Effect of Additives on the Conidial Viability of Aspergillus sp. PS-104

Aspergillus sp. PS-104의 분생포자 생활력에 미치는 첨가제 효과

A fungus, Aspergillus sp. PS-104, with the high phosphate-solubilizing activities was isolated from Korean upland soil and formulated into a solid powder type with various additives. For the long-time preservation of conidia, some additives (Tween 80, SDS, Triton X-100, glucose, glycerol, corn oil, bio-ceramic, PEG 200, $Cu^{++}$, $Mo^{+++}$, $Fe^{++}$, $Ca^{++}$ and $Zn^{++}$) were supplemented in the rice-cooked hard medium with various concentrations (0, 0.001, 0.01, 0.1, 1.0 and 5.0%). In case of surfactants. the highest relative viability of the Aspergillus sp. PS-104 conidia was recorded nearly to 80% by the addition of 0.01 to 0.1% Tween 80, while 50% in control. The number of conidia were found to be about 100 times higher when treated at 0.01 to 0.1% Tween 80 as compared to control. Relative viability of the conidia was decreased in order of Tween 80 $\geq$ SDS > Triton X-100 during the storage at $25^{\circ}C$. As regards the organic additives, the relative viability of Aspergillus sp. PS-104 conidia was also recorded nearly to 80% by the addition of 1.0% bio-ceramic, and 5.0% glucose and sucrose during the storage at $25^{\circ}C$. In case of metal ions, the relative viability of Aspergillus sp. PS-104 conidia was decreased in order of $Cu^{2+}>Ca^{2+}>Mo^{3+}>Zn^{2+}>Fe^{2+}$ during the storage at $25^{\circ}C$.

포자의 장기보존 풍 생활력 유지를 위하여 계면활성제(Tween 80, SDS, Triton X-100) 및 2가 금속이온 $Ca^{2+}$, $Fe^{2+}$, $Cu^{2+}$, $Zn^{2+}$ 및 3가 금속이온 $Mo^{3+}$을 각각 0, 0.001, 0.01, 0.1, 1.0%(v/v) 농도로 첨가하여 생활력에 미치는 영향을 조사하였다. 또한 각종 유기물(PEG 200, glycerol, 식용유, 종합 vitamin, 설탕, 당밀)도 각각 0, 0.01, 0.1, 1.0, 5.0%(v/v) 농도로 첨가하여 2주, 4주, 2개월, 4개월, 6개월간 보관하면서 생활력을 검정하였다. 계면활성제 중 Tween 80을 $0.01%\sim1.0%$ 농도로 첨가하여 6개월 저장시 상대생존율이 80%에 이르렀으며, 이에 비해 대조구는 약 50% 수준이었다. 이때 분생포자수는 대조구에 비해서 약 100배 높은 수준이었다. 계면활성제 처리 후 저장기간에 따른 균의 생활력은 Tween $80{\geq}SDS>Triton$ X-100 순으로 감소하였다. 유기물 중에서 1.0% 맥반석, 5.0% glucose 및 설탕을 첨가하였을 때가 장기보존시 우수함을 확인하였으며 6개월 경과시 대조구는 초기에 비해 50%의 상대생존율을 보였으나 1.0% 맥반석 첨가시 상대생존율은 약 80%의 높은 수준을 유지하였다. 각종 금속이온을 0.1% 농도로 처리 후 저장기간에 따른 균의 생활력은 $Cu^{2+}>Ca^{2+}>Mo^{3+}>Zn^{2+}>Fe^{2+}$ 순으로 감소하였다.