  • 임요섭 (순천대학교 생명환경과학부) ;
  • 손보균 (순천대학교 생명환경과학부) ;
  • 허종수 (경상대학교 농생명학부) ;
  • 조주식 (순천대학교 생명환경과학부)
  • Han, Myung-Ja (Department of Bio-Environmental Science, Sunchon National University) ;
  • Kim, Sung-Un (Department of Bio-Environmental Science, Sunchon National University) ;
  • Seo, Dong-Cheol (Wetland Biogeochemistry Institute, Louisiana State University) ;
  • Cheong, Yong-Hwa (Department of Bio-Environmental Science, Sunchon National University) ;
  • Lee, Do-Jin (Department of Agricultural Education, Sunchon National University) ;
  • Park, Moon-Su (Department of Forest Resource, Sunchon National University) ;
  • Rim, Yo-Sup (Department of Bio-Environmental Science, Sunchon National University) ;
  • Sohn, Bo-Kyoon (Department of Bio-Environmental Science, Sunchon National University) ;
  • Heo, Jong-Soo (Division of Applied Life Science, Gyeongsang National University) ;
  • Cho, Ju-Sik (Department of Bio-Environmental Science, Sunchon National University)
  • 발행 : 2007.09.30

초록

식물의 종자발아와 유묘생장에 미치는 무기게르마늄과 유기게르마늄의 효과와 흡수특성을 조사하기 위하여, 대표적인 채소류인 갓, 배추 및 청경채를 이용하여 다양한 게르마늄농도(0, 10, 25, 50, 100 mg $L^{-1}$)를 처리하였다. 채소 종자발아는 모든 식물에서 무기게르마늄을 100 mg $L^{-1}$ 처리한 경우에만 약간의 저해를 받았고 나머지 조건에서는 거의 영향을 받지 않았다. 식물의 유묘생장에 미치는 게르마늄의 효과는 갓과 배추의 경우에는 모든 농도의 무기게르마늄($10{\sim}100$ mg $L^{-1})$에서 뿌리의 생장에 심각한 저해를 보였고 지상부 생장은 높은 농도(50, 100 mg $L^{-1}$)에서는 저해를 받지만 낮은 농도(10, 25 mg $L^{-1}$)에서는 오히려 약간의 생장 촉진효과를 보이거나 거의 차이가 없는 것으로 보였다. 유기게르마늄의 처리시에는 뿌리와 지상부의 생육이 낮은 농도(10, 25, 50 mg $L^{-1}$)의 대부분 조건에서 생장을 촉진하는 결과를 보였다. 식물체내의 게르마늄의 흡수 특성은 무기 및 유기게르마늄의 모든 조건에서 대조구에 비해 처리농도가 높아짐에 따라 점진적으로 뚜렷한 증가를 보였다 특히 무기게르마늄을 처리한 경우 보다 유기 게르마늄을 처리한 경우에 같은 농도에서 전반적으로 약 $2\sim4.5$배까지 많이 흡수되었다. 25 mg $L^{-1}$농도의 게르마늄을 처리한 경우를 보면, 유기게르마늄의 처리시 게르마늄의 흡수는 갓에서는 약 4배(무기게르마늄: 0.37 mg $g^{-1}dw$, 유기게르마늄 : 1.47 mg $g^{-1}dw$), 배추에서는 약 2.2배(무기게르마늄: 0.40 mg $g^{-1}dw$, 유기게르마늄: 0.86 mg $g^{-1}dw$) 그리고 청경채의 결우 약 2.1배(무기게르마늄 : 0.33 mg $g^{-1}dw$, 유기게르마늄: 0.70 mg $g^{-1}dw$) 정도로 무기게르마늄의 처리보다 높았다.

