Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Production of Induced Gynogenetic Diploid Bagrid Catfish Leiocassis ussuriensis (Siluriformes) - I. Optimization of Treatment Condition for Diploid Gynogenesis

대농갱이 Leiocassis ussuriensis (Siluriformes) 자성발생성 이배체 생산 - I. 자성발생성 이배체 유도 처리 조건의 최적화

  • Park, Sang-Yong (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Lee, Yoon-A (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
  • 박상용 (순천향대학교 해양생명공학과) ;
  • 이윤아 (순천향대학교 해양생명공학과) ;
  • 남윤권 (부경대학교 양식학과) ;
  • 방인철 (순천향대학교 해양생명공학과)
  • Published : 2007.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Treatment conditions for the induced diploid gynogenesis, a maternal-exclusive form of artificial parthenogenetic reproduction, were optimized in bagrid catfish (Leiocassis ussuriensis, Siluriformes). Optimal amounts of ultraviolet (UV) irradiation for the genetic inactivation of spermatozoa in bagrid catfish and Pseudobagrus fulvidraco were proven to be ranged from 3,600 to 4,800 $ergs/mm^2$ based on the examination of viability of embryos and haploid incidence. Haploid embryos were restored to diploidy by preventing the extrusion of the second polar body using cold shock treatment. Thermal treatments (4 or $6^{\circ}C$ for 30, 40 or 50 min) were carried out 3, 5 or 7 min post insemination. Best scores for embryo viability (38.6% of total eggs taken) and incidence of normal diploidy (87.9% of hatched larvae) were observed at the embryo group treated at $4^{\circ}C$ for 40 min, 5 min after insemination. Restoration of gynogenetic diploidy was confirmed based on the absence of haploid syndrome, cell size and/or nucleolar organizing region (NOR) counts.

대농갱이, Leiocassis ussuriensis의 유전육종학적 연구의 일환으로 자성발생성 이배체 유도 기법을 최적화하였다. 대농갱이 반수체 유도를 위해 다양한 농도의 자외선 조사를 통해 대농갱이 및 동자개 정자의 유전학적 불활성화 실시하였으며 최적 자외선 처리 조건은 4,800 $ergs/mm^2$로 나타났고, 이 때 Hertwig effect와 함께 97% 이상의 반수체 유도가 가능하였다. 반수체 대농갱이는 전형적인 반수체 증후군(haploid syndrome)을 보였으며, 반수체 대농갱이를 이배체로 복원시키기 위해 $4^{\circ}C$ 또는 $6^{\circ}C$에서 인공 수정 후 3, 5 및 7분 후에 30, 40 및 50분 간의 처리를 수행한 결과, 가장 높은 생존율(38.6%)과 이배체 복원률(87.9%)은 수정 5분 후 $4^{\circ}C$에서 40분간 처리 조건에서 관찰되었다.

Keywords

References

  1. Arai, K., 2001. Genetic improvement of aquaculture finfish species by chromosome manipulation techniques in Japan. Aquaculture, 197, 205-228 https://doi.org/10.1016/S0044-8486(01)00588-9
  2. Arai, K., T. Masaoka and R. Suzuki, 1992. Optimum conditions of UV ray irradiation for genetic inactivation of loach eggs. Nippon Suisan Gakkaishi, 58, 1197-1201 https://doi.org/10.2331/suisan.58.1197
  3. Chourrout, D. and E. Quillet, 1982. Induced gynogenesis in the rainbow trout : sex and survival of progenies. Production of all-triploid population. Theor. Appl. Genet., 63, 201-205 https://doi.org/10.1007/BF00303993
  4. Galbusera. P., F. A. M. Volckaert and F. Ollevier, 2000. Gynogenesis in the African catfish Clarias gariepinus (Burchell, 1822) III. Induction of endomitosis and the presence of residual genetic variation. Aquaculture, 185, 25-42 https://doi.org/10.1016/S0044-8486(99)00339-7
  5. Im, J. H., H. J. Cho, Y. K. Nam, D. S. Kim and I.-S. Park, 2001. Production of gynogenetic diploid in the far eastern catfish, Silurus asotus. Korean J. Genet., 23, 89-101
  6. Jeong, C. H., Y. B. Moon, I.-S. Park and D. S. Kim, 1996. $F_2$ production of gynogenetic diploid in olive flounder, Paralichthys olivaceus. J. Aquaculture, 9, 287-291
  7. Kim, D. S., J. H. Kim, J.-Y. Jo, Y. B. Moon and K. C. Cho, 1993. Induction of gynogenetic diploid in Paralichthys olivaceus. Korean J. Genet., 15, 179-186
  8. Lee, C.-L., and I.-S. Kim, 1990. A taxonomic revision of the family bagridae (Pisces, Siluriformes) from Korea. Korean J. Ichthyol., 2, 117-137
  9. Lin, F. and K, Dabrowski, 1998. Androgenesis and homozygous gynogenesis in muskellunge (Esox masquinongy): evaluation using flow cytometry. Mol. Repro. Dev., 49, 10-18 https://doi.org/10.1002/(SICI)1098-2795(199801)49:1<10::AID-MRD2>3.0.CO;2-R
  10. Nam, Y. K., C. H. Noh and D. S. Kim, 2006. Intraspecies androgenesis in mud loach (Misgurnus mizolepis): I. Optimization of the egg inactivation and haploid androgenesis using transgene marker. J. Aquaculture, 19, 166-172
  11. Nam, Y. K., H. J. Cho, J. H. Im, I. S. Park, G. C. Choi and D. S. Kim, 2001. Production of all-female diploid and triploid far eastern catfish, Silurus asotus (Linnaeus): survival and growth performance. Aquacult. Res., 32, 991-997 https://doi.org/10.1046/j.1365-2109.2001.00634.x
  12. Nam, Y. K., Y. S. Cho and D. S. Kim, 2000. Isogenic transgenic homozygous fish induced by artificial parthenogenesis. Transgenic Res., 9, 463-469
  13. Pandian, T. J. and R. Koteeswaran, 1998. Ploidy induction and sex control in fish. Hydrobiologia, 384, 167-243 https://doi.org/10.1023/A:1003332526659
  14. Park, S. Y.,, D.-J. Kim, Y.-A. Lee, C. H. Noh, D. S. Kim and I. C. Bang, 2006. Cytogenetic analysis of an intergeneric hybrids between Korean bullhead (Pseudobagrus fulvidraco) and Ussurian bullhead (Leiocassis ussuriensis). Kor. J. Ichthyol. 18, 20-26
  15. Thogaard, G. H. and S. K. Allen Jr., 1987. Chromosome manipulation and markers in fishery management. In Population Genetics and Fisheries Management, N. Ryman and F. M. Utter (eds.). University of Washington Press, Seattle, pp 319-331
  16. Thorgaard, G. H., 1986. Ploidy manipulation and performance. Aquaculture, 57, 57-64 https://doi.org/10.1016/0044-8486(86)90180-8
  17. Varadi. L., I. Benko, J. Varga and L. Horvath, 1999. Induction of diploid gynogenesis using interspecific sperm and production of tetraploids in African catfish, Clarias gariepinus Burchell (1822). Aquaculture, 173, 401-411 https://doi.org/10.1016/S0044-8486(98)00467-0