International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Inhibitory Effect of Buthus martensi Karsch Extracts on ${\alpha}$-Glucosidase Enzyme

  • Kim, Eun-Ok (Department of Biological Engineering, Seokyeong University) ;
  • Kim, Shin-Duk (Department of Biological Engineering, Seokyeong University)
  • Published : 2007.12.31
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Abstract

While searching for ${\alpha}$-glucosidase inhibitors, the active compound was found in a methanol extract of Burthus martensi Kirsch. The separation of the active compound was performed using various chromatography methods and the physico-chemical properties of the purified compound were characterized. The compound showed very potent inhibitory activity against ${\alpha}-glucosidase$ with an $IC_{50}$ value of $5.3\;{\mu}g/ml$. Lineweaver-Burk plot indicated that its inhibition of ${\alpha}-glucosidase$ was competitive.

Keywords

References

  1. Asano, N. (2003) Glycosidase inhibitors: Update and perspectives on practical use. Glycobiology. 13, 93R-104R https://doi.org/10.1093/glycob/cwg090
  2. Becerril, B., A. Vazquez, C. Garcia, M. Corona, F. Bolivar and L.D. Possani (1993) Cloning and characterization of cDNAs that code for $Na^+$ -channel blocking toxins of scorpion Centruroides noxius Hoffmann. Gene 128, 165-171 https://doi.org/10.1016/0378-1119(93)90559-L
  3. Chapel, C., C. Garcia, P. Roingeard, N. Zitzmann, J. Dubuisson, R. A. Dwek, C. Trepo, F. Zoulim and D. Durante (2006) Antiviral effect of a-glucosidase inhibitors on viral morphogenesis and binding properties of the hepatitis C virus-like particles. J. Gen. Vir. 87, 861-871 https://doi.org/10.1099/vir.0.81503-0
  4. Elbein, A. (1987) Inhibitors of the biosynthesis and processing of Nlinked oligosaccharide chains. Ann. Rev. Biochem. 56, 497-534 https://doi.org/10.1146/annurev.bi.56.070187.002433
  5. Fischer, P., M. Collin, G. Karlsson, W. James, T. Butters, S. Davis, S. Gordon, R. Dwek and F. Platt (1995) The a-glucosidase inhibitor, N-butyldeoxynojirimycin inhibits human immunodeficiency virus entry at the level of post-CD4 binding. J. Vir. 69, 5791-5797
  6. Jacob, G. (1995) Glycosylation inhibitors in biology and medicine. Curr. Opin. Struct. Biol. 5, 605-611 https://doi.org/10.1016/0959-440X(95)80051-4
  7. Ji, Y., P. Mansuelle, S. Terakawa, C. Kopeyan, N. Yanaihara, K. Hsu and H. Rochat (1996) Two neurotoxins (BMK I and BMK II) from the venom of the scorpion Buthus Martensi Karsch: Purification, amino acid sequences and assessment of specific activity. Toxicon 34, 987-1001 https://doi.org/10.1016/0041-0101(96)00065-7
  8. Kim, K.S., H. S. Cho, S.D. Lee, K. H. Kim, J. Y. Cho, K. H. Chung, Y. C. Lee, S. K. Moon and C. H. Kim (2005) Inhibitory effect of Buthus martensi Karsch extracts on interleukin- 1b-induced expression of nitric oxide (NO) synthase and production of NO in human chondrocytes and LPS-induced NO and prostaglandin E2 production in mouse peritoneal macrophages. Toxicology in vitro 19, 757-769 https://doi.org/10.1016/j.tiv.2005.04.008
  9. Kornfeld R. and S. Kornfeld (1985) Assembly of asparaginelinked oligosaccharides. Annu. Rev. Biochem. 54, 631-664 https://doi.org/10.1146/annurev.bi.54.070185.003215
  10. Martin, J. L., K. Veluraja, L. N. Ross, G. Johnson, W. J. Fleet, N. G. Ramsden, I. Bruce, M. G. Orchard, N. G. Oikonomakos and A.C. Papageorgiou (1991) Glucose analogue inhibitors of glycogen phosphorylase: design of potential drugs for diabetes. Biochemistry 30, 10101-10116 https://doi.org/10.1021/bi00106a006
  11. Mehta, A., N. Zitzmann, P.M. Rudd, T.M. Block and R.A. Dwek (1998) Alpha-glucosidase inhibitors as potential broad based anti-viral agents. FEBS Letters 430, 17-20 https://doi.org/10.1016/S0014-5793(98)00525-0
  12. Murai, A., K. Iwamura, M. Takada, K. Ogawa, T. Usui and J. Okumura (2002) Control of postprandial hyperglycaemia by galactosyl maltobionolactone and its novel anti-amylase effect in mice. Life Sci. 71, 1405-1415 https://doi.org/10.1016/S0024-3205(02)01844-1
  13. Papandre, M.J., RY. Barbouche, R. E. Guieu, M. Paulekieny and E. Fenouillet (2002) The ${\alpha}-Glucosidase$ Inhibitor 1- Deoxynojirimycin Blocks Human Immunodeficiency Virus Envelope Glycoprotein-Mediated Membrane Fusion at the CXCR4 Binding Step. Mol Pharmacol. 61,186-193 https://doi.org/10.1124/mol.61.1.1
  14. Sunkara, P., T. Bowlin, P. Liu and A. Sjoerdsma (1987) Antiretroviral activity of castanospermine and deoxynojirimycin, specific inhibitors of glycoprotein processing. Biochem. Biophys. Res. Commun. 148, 206-210 https://doi.org/10.1016/0006-291X(87)91096-5
  15. Taylor R. and A.B. Johnson (1996) Does suppression of postprandial blood glucose excursions by the alpha-glucosidase inhibitor miglitol improve insulin sensitivity in diet-treated type II diabetic patients? Diabetes Care, 19, 559-563, 1996 https://doi.org/10.2337/diacare.19.6.559
  16. Tifft, C.J. and R. Proia (2000) Stemming the tide: glycosphingolipid synthesis inhibitors as therapy for storage diseases. Glycobiology 10, 1249-1258 https://doi.org/10.1093/glycob/10.12.1249
  17. Van den Broek, L.A., M.W. Kat-Van Den Nieuwenhof, T.D. Butters, C.A. Van Boeckel (1996) Synthesis of alpha-glucosidase I inhibitors showing antiviral (HIV-1) and immunosuppressive activity. J. Pharm Pharmacol. 48, 172-178 https://doi.org/10.1111/j.2042-7158.1996.tb07117.x
  18. Watson, A., G. Fleet, N. Asano, R. Molyneux and R. Nash (2001) Polyhydroxylated alkaloids-natural occurrence and therapeutic applications. Phytochemistry 56, 265-295 https://doi.org/10.1016/S0031-9422(00)00451-9