To investigate effects of inorganic $(GeO_2)$ and organic (Ge-132) germanium (Ge) on seed germination and on early stage growth of plane and the uptake characteristics, various concentrations (0, 10, 25, 50, 100 mg $L^{-1}$) of Ge to popular vegetables such as leaf mustard, chinese cabbage and pak-choi, respectively, were treated. On seed germination, no significant effect was observed in both inorganic and organic Ge treatments except 100 mg $L^{-1}$ treatment of inorganic Ge. Exogenous inorganic Ge ($10{\sim}100$ mg $L^{-1}$ treatments significantly inhibited the early root elongation growth of all plants. However, slight enhancement of early shoot elongation was detected in low concentrations (10 and 25 mg $L^{-1}$) of Ge in the leaf mustard and chinese cabbage plants. Organic Ge treatments significantly stimulated the 개ot and shoot growth at the 10, 25 and 50 mg $L^{-1}$ treatments. Ge was accumulated linearly in the vegetables as both inorganic and organic Ge concentrations were increased. Interestingly, total contents of Ge in plants with Ge-132 treatments were $2\sim4.5$ times more than those with inorganic Ge treatments in all concentrations. At 25 mg $L^{-1}$ treatment of Ge, contents of Ge in vegetables are following: in leaf mustard, inorganic Ge: 0.37 mg $g^{-1}dw$ and organic Ge: 1.47 mg $g^{-1}dw;$ in the chinese cabbage, inorganic Ge: 0.4 mg $g^{-1}dw$ and organic Ge: 0.86 mg $g^{-1}dw;$ in the pak-choi, inorganic Ge: 0.33 mg $g^{-1}dw$ and organic Ge: 0.70 mg $g^{-1}dw$, respectively. These results showed organic Ge is much better on early stage seedling growth and on germanium accumulation of vegetables than inorganic Ge.

