Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Induced Expression of Doublesex-and mab-3-related Transcription Factor-1 (DMRT-1) mRNA by Testosterone in the Olive Flounder, Paralichthys olivaceus ovary

Testosterone 처리에 의한 넙치, Paralichthys olivaceus 난소에서 doublesex-and mab-3-related transcription factor-1 (DMRT-1) mRNA의 발현 유도

  • Jo, Pil-Gue (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • An, Kwang-Wook (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Kim, Na-Na (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Choi, Yong-Ki (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Cho, Sung-Hwoan (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Min, Byung-Hwa (Aquaculture Research Team, NFRDI) ;
  • Lim, Han-Kyu (Aquaculture Research Team, NFRDI) ;
  • Choi, Cheol-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
  • 조필규 (한국해양대학교 해양환경.생명과학부) ;
  • 안광욱 (한국해양대학교 해양환경.생명과학부) ;
  • 김나나 (한국해양대학교 해양환경.생명과학부) ;
  • 최용기 (한국해양대학교 해양환경.생명과학부) ;
  • 조성환 (한국해양대학교 해양환경.생명과학부) ;
  • 민병화 (국립수산과학원 수산생명과학본부 양식관리팀) ;
  • 임한규 (국립수산과학원 수산생명과학본부 양식관리팀) ;
  • 최철영 (한국해양대학교 해양환경.생명과학부)
  • Published : 2007.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

We isolated a 317 bp of partial cDNA for doublesex-and mab-3-related transcription factor-1 (DMRT-1) from the testis of olive flounder, Paralichthys olivaceus using RT-PCR. Based on the multiple sequence alignment, olive flounder DMRT-1 shared relatively high sequence homology (82 to 94%) with orthologues from other teleost species such as Atlantic halibut, Hippoglossus hippoglossus, black porgy, Acanthopagrus schlegeli and rainbow trout, Oncorhynchus mykiss. DMRT-1 mRNA was predominantly expressed in the testis of olive flounder. In our investigation for the effect of testosterone treatment in vivo on induced expression of ovarian DMRT-1 transcript, mRNA levels of DMRT-1 in ovary were significantly up-regulated by testosterone treatments (0.3 or $3.0{\mu}g$ testosterone/g body weight for 12 to 36 hours) as judged by RT-PCR analysis. In overall, transcriptional stimulation of DMRT-1 during treatments was more affected by doses of testosterone than treatment durations. This result strongly suggests that the regulation of DMRT-1 be tissue- and gender-specific in olive flounder, and also provides useful baseline knowledge on the testosterone-mediated regulation in the reproductive physiology of this species.

본 연구에서는 넙치(Paralichthys olivaceus) 정소로부터 DMRT-1 partial cDNA를 분리하였다. 넙치 DMRT-1은 317개의 염기로 구성되어 있으며, Atlantic halibut, 감성돔 및 무지개 송어와 각각 94%, 85%, 82%의 높은 상동성을 보였다. RT-PCR을 이용하여 DMRT-1 mRNA 발현은 난소보다 정소에서 높게 나타난 것을 관찰할 수 있었다. 또한, 암컷 넙치에 웅성호르몬인 testosterone 처리시 처리 농도에 따라 난소에서 DMRT-1 mRNA 발현이 증가함을 볼 수 있었다. 따라서, DMRT-1은 수컷에서 특이적으로 발현되는 유전자임을 알 수 있으며, 본 연구의 결과는 넙치의 성 전환 및 유도에 대한 기초자료를 제공할 것이다.

Keywords

References

  1. Bang, I.C., K.K. Kim and Y. Kim, 1996. Sex reversal of olive flounder (Paralichthys olivaceus) by immersion in a solution of steroid hormones. J. Aquaculture, 9, 279-285
  2. Baroiller, J.F., D. Chourrout, A. Fostier and B. Jalabert, 1995. Temperature and sex chromosomes govern sex ratio of mouthbrooding cichlid fish Oreochromis niloticus. J. Exp. Zool., 273, 216-223 https://doi.org/10.1002/jez.1402730306
  3. Brennan, J.J., J. Karl, K. Nordqvist, C. Schmahl, K.U. Tilmann and B. Capel, 1998. SRY and the testis: molecular pathways of organogenesis. J. Exp. Zool., 281, 494-500 https://doi.org/10.1002/(SICI)1097-010X(19980801)281:5<494::AID-JEZ14>3.0.CO;2-9
  4. Devlin, R.H. and Y. Nagahama, 2002. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture, 208, 191-364 https://doi.org/10.1016/S0044-8486(02)00057-1
  5. Fernandino, J.L., L.G. Guilgur, P.H. Strobl-Mazzulla and G.M. Somoza, 2003. Molecular cloning of SOX9, DMRT-1 and SF-1 cDNA partial sequences in the pejerrey fish Odontesthes bonariensis (Atheriniformes). Fish Physiol. Biochem., 28, 145-146 https://doi.org/10.1023/B:FISH.0000030503.86575.f3
  6. Fernandino, J.I., L.G. Guilgur and G.M. Somoza, 2006. Dmrt1 expression analysis during spermatogenesis in pejerrey, Odontesthes bonariensis. Fish Physiol. Biochem., 1493, 180-187
  7. Guan, G., T. Kobayashi, Y. Nagahama, 2000. Sexually dimorphic expression of two types of DM (doublesex/Mab-3)-domain genes in a teleost fish, the tilapia (Oreochromis niloticus). Biochem. Biophys. Res. Commun., 272, 662-666 https://doi.org/10.1006/bbrc.2000.2840
  8. Hunter, G.A. and E.M. Donaldson, 1983. Production of monosex female groups of chinook salmon (Oncorhynchus tshawytscha) by the fertilization of normal ova with sperm from sex-reversed females. Aquaculture, 33, 355-364 https://doi.org/10.1016/0044-8486(83)90414-3
  9. Kettlewell, J.R., C.S. Raymond and D. Zarkower, 2000. Temperature- dependent expression of turtle Dmrt1 prior to sexual differentiation. Genesis, 26, 174-178 https://doi.org/10.1002/(SICI)1526-968X(200003)26:3<174::AID-GENE2>3.0.CO;2-J
  10. Kim B.S., Y.B. Moon, C.H. Jeong, D.S. Kim and Y.D. Lee, 1994. Evaluation of fertility of artificial induced gynogenetic diploid male in Paralichthys olvaceus. J. Aquaculture, 7, 151-158
  11. Kitano, T., K. Takamune, Y. Nagahama and S.I. Abe, 2001. Role of P450 aromatase in gonadal sex differentiation in Japanese flounder (Paralichthys olivaceus). Environ. Sci., 1, 1-11 https://doi.org/10.1076/evms.1.1.1.23762
  12. Kobayashi, T., M. Matsuda, H. Kajiura-Kobayashi, A. Suzuki, N. Saito, M. Nakamoto, N. Shibata and Y. Nagahama, 2004. Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes. Dev. Dyn., 231, 518-526 https://doi.org/10.1002/dvdy.20158
  13. Marchand, O., M. Govoroun, H. D'Cotta, O. McMeel, J. Lareyre, A. Bernot, V. Laudet and Y. Guiguen, 2000. DMRT1 expression during gonadal differentiation and spermatogenesis in the rainbow trout, Oncorhynchus mykiss. Biochim. Biophys. Acta, 1493, 180-187 https://doi.org/10.1016/S0167-4781(00)00186-X
  14. Melamed, P., H. Rosenfeld, A. Elizur and Z. Yaron, 1998. Endocrine regulation of gonadotropin and growth hormone gene transcription in fish. Comp. Biochem. Physiol., 119, 325-338 https://doi.org/10.1016/S0305-0491(97)00357-X
  15. Nakamua, M., T. Kobayashi, X. Chang and Y. Nagahama, 1998. Gonadal sex differentiation in teleost fish. J. Exp. Zool., 281, 362-372 https://doi.org/10.1002/(SICI)1097-010X(19980801)281:5<362::AID-JEZ3>3.0.CO;2-M
  16. Raymond, C.S., C.E. Shamu, M.M. Shen, K.J. Seifert, B. Hirsch, J. Hodgkin and D. Zarkower, 1998. Evidence for evolutionary conservation of sex-determining genes. Nature, 391, 691-695 https://doi.org/10.1038/35618
  17. Raymond, C.S., J.R. Kettlewell, B. Hirsch, V.J. Bardwell and D. Zarkower, 1999. Expression of Dmrt1 in the Genital Ridge of Mouse and Chicken Embryos Suggests a Role in Vertebrate Sexual Development. Dev. Biol., 215, 208-220 https://doi.org/10.1006/dbio.1999.9461
  18. Shibata, K., M. Takase and M. Nakamura, 2002. The Dmrt1 expression in sex-reversed gonads of amphibians. Gen. Comp. Endocrinol., 127, 232-241 https://doi.org/10.1016/S0016-6480(02)00039-4
  19. Smith, C.A., P.J. McClive, P.S. Western, K.J. Reed and A.H. Sinclair, 1999. Conservation of a sex-determining gene. Nature, 402, 601-602
  20. Veith, A.M., A. Froschauer, C. Koorting, I. Nanda, R. Hanel, M. Schmid, M. Scharmid and J. Volff, 2003. Cloning of the dmrt1 gene of Xiphophorus maculatus: dmY/dmrt1Y is not master sex-determining gene in the platyfish. Gene., 317, 59-66 https://doi.org/10.1016/S0378-1119(03)00664-4
  21. Zhu, L., J. Wilken, N.B. Phillips, U. Narendra, G. Chan, S.M. Stratton, S.B. Kent and M.A. Weiss, 2000. Sexual dimorphism in diverse metazoans is regulated by a novel class of intertwined zinc fingers. Genes. Dev., 14, 1750-1764