키워드

참고문헌

  1. Greenwald, T. (1998) Is it good medicine? Time 30, 37-44
  2. Obara, K, Saito, T., Sato, H., Yamakage, K, Watanabe, T., Kakizawa, M., Tsukamoto, T., Kobayashi, K, Hongo, M and Yoshinaga, K (1991) Germanium poisoning; clinical symptoms and renal damage caused by long-term intake of germanium. Japanese J. Medicine 30(1), 67-72 https://doi.org/10.2169/internalmedicine1962.30.67
  3. Iijima, M, Mugishima, M, Takeuchi, M, Uchiyama, 5., Kobayashi, I. and Maruyama, S. (1990) A case of inorganic germanium poisoning with peripheral and cranial nephropathy. Myopathy and autonomic dysfunction 42(9), 851-856
  4. Jang, J. J., Cho, K J., Lee, Y. S. and Bae. J. H. (1991) Modifying responses of allyl sulfide, indole3-carbinol and germanium in a rat multi-organ carcinogenesis model. Carcinogenesis 12(4), 691-695 https://doi.org/10.1093/carcin/12.4.691
  5. Mochizuki, H. and Kada, T. (1982) Antimutagenic effect of Ge-132 on $\gamma$-ray-induced mutation in Escherchia coli B/rWP2 trp-. Int. J. Radiat. Biol. 42(6), 653-659 https://doi.org/10.1080/09553008214551621
  6. Suzuki, F., Brutkiewicz, R. R. and Pollard, R. B. (1986) Cooperation of lymphokine (s) and marcophages in expression of antitumor activity of carboxyethylgermanium (Ge-132). Antitumor Res. 62(2), 177-182
  7. Aso, H., Suzuki, F., Yamaguchi, T., Hayashi, Y., Ebina, T. and Ishida, N. (1985) Induction of interferone and activation of NK cells and macrophages in mice by oral administration of Ge-12, and organic germanium compound. Microbiol. Immunol. 29(1), 65-74 https://doi.org/10.1111/j.1348-0421.1985.tb00803.x
  8. Dimartino, M. J. (1986) Antiarthritic and immunoregulatory activity of spirogermanium. J. Pharmacol. Exp. Ther. 236(1), 103-110
  9. Sasaki, K., Ishikawa, M., Monma, K. and Takayanagi, G. (1984) Effect of carboxyethylgennanium sesquioxide (Ge-132) on the acute inflammation and $CCl_4$ induced hepatic damage in mice. Pharmacometrics 27(6), 1119-1131
  10. Kumano, N., Nakai, Y., Ishikawa, T., Koinumaru, S., Suzuki, S. and Konno, K. (1978) Effect of carboxyethylgermanium sesquioxide in the methylcholathrene induced tumorigenesis. Sci. Rep. Res. Inst. Tohoku Univ. 25, 89-95
  11. Harish, G. (1985) Glutathione and glutathionedependent enzymes of the rat liver after different doses of sanumgerman. In '1st Int. Conf. on germanium' Hanover, Oct. 1984 Lekin & Samochowiec, des, Semmelweis-Verlag
  12. Suzuki, Y. and Taguchi, K. (1983) Pharmacological studies of carboxyethylgermanium sesquioxide (Ge132). Pharmacometrics 26(5), 803-810
  13. Lee, H. M. and Chung, Y. (1991) Effect of organic germanium on metallothionnein inductin in liver and kindey of cadmium and mercury intoxicated rats. Yakhak Hoeji 35(2), 99-110
  14. Ho, C. C, Cherm, Y. F. and Lin, M. T. (1990) Effects of organogermanium compound 2carboxyethylgermanium sesquioxide on cardiovascular function motor activity in rats. Pharmacology 41, 286-291 https://doi.org/10.1159/000138736
  15. Lee, S.T., Lee, Y. H., Lee, H. J., Cho, J. S. and Heo, J. S. (2005) Germanium contents of soil and crops in Gyeongnam province. Korean J. Environ. Agric. 24(1), 34-39 https://doi.org/10.5338/KJEA.2005.24.1.034
  16. Kehlbeck, H. (1983) New germanium containing yeast for medicinal and veterinary use. Deutsch Patent DE. 3345211
  17. Nobohiro, W., Osamu, I., Dakuro, K. and Koichi, Y. (1980) New approaches to using spent brewer's yeast. ASBC J. 38, 5
  18. Wei, X. S. (1992) Effect of yeast on bioenrichment of germanium. Food Sci. 149, 49-54
  19. Lee, S. T., Lee, Y. H., Choi, Y. J., Lee, S. D., Lee, C. H. and Heo, J. S. (2005) Growth characteristics and germanium absorption of rice plant with different germanium concentration in soil. Korean J. Environ. Agric. 24(1), 40-44 https://doi.org/10.5338/KJEA.2005.24.1.040
  20. Lee, S. T., Lee, Y. H., Bhan, K. N., Seo, D. C. and Heo, J. S. (2005) Growth characteristics and germanium absorption in Lettuce with different concentrations of germanium in soil. Korean J. Environ. Agric. 24(4), 404-408 https://doi.org/10.5338/KJEA.2005.24.4.404
  21. Lee, G. P., Park, H. S., Won, J. H. and Park, K. W. (2005) Effect of $GeO_2$ concentration on hydroponically-grown lettuce (Lactuca sativa). J. Kor. Soc. Hart. Sci. 46(2), 113-118
  22. Han, S. S., Rim, Y. S. and Jeong, J. H. (1996) Growth characteristics and germanium absorption of soybean sprout cultured with aqueous solution of organogermanium. Agric. Chern. Biotech. 39(1), 39-43
  23. Chang, E, J. and Oh, H. I. (2005) Effects of addition of inorganic germanium, $GeO_2$ on the growth, germanium and saponin contents of ginseng adventitious root in submerged culture. J. Ginseng Res. 29(3), 145-151 https://doi.org/10.5142/JGR.2005.29.3.145

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  3. Accumulation and Toxicity of Germanium in Cucumber under Different Types of Germaniums vol.44, pp.20, 2013, https://doi.org/10.1080/00103624.2013.829083
  4. Effects of Ge-132 and GeO2 on seed germination and seedling growth of Oenothera biennis L. under NaCl stress vol.38, pp.1, 2017, https://doi.org/10.1080/09593330.2016.1186226
  5. Effects of GeO2 on chlorophyll fluorescence and antioxidant enzymes in apple leaves under strong light vol.56, pp.4, 2018, https://doi.org/10.1007/s11099-018-0807